LOCUS       sequence-420029-       27649 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  A plasmid containing a 17,639 bp fragment of the P. tricornutum 
            chloroplast genome. The fragment is flanked by SapI recognition 
            sites, allowing it to be released from the cloning vector pSAP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 27649)
  AUTHORS   Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
  TITLE     Design and assembly of the 117-kb Phaeodactylum tricornutum 
            chloroplast genome.
  JOURNAL   Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.
  PUBMED    38114089
REFERENCE   2  (bases 1 to 27649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670."
COMMENT     Sequence Label: pCHAP5
FEATURES             Location/Qualifiers
     source          1..27649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8431..8442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        11628..11730
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             11731..12390
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11797..11816)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    12610..12631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12646..12651,12652..12669,12670..12676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12684..12700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             12720..12995
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     misc_feature    12767..12823
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(12825..12844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12826..12844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12851..12868)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12851..12867)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12860..12882)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(13075..13094)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    13162..13195
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    13213..13611
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     CDS             complement(14675..15646)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(15646..16821)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             complement(17217..17891)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(17892..18039)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     misc_feature    18093..18596
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     misc_feature    18758..19008
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(19011..19766)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      19857..20076
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      20144..20675
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      20152..20766
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 acttttttcg ttgcgatagt ttcatttcta atctcactaa ttacaagatt agcttgattt
       61 aattgttttt gtgcttcgtt tagacgtttc tgtgcttcat ttaaacgatc ttcggcatct
      121 tgaacacctt ttacgatcgt tgtttttcgt tcttctaata aagatcctaa aaaatcttta
      181 ccagcataga ttaaaatacc aactaacgtc aaaatattta gtaaacctgt ttcaagaata
      241 ttcagattta aaccaatacc ttcatgttcg gcaagtaatg taaaaatttg atcaaaattt
      301 tccatgtttt cgagttttta tataaactgt tcacttataa aaaggaaaat tacgtttaac
      361 aaaaaattta aattgataat ctagcttcaa tatcgacaca taaagattga acaatttcat
