LOCUS       sequence-420001-        8649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express gRNA against NANOG with puro and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Ishikawa M, Sugino S, Masuda Y, Tarumoto Y, Seto Y, Taniyama N, 
            Wagai F, Yamauchi Y, Kojima Y, Kiryu H, Yusa K, Eiraku M, Mochizuki 
            A
  TITLE     RENGE infers gene regulatory networks using time-series single-cell 
            RNA-seq data with CRISPR perturbations.
  JOURNAL   Commun Biol. 2023 Dec 28;6(1):1290. doi: 10.1038/s42003-023-05594-4.
  PUBMED    38155269
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-12-28"; volume: "6(1):1290. doi"; pages: " 
            10.1038/s42003-023-05594-4"
COMMENT     Sequence Label: pKLV2-U6gRNA5(NANOG_g1)-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..8649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        243..441
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     396..416
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             459..639
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    686..811
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1310..1543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1728..1772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2070..2187
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2262..2510
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2262..2282
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2433..2452
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2638..3137
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2673..2696)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3070..3089
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3177..3773
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3177..3196)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3673..3693
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3792..3845
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3846..4547
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4607..4627)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5025..5036)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5488..5505)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5494..5718
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5744..5764)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5744..5762)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5783..5799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5783..5799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5796..5818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5807..5823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5831..5837,5838..5855,5856..5861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5876..5897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6014..6031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6185..6773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6265..6284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6944..7735,7736..7804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7567..7586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7805..7909)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7935..8390)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8072..8093)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8284..8303
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8517..8539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8531..8548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8532..8548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8558..8577
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8558..8576
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
       61 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      121 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      181 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      241 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      301 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      361 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      421 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
      481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
      841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
      901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
      961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1141 acagcaagcg gccggccgcg ctgatcttca gacctggagg aggagatatg agggacaatt
     1201 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1261 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1321 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1381 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1441 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1501 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1561 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1621 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1681 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1741 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1801 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1861 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1921 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1981 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2041 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2101 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2161 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2221 tggttagtac cgggccctac gcgtccaagg tcgggcagga agagggccta tttcccatga
     2281 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
     2341 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     2401 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     2461 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gcctcagcta
     2521 caaacaggtg tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc
     2581 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttggatccaa ttctaccggg
     2641 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     2701 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     2761 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     2821 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     2881 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     2941 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     3001 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     3061 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     3121 catctccggg cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga
     3181 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     3241 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     3301 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     3361 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     3421 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     3481 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     3541 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     3601 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     3661 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     3721 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccggcggcg
     3781 ggtccggagg agagggcaga ggaagtctcc taacatgcgg tgacgtggag gagaatcctg
     3841 gcccaatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     3901 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     3961 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     4021 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     4081 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaggacg
     4141 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     4201 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     4261 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     4321 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
     4381 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     4441 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     4501 ccagatactg cgacctccct agcaaactgg ggcacaagct taattgagcg gccgctcaac
     4561 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     4621 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     4681 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4741 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4801 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4861 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4921 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4981 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5041 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctacgccttc
     5101 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcaggtacct ttaagaccaa
     5161 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5221 ggctaattca ctcccaaaga agtcaagatc tgctttttgc ctgtactggg tctctctggt
     5281 tagaccagag tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5341 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5401 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5461 ctctagcagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5521 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5581 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5641 gtggggtggg gcaggacagc aagggggagg attgggaagt caatagcagg catgctgggg
     5701 atgcggtggg ctctatgggc ggccgttaat taagcttttg ttccctttag tgagggttaa
     5761 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5821 caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt gcctaatgag
     5881 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5941 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6001 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6061 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6181 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7081 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7921 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     7981 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     8041 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     8101 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     8161 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     8221 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     8281 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     8341 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     8401 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     8461 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     8521 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     8581 attgactagt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     8641 cccattgac
//
