LOCUS       sequence-419976-        4455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hcoRBFOX1N-MCP-RBFOX1C(190-397).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Liu Z, Jillette N, Robson P, Cheng AW
  TITLE     Simultaneous multifunctional transcriptome engineering by CRISPR RNA
            scaffold.
  JOURNAL   Nucleic Acids Res. 2023 Aug 11;51(14):e77. doi: 10.1093/nar/gkad547.
  PUBMED    37395412
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2023-08-11"; volume: "51(14):e77. doi"; pages: "
            10.1093/nar/gkad547"
COMMENT     Sequence Label: pmax-hcoRBFOX1N-MCP-RBFOX1C
FEATURES             Location/Qualifiers
     source          1..4455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          813..945
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    960..984
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1386..1772
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     protein_bind    complement(2484..2508)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2561..2602
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(2628..2647)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    2657..2738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2682..2701
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2936..3524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3016..3035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3605..4399)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3605..4399)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3716..3735)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4326..4345
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacagggaaa aaacaattga
      961 caagtttgta caaaaaagca ggctccgaat tcaccggtgc cgccaccatg aattgcgagc
     1021 gggaacagct caggggcaat caagaagcgg cagctgcacc tgacacaatg gcacagccgt
     1081 atgcgagtgc tcaatttgcg ccaccccaga atggcatacc cgcagaatac acagcgccgc
     1141 atcctcaccc cgcccctgaa tacactgggc aaactaccgt tcctgaacac acgcttaacc
     1201 tctatcctcc tgctcagact cactcagaac agagtccagc agatacaagt gcgcagacag
     1261 tctctggcac tgcgacccag accgatgacg cagctcctac tgatggccag ccacaaactc
     1321 aaccctctga aaacactgaa aataaaagcc aacccaaggg cggtggaggc tccgggcgcg
     1381 ccatggcttc aaactttact cagttcgtgc tcgtggacaa tggtgggaca ggggatgtga
     1441 cagtggctcc ttctaatttc gctaatgggg tggcagagtg gatcagctcc aactcacgga
     1501 gccaggccta caaggtgaca tgcagcgtca ggcagtctag tgcccagaag agaaagtata
     1561 ccatcaaggt ggaggtcccc aaagtggcta cccagacagt gggcggagtc gaactgcctg
     1621 tcgccgcttg gaggtcctac ctgaacatgg agctcactat cccaattttc gctaccaatt
     1681 ctgactgtga actcatcgtg aaggcaatgc aggggctcct caaagacggt aatcctatcc
     1741 cttccgccat cgccgctaac tcaggtatct acagcgctgg cggccgcggg ggaggcggtt
     1801 ccggtggcgg cggaagcgga ggtggaggat cagggccggc caatgccacg gctagggtca
     1861 tgacaaacaa gaagacggtt aatccgtata ccaacgggtg gaaacttaat cccgttgtcg
     1921 gcgctgtcta ttctcccgag ttctatgctg gtacagtctt gctgtgccaa gccaatcaag
     1981 aggggtcttc aatgtacagt gctccgagca gcttggttta cacaagcgcg atgccaggat
     2041 ttccttaccc tgcggcaacg gccgcggctg cgtatcgggg tgcccacctt cgcgggcggg
     2101 gccgaacagt ttacaacacg ttcagggccg ctgctccacc tcccccgatt cccgcgtacg
     2161 gaggtgttgt ctaccaagac gggttttatg gagccgacat ctacggtggt tacgccgcat
     2221 atcggtatgc acaaccaacc cccgcgaccg cggcagcgta cagcgactcc tacggacgcg
     2281 tgtacgctgc tgatccttac caccacgccc tggcccccgc cccaacgtat ggtgtcggtg
     2341 ctatgaatgc gttcgcgccc ctgaccgatg caaaaaccag gtcacatgcc gacgacgttg
     2401 gattggtatt gagctctttg caagcatcca tttaccgggg gggatacaac cgcttcgcgc
     2461 cttattgatt aattaagaat tcgacccagc tttcttgtac aaagtggttg atatccagca
     2521 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
     2581 tctcctcggt ctcgattcta cgcgtaccgg ttagcaattg ttttttcgat gagtttggac
     2641 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     2701 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     2761 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     2821 aatgtggtac ttaagagggg gagaccaaag ggcgagacgt taaggcctca cgtgacatgt
     2881 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2941 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3001 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3061 ctgttccgac cctgccgctt acgggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3121 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3181 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3241 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3301 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3361 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3421 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3481 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3541 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     3601 cgtctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     3661 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     3721 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     3781 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     3841 tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg
     3901 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     3961 gagtacgtgc tctctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     4021 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     4081 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     4141 cttcagtgac aacgtcgagt acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     4201 gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     4261 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     4321 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     4381 atccatcttg ttcaatcata atattattga agcatttatc agggttcgtc tcgtcccggt
     4441 ctcctcccat gcatg
//