      421 ctaaactatt aagtgcaata ttttttttag caagaagatc tttttgaata gtatctaata
      481 aattatcaat atatttttga gaaatattta attcaacttc taagatttct ttatgaattt
      541 tttgtgagtt tgtaatttct aattgtgctt ctttacgaac gttatctaat tcttgctcat
      601 attgttgagt tagtttgtta gcttctgata acaattcgga agctttaccc agattcgtca
      661 agatatactc tttacgttct tcgataattg ttaataatgg gttatataat aaaatgttta
      721 acaaaaccat taataatata aattgaatag caactaaggg taaagttgcg ttaaaatcaa
      781 ataatccacc agggccacta acttctgaat ttgaaattag cattgaaata ttaatcatac
      841 aaatttatgt attctactat agaattaaaa ttgtttaata aagaaaatca ttaactttgt
      901 taacaaaatt ctagtagttt aaaaaatcaa acagttgttc aaaaactaca aagttaaata
      961 atgtaaaagt taaaatcgat catgactttt acattatttt aaaaatttta tactgttaat
     1021 taagtgttaa acgggttagc gaataataaa gctaatgcta ctactaggcc ataaatagtt
     1081 aaggcttcca tgaacgctaa acttaataat aatacaccac gaattttgtt ttctgcttca
     1141 ggttgacgag caataccctc aaccgcttga ccagcagcag taccttgacc aataccaggc
     1201 ccaattgcag ctaaaccaat cgataaacca gcaccaataa cagaagcagc agaaatgata
     1261 ggatccataa taaactttaa attgtaagta tttaagtaat ttttaaaaaa tctaattgca
     1321 gtaattttac ttaactttat tcaagtgctt ctgcaatata tgcagctgct aaagttgaga
     1381 aaattaaagc ctgtactgaa ccagcaaaaa ctcctaatag cataattggt aatgggataa
     1441 ctaaaggaac taatgatgtt aatactgtaa ttgttaattc atcagctaaa acatttccaa
     1501 ataatcggaa acttaatgat aaaggttttg taaaatcttc taaaatatta atcggtagaa
     1561 gaagtggaag cggttcaata tatcttttga aataccctaa acctttttta ctcaaacctg
     1621 catagaaata agcaaaagag gtcaataaag ctagagcaac cgttgtatta atatcattgg
     1681 ttggagcagc aagctcgccc tccggcaatt ctattaattt ccaagggatg actgcaccag
     1741 cccagttact tccaaagata aagaagaata atgtgccaat aaagggaatc cattctttat
     1801 agaagctttc gcctaattga tctctagcaa tatctgtgac aaagtctact gatgcttcca
     1861 taaaattttg aaaatcatgt ggaatacgat cagcatttag agtacctaga agtgaaaata
     1921 ttaataaaac taatattaca aaccacataa ctgctaaaac ttgaccatgt actagaaatc
     1981 ctgcaatatt ccagtaaaaa tgttttccaa cttccgcctc cgctaataat gtaaacgtat
     2041 ttgaataaaa aatttctggg tacataaaat ttaactaatt tagtaaatta cccaatatta
     2101 aaaaatatac aggaacaact aatattataa gaggcatcga ggtattttag gtttattttt
     2161 ataaaaagac tctatttttt ggaagtttaa gtttctagcc attcataacc tttcgcttcc
     2221 aattccttcg ctaactcagc agagccagtt ttaattattt ttccattttg cattacatga
     2281 acataattgg gattaacata atctaataat cgttgataat gtgtaattaa aataatcgac
     2341 ttttcgggtc ccataaaatt attaattcca tttgaaatta ttttcaaagc gtcaatatcg
     2401 agaccagaat ctgtttcatc taaaatcgat aattcagaat ctaataaaat catttgtaaa
     2461 atttcatttc gttttttttc accaccggaa aaaccttcat taacatttcg gccaagaaat
     2521 ctggctgaca tattaactag ttgtagtttc tgagtgataa tcgtcaaaaa ttctattgga
     2581 tcaacttcta ctttattata gaatttttgc ttagaattat atgctaaacg taaaaagtct
     2641 tcgttactaa cccctggaat ttcaactggg tactgaaacg ctaaaaaaat acctaaatgt
     2701 gaacgttctt caggttcaag atctaaaata cttgaacctt tgaataagat atcaccacct
     2761 aaaattgaat aagctgggtg accagccaaa acttttgaga aagtactttt acctgaccca
     2821 ttcggtccca taatagcatg gatttcgccc ttatttatag ttaaatttag attttttaga
     2881 atctgatttt cattaataga aacttgtaaa tttttgatct ctaatagagg agaatttaaa
     2941 gtcattagcc tacgcttcct tctaatttta aagttaatag tttatctgct tctgcagcaa
     3001 attctaatgg taattcagta aaaatttcac ggcaaaatcc acttatcatt aattcaactg
     3061 ctttttctaa acaaatacca cgttgtaaaa aatagaaaat ttgttcttca ccaattttag
     3121 aagtcgaagc ttcatgttca atctttgttg ttgagttttg aacagaaata aatggaaatg
     3181 tgttagcgtt tgataaatca ccaattaata gtgaatcaca ctgtgaataa tttcttgcac
     3241 ctaaagcttt acttgttaca ttaacaagac cccgataact attttttgat ttaccagcag
     3301 agatgccttt tgaaataatt cgacttcgtg tatttcttcc aatatgcatc attttcgttc
     3361 cggtatctgc ttgctgataa ttattagtta aagcgactga ataaaattca ccctgtgaat
     3421 tgtctccaat aagaacacaa ctaggatatt tccaagtgat tgacgatcca gtttcaactt
     3481 gtgtccacga aattttcgag ctggatcctg cacataaacc gcgtttagtt acaaaattgt
     3541 aaactccacc ttttccaaat tcattacctg cataccaatt ttgcactgtt gaatatttaa
     3601 tatttgcatt ttctagtgca attaattcaa caacagctgc atgtagctga ttactatcat
     3661 attgcggggc tgtgcaacct tcaagataat ttacttgact atttttttct gaaataatta
     3721 acgttctttc aaattgtcca gatttttcgt cattaattcg aaaataggtc gaaagatcta
     3781 gtggacaaat tgtatcttca ggaatataac aaaaagagcc atctgtaaaa actgctgagt
     3841 tcaaagctgc aaaataatta tctccaattg gaacaactga tcccaagtat ttttcaatta
     3901 attcaggata ttcagtaact gcttctgaaa ttgaagaaaa aattacacct gatttattta
     3961 attcttcctt aaaagtggtt ccaattgaga cactatcgaa aactacatca acagcgacat
     4021 tagctaaacg tttttgctct gttaaagaaa tacctaattt ttcgaatgtt ttaagtaatt
     4081 cgggatctac ctcactgaga tcttttaatt ttttctgtga ttttggagcg gagtaatata
     4141 taacgtcttg gtaatcaatt tgtggaaatt ttatatatgc ccattcgggt tcaggcattt
     4201 gtttccattt cttataagct ttcaaacgaa aatttaataa aaattttgtt tctttcttct
     4261 tttgtgaaat taaatgaata gtcttttcat ttaaaccttt ttcaataata tctttttcaa
     4321 tggttgttga aaatccatat tgataagtct gatttaccaa attggttata tttttgttta
     4381 aaactttatt cgatttgtta accataatta attcgctaat ttaaaacata tagatgaatt
     4441 attcaaaatt ttttaactat tacttattat cattacggca ttataaagta atttttaact
     4501 gatttaacaa acttctaaaa ctttcaatta aaagctattc taaaaagtta taactttgtt
     4561 aaataatata tgtttttata atctatgtta aggatgttat aatgttagtt aaggttgtta
     4621 agattaactc aaccgacaga actaattttt taaaattagt taaatatcta ctatatattg
     4681 cctttttatt ccaaggagaa atcaatgtct caatctgtat cagaacggac tcgaattaaa
     4741 agtgaccgtt acgaatctgg tgtaattcct tatgctaaga tgggttactg ggatgctgct
     4801 tatgcagtaa aaaacactga tgttttagca ttattccgta tcacaccaca accaggtgta
     4861 gatccagtag aagctgctgc agctgtagct ggtgaatcat ctactgcaac atggactgtt
     4921 gtatggactg atttattaac agcttgtgat cgttaccgtg ctaaagctta ccgtgtagat
     4981 ccagttccaa atacaactga tcaatacttc gcttttatcg catatgaatg tgatttattt
     5041 gaagaaggtt ctttagctaa cttaacagct tctattattg gtaacgtatt cggtttcaaa
     5101 gctgtatctg cgttacgttt agaagatatg cgtatccctc attcttactt aaaaacgttc
     5161 caaggtcctg ctactggtgt aattgtagaa cgtgaacgtt taaacaaata tggtattcca
     5221 ttattaggtg ctacagtaaa acctaaatta ggtttatctg gtaaaaacta tggtcgtgta
     5281 gtttatgaag gtttaaaagg tggtttagac ttcttaaaag atgatgagaa tattaactca
     5341 caacccttca tgcgttggag agaacgtttc ttatactgta tggaaggtat taatcgcgct
     5401 tctgcagcta caggtgaaac taaaggttca tacctaaaca tcacagctgg tactatggaa
     5461 gaagtttaca aacgtgcaga atatgctaaa acagtaggtt ctatcgttgt tatgatcgat
     5521 ttagttatgg gttatactgc aatccaatca gctgcgattt gggcgcgtga taatgattta
     5581 attttacatt tacaccgtgc tggtaactca acttacgcgc gtcaaaaaaa tcatggtatc
     5641 aacttccgtg ttatttgtaa atggatgcgt atgtgtggtg tggatcatat ccatgctggt
     5701 acagttgtag gtaaattaga aggcgatcct ttaatgatta aaggtttcta cgatacttta
     5761 ttactaactc atttaaatgt taatttacca tacggtattt tcttcgaaat gacttgggca
     5821 agtttacgta aatgtatgcc tgttgcttct ggtggtatcc actgtggtca aatgcaccaa
     5881 ttagttcatt atttaggtga tgatgtagta ttacaattcg gtggtggtac aattggtcat
     5941 ccagatggta ttcaagctgg tgctacagct aatcgtgttg cgttagaagc aatgatttta
     6001 gctcgtaacg aaggtgctga ttactttaat agtgatattg gtcctcaaat tttacgcaac
     6061 gctgcgaaaa catgtggtcc tttacaaaca gctttagatt tatggaaaga tattagtttt
     6121 aactatacat ctacagatac atctgatttc tctgttacac caacagcaaa cgtataataa
     6181 attactttta aaaattaaag gagtatttga atagtgagac ttacacaagg ttgcttctcg
     6241 ttcttaccag atttaactga tcaacaaatt gaaaaacaaa tcgcttattg cattacaaaa
     6301 ggttgggcaa tgaacgttga gtggacagat gatccacatc cccgtaacag ttactgggaa
     6361 ttatggggtt tacctttatt tgatgttaaa gatcctgcat ctgtaatgtt tgaattaaga
     6421 gaagctcgta aatcatgcgc agctggttat atccgtatta atgcatttaa cgctgcttac
     6481 ggtactgaaa gttgtgttat gtctttcatt gtaaaccgtc ctagcaacga accaggtttc
     6541 tacttagaac gtcaagaact tgaaggtcgt cgtattgctt atactactaa aagttatagt
     6601 gttcaagcta atccagaagg tggtcgttac taatttttag taacaaaata aaattaaaaa
     6661 atgttaattg tttaaaacaa ttaacatttt ttacagtttt taatatgcca attctttttt
     6721 atttatattt ttttcaacaa atgaaatttg ttgatacaat cagtagtatc atttattaaa
     6781 caaactataa aataatgaat caaacgataa gctaaaattg agtttacaat accaacttta
     6841 gcttatcaca atgacaaaaa atgttaatct atttcaccta atacataacg tgtaaaacgt
     6901 tgtactcgaa tattttcacc caaaatacta actacctgtt taatatattc ggttacagta
     6961 atattaggat cacgaatata ctcttgttct aataaaactt gctttttcaa gcttttttca
     7021 actcggcctt ctacaatttt atttttaatt tcttcgggtt tattttgtaa atcatcttta
     7081 gcattttcaa atttccgcac ttctattttt gtcaaatcag aaatatcgtc taatgaaacg
     7141 acttgaattt ccggattaga cgcaatctgc atactaatat tttttgctaa gtcagtgaat
     7201 tcttcacgac gtgctacaaa atctgtctca caattaatct caagtaagat tcctaattta
     7261 cttcctgtgt gaatataaga ataaattaaa ccttcattcg tattacgact tgatttttta
     7321 tcagcagttg ccataccttt caatcttaat gattcgattg ccttatcata atcaccatga
     7381 ttttcagtta acgcttgttt acaagtcatc atacctgcac cggtttcatc acgcaatttt
     7441 tttactgttt tagcatcgat ttctgttttc atataatttt ataaaaattg tttataagtt
     7501 aattaaattt tctttacgac ttcccaataa gaatactatc tgttagagat tttagaatta
     7561 gtttaataga tcttaccgca tcatcatttc ctggaattgg aatatcgact aaatctggat
     7621 cacagtttgt atctaaaatt gatacaacag gaattcctaa ctttctacac tcacgaattg
     7681 ccgttgtttc tcgtttttgg tcaataacga tagcaatgtc tggtaagttt ttcatatctt
     7741 taataccatc taagtgccta cgtaattttt ttaattcttt tcgacgtaaa gcaccttctt
     7801 tctttgttaa taaatcgaaa gtattattag cttcttgttg ttcaagatct tttaaatgag
     7861 caatacgttc ttttaaagtt gaccaatttg ttagcattcc acccagccaa cgatgattta
     7921 cataatacga atcgcagcgt tttgcttcct gagcaattaa ggtgcttgct tgacgttttg
     7981 taccaacaaa taaaaatgtc ttattttcac gcgccgctga ttctagatat aaatttgctt
     8041 cttttaatag cgtagcagac tgtactaaat ctaaaatatg aatattattg acttcactat
     8101 aaatgtaagg gaacatcttt gggttccagc gataggcttt atggccaaag tggacgccgg
     8161 cttctaataa ttgagataaa cttatatcag acataaaatt ttaagttact taattaaata
     8221 tattaatttc tagcataaac cctagcagac aataaaaaac ttgtctagct ttctttctta
     8281 acgagtgaga atagtatcaa aagattttgg caattttgat ctaaccaaac tttctttttt
     8341 gaaacaattt cgataatttt gtgaaccagt tccagcggga attaaatgtc caataataat
     8401 gttttctttc aatccacgta accaatcaat acgtccttcg atagcggctt ttgttaatac
     8461 tctagtagtt tcctgaaaac tggcagcaga tataaaacta ggattattta acgctgcttt
     8521 tgtaatacca agaagtaatg gtacataaca agcggattgt tttccctggc tttgaataat
     8581 ttgatttata tactgaatat gatataagtc tataacttca cgtcttaaaa gtggagtatt
     8641 cccttcataa gtaatcaaaa cttttgtcgt catttgttta ataataactt ctaaatgttt
     8701 atcattaatg ataacacctt gagattgata aaccgcttgc acagaatcta aaataaaaga
     8761 ttgaactttt ttaaaacttt tataactacc ttcataattt tttattaaac gagcatattt
     8821 tatctcttct ttttgatcgc aaataaatgg tttaatccat ccataataat taaaataaac
     8881 ttttaacaat ttatggggat taactttttc attttcttta atattagttc caagcttacg
     8941 aaattcaaaa ttagtatcta aactcgattt ctgtactaat aaaccttttt tttgactcgt
     9001 cggaatgcgt tttatatttg aatttttttt ccgagcttcc aaaatctctt cgattcttgg
     9061 taaaccttgt acaatatcgc ctgtaatttc tttttctaaa tttaatagtc ctaacgtttc
     9121 cccttcttca ataaactcac tatttttaca aaacacactg ttaacatctt gttcaaaata
     9181 atcttctgtt attgagtata aaccaataga tttggttgat ttcgtgaaag gatactcaac
     9241 aaatttcagt agtgctaatt gataattaga attttttaaa tttttataat cgtttttcgt
     9301 aatctcagaa ctttgcatta ataatgttct gtccatttta ccaattgtac gttttgctaa
     9361 tccattaggt tgaattattt gtttgtactt tggactaaat tctgaagtgt gtaatgaaaa
     9421 ttttttgaaa aattggggta ttaatgaact atataatttt ccatttttct ttaaaaatag
     9481 ttttgaaaat tccgatttaa tcttaatggg atcctcgttt ttttcactga gagtacaatc
     9541 tttatttact gataatttca tcatatcata ataatttaat gaaattttgt aattaatatt
     9601 tttttccaga cgtggagaaa cacgagttgg agaaatagtt ttatattgta gatttgtctt
     9661 attaatcacg ttatcatctt tacaatttgg aaaaaaatac ggttttccct tttgtaatgt
     9721 taagttattt tcattttcaa taattatttt gcccgtttca ttcacgtttg aactattaat
     9781 aataaaatca tttaattttt taccaataaa cttctctttt ttcacagtta gacaatcttg
     9841 atttgaaatt aaaaggatct gtttactatc tttaattttt aattttattt ttacaatttc
     9901 taaagaattg gacgtttttg attctaaata acctaaatta gtataacgat ctataaattg
     9961 attgggttga attaataagc aagattgaag atttttatat cgtaaattag gtgaaatata
    10021 atgatttaaa ttaaattttt cgctaatttt aagatcgatt aatttggttt tttgattatg
    10081 tgatagctcc aaatttagat tttttgtgac agtttcttta gatttaagag tcaagatagt
    10141 cgaaattaga tttaaatttc gtgttccttt aatcaactgg tttggtttat atgaatatat
    10201 gatttttgag cttaatctaa tatttgagtc tttattatgc gtattttgaa ttttggcaga
    10261 aatattattt agatatggaa attcataaat ttcaattggt cgtacaagta attgctcaat
    10321 attttttcca gtaatatgtt cgcagaaaga aattttagta attggaatat gagaaaaaat
    10381 aatttcacct ggatagtata cttttttaga agtcatttta aacttttttc cttcataaac
    10441 taaaccagat ttaattgaaa ttgtgtgaat gagattattt ttttgtttaa ttacaacaac
    10501 tcccgaagtt ttggagaaaa gaccaggaat aagttcaaaa ccttcagaaa taaaatcgcc
    10561 atgttcagca agtaaaatat ttaattcaca atttacttta taaacttctt cacctaacca
    10621 gataatagtt ttatgagaaa caacaggttt ataattgttc aagaagttaa cacgacttaa
    10681 ataattaata gttgaatttg gtttgtaatt tttatataca ttttggtgat catagtaaac
    10741 tgttccaccc actaatgttt taaacctgaa gagcgggctt cgccctgtcg ctcgactgcg
    10801 gcgagcacta ctggctgtaa aaggacagac cacatcatgg ttctgtgttc attaggttgt
    10861 tctgtccatt gctgacataa tccgctccac ttcaacgtaa caccgcacga agatttctat
    10921 tgttcctgaa ggcatattca aatcgttttc gttaccgctt gcaggcatca tgacagaaca
    10981 ctacttccta taaacgctac acaggctcct gagattaata atgcggatct ctacgataat
    11041 gggagatttt cccgactgtt tcgttcgctt ctcagtggat aacagccagc ttctctgttt
    11101 aacagacaaa aacagcatat ccactcagtt ccacatttcc atataaaggc caaggcattt
    11161 attctcagga taattgtttc agcatcgcaa ccgtatcaga ctccggcatc gcaaactgca
    11221 cccggtgccg ggcagccaca tccagcgcaa aaaccttcgt gtagacttcc gttgaactga
    11281 tggacttatg tcccatcagg ctttgcagaa ctttcagcgg tataccggca tacagcatgt
    11341 gcatcgcata ggaatggcgg aacgtatgtg gtgtgaccgg aacagagaac gtcacaccgt
    11401 cagcagcagc ggcggcaacc gcctccccaa tccaggtcct gaccgttctg tccgtcactt
    11461 cccagatccg cgctttctct gtccttcctg tgcgacggtt acgccgctcc atgagcttat
    11521 cgcgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct
    11581 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta
    11641 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
    11701 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
    11761 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
    11821 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
    11881 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
    11941 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
    12001 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
    12061 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
    12121 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
    12181 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
    12241 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
    12301 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
    12361 tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa
    12421 acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgatg
    12481 ataagctgtc aaacatgaga attggtcgac ggcccgggcg gccgcaaggg gttcgcgttg
    12541 gccgattcat taatgcagct ggcacgacag gtttcccgac tagaaagcgg gcagtgagcg
    12601 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    12661 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacatag gaaacagcta
    12721 tgaccatgat tacgccaagc tatttaggtg agactataga atactcaagc ttgcatgcct
    12781 gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttcgccctat agtgagtcgt
    12841 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
    12901 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
    12961 gcaccgatcg cccttcccaa cagttgcgca gctgaatggc gaatggcgcc tgatgcggta
    13021 ttttctcctt acgcatctgt gtggtatttc acaccgcata tggtgcactc tcagtacaat
    13081 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgaa
    13141 ccccttgcgg ccgcatcgaa tataacttcg tataatgtat gctatacgaa gttattagcg
    13201 atgagctcgg acttccattg ttcattccac ggacaaaaac agagaaagga aacgacagag
    13261 gccaaaaagc tcgctttcag cacctgtcgt ttcctttctt ttcagagggt attttaaata
    13321 aaaacattaa gttatgacga agaagaacgg aaacgcctta aaccggaaaa ttttcataaa
    13381 tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg atcaccggaa aggacccgta
    13441 aagtgataat gattatcatc tacatatcac aacgtgcgtg gaggccatca aaccacgtca
    13501 aataatcaat tatgacgcag gtatcgtatt aattgatctg catcaactta acgtaaaaac
    13561 aacttcagac aatacaaatc agcgacactg aatacggggc aacctcatgt ccgagctcgc
    13621 gagctcgtcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg ccggcacggc
    13681 ctgggtaacc aggtattttg tccacataac cgtgcgcaaa atgttgtgga taagcaggac
    13741 acagcagcaa tccacagcag gcatacaacc gcacaccgag gttactccgt tctacaggtt
    13801 acgacgacat gtcaatactt gcccttgaca ggcattgatg gaatcgtagt ctcacgctga
    13861 tagtctgatc gacaatacaa gtgggaccgt ggtcccagac cgataatcag accgacaaca
    13921 cgagtgggat cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtc
    13981 ccagactaat aatcagaccg acgatacgag tgggaccgtg gttccagact aataatcaga
    14041 ccgacgatac gagtgggacc gtggtcccag actaataatc agaccgacga tacgagtggg
    14101 accatggtcc cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagtctg
    14161 attatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat
    14221 acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt
    14281 tccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagac taataatcag
    14341 accgacgata cgagtgggac catggtccca gactaataat cataccgacg atacgagtgg
    14401 gaccgtggtc ccagtctgat tatcagaccg acgatacgag tgggaccgtg gtcccagact
    14461 aataatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc agaccgacga
    14521 tacgagtggg accgtggtcc cagtctgatt atcagaccga cgatacaagt ggaacagtgg
    14581 gcccagagag aatattcagg ccagttatgc tttctggcct gtaacaaagg acattaagta
    14641 aagacagata aacgtagact aaaacgaggt cgcatcaggg tgctggcttt tcaagttcct
    14701 taagaatggc ctcaattttc tctatacact cagttggaac acgagacctg tccaggttaa
    14761 gcaccatttt atcgccctta tacaatactg tcgctccagg agcaaactga tgtcgtgagc
    14821 ttaaactagt tcttgatgca gatgacgttt taagcacaga agttaaaaga gtgataactt
    14881 cttcagcttc aaatatcacc ccagcttttt tctgctcatg aaggttagat gcctgctgct
    14941 taagtaattc ctctttatct gtaaaggctt tttgaagtgc atcacctgac cgggcagata
    15001 gttcaccggg gtgagaaaaa agagcaacaa ctgatttagg caatttggcg gtgttgatac
    15061 agcgggtaat aatcttacgt gaaatatttt ccgcatcagc cagcgcagaa atatttccag
    15121 caaattcatt ctgcaatcgg cttgcataac gctgaccacg ttcataagca cttgttgggc
    15181 gataatcgtt acccaatctg gataatgcag ccatctgctc atcatccagc tcgccaacca
    15241 gaacacgata atcactttcg gtaagtgcag cagctttacg acggcgactc ccatcggcaa
    15301 tttctatgac accagatact cttcgaccga acgccggtgt ctgttgacca gtcagtagaa
    15361 aagaagggat gagatcatcc agtgcgtcct cagtaagcag ctcctggtca cgttcattac
    15421 ctgaccatac ccgagaggtc ttctcaacac tatcaccccg gagcacttca agagtaaact
    15481 tcacatcccg accacataca ggcaaagtaa tggcattacc gcgagccatt actcctacgc
    15541 gcgcaattaa cgaatccacc atcggggcag ctggtgtcga taacgaagta tcttcaaccg
    15601 gttgagtatt gagcgtatgt tttggaataa caggcgcacg cttcattatc taatctccca
    15661 gcgtggttta atcagacgat cgaaaatttc attgcagaca ggttcccaaa tagaaagagc
    15721 atttctccag gcaccagttg atgagcgttg atcaatggcc tgttcaaaaa cagttctcat
    15781 ccggatctga cctttaccaa cttcatccgt ttcacgtaca acatttttta gaaccatgct
    15841 tccccaggca tcccgaattt gctcctccat ccacggggac tgagagccat tgctattgct
    15901 gtatttggta agcaaaatac gtacatcagg ctcgaaccct ttaagatcaa cgttcttgag
    15961 cagatcacga agcatatcga aaaactgcag tgcggaggtg tagtcaaaca actcagcagg
    16021 cgtgggaaca atcagcacat cagcagcaca tacgacatta atcgtgccga tacccaggtt
    16081 aggcgcgctg tcaataacta tgacatcata gtcatgagca acagtttcaa tggccagtcg
    16141 gagcatcagg tgtggatcgg tgggcagttt accttcatca aatttgccca ttaactcagt
    16201 ttcaatacgg tgcagagcca gacaggaagg aataatgtca agccccggcc agcaagtggg
    16261 ctttattgca taagtgacat cgtccttttc cccaagatag aaaggcagga gagtgtcttc
    16321 tgcatgaata tgaagatctg gtacccatcc gtgatacatt gaggctgttc cctgggggtc
    16381 gttaccttcc acgagcaaaa cacgtagccc cttcagagcc agatcctgag caagatgaac
    16441 agaaactgag gttttgtaaa cgccaccttt atgggcagca accccgatca ccggtggaaa
    16501 tacgtcttca gcacgtcgca atcgcgtacc aaacacatca cgcatatgat taatttgttc
    16561 aattgtataa ccaacacgtt gctcaacccg tcctcgaatt tccatatccg ggtgcggtag
    16621 tcgccctgct ttctcggcat ctctgatagc ctgagaagaa accccaacta aatccgctgc
    16681 ttcacctatt ctccagcgcc gggttatttt cctcgcttcc gggctgtcat cattaaactg
    16741 tgcaatggcg atagccttcg tcatttcatg accagcgttt atgcactggt taagtgtttc
    16801 catgagtttc attctgaaca tcctttaatc attgctttgc gtttttttat taaatcttgc
    16861 aatttactgc aaagcaacaa caaaatcgca aagtcatcaa aaaaccgcaa agttgtttaa
    16921 aataagagca acactacaaa aggagataag aagcgcacat acctcagtca cttattatca
    16981 ctagcgtcaa gtccagactc ctgtgtaaaa tgatttataa gggattttgc cgatttcggc
    17041 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    17101 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
    17161 cgcatagatc ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat
    17221 ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc
    17281 acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt
    17341 tctggcgtca gtccaccagc taacataaaa tgtaagcttt cggggctctc ttgccttcca
    17401 acccagtcag aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca
    17461 aacaagggaa taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct
    17521 tttggaaata cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac
    17581 gactcatctc catgcagttg gacgatatca atgccgtaat cattgaccag agccaaaaca
    17641 tcctccttag gttgattacg aaacacgcca accaagtatt tcggagtgcc tgaactattt
    17701 ttatatgctt ttacaagact tgaaattttc cttgcaataa ccgggtcaat tgttctcttt
    17761 ctattgggca cacatataat acccagcaag tcagcatcgg aatctagagc acattctgcg
    17821 gcctctgtgc tctgcaagcc gcaaactttc accaatggac cagaactacc tgtgaaatta
    17881 ataacagaca tactccaagc tgcctttgtg tgcttaatca cgtatactca cgtgctcaat
    17941 agtcaccaat gccctccctc ttggccctct ccttttcttt tttcgaccga attaattctt
    18001 aatcggcaaa aaaagaaaag ctccggatca agattgtacg taaggtgaca agctattttt
    18061 caataaagaa tatcttccac tactgccatc tggatcgctt gcctgtaact tacacgcgcc
    18121 tcgtatcttt taatgatgga ataatttggg aatttactct gtgtttattt atttttatgt
    18181 tttgtatttg gattttagaa agtaaataaa gaaggtagaa gagttacgga atgaagaaaa
    18241 aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt actttacata tatatttatt
    18301 agacaagaaa agcagattaa atagatatac attcgattaa cgataagtaa aatgtaaaat
    18361 cacaggattt tcgtgtgtgg tcttctacac agacaagatg aaacaattcg gcattaatac
    18421 ctgagagcag gtagagcaag gtaaaaggta gtatttgttg gcgatccccc tagagtcttt
    18481 tacatcttcg gaaaacaaaa actatttttt ctttaatttc tttttttact ttctattttt
    18541 aatttatata tttatattaa aaaatttaaa ttataattat ttttatagca cgtgatgctc
    18601 gccgcagccg tgtaaccgag catagcgagc gaactggcga ggaagcaaag aagaactgtt
    18661 ctgtcagata gctcttacgc tcagcgcaag aagaaatatc caccgtggga aaaactccag
    18721 gtagaggtac acacgcggat agccaattca gagtaataaa ctgtgataat caaccctcat
    18781 caatgatgac gaactaaccc ccgatatcag gtcacatgac gaagggaaag agaaggaaat
    18841 caactgtgac aaactgccct caaatttggc ttccttaaaa attacagttc aaaaagtatg
    18901 agaaaatcca tgcaggctga aggaaacagc aaaactgtga caaattaccc tcagtaggtc
    18961 agaacaaatg tgacgaacca ccctcaaatc tgtgacagat aaccctcaga ctatcctgtc
    19021 gtcatggaag tgatatcgcg gaaggaaaat acgatatgag tcgtctggcg gcctttcttt
    19081 ttctcaatgt atgagaggcg cattggagtt ctgctgttga tctcattaac acagacctgc
    19141 aggaagcggc ggcggaagtc aggcatacgc tggtaacttt gaggcagctg gtaacgctct
    19201 atgatccagt cgattttcag agagacgatg cctgagccat ccggcttacg atactgacac
    19261 agggattcgt ataaacgcat ggcatacgga ttggtgattt cttttgtttc actaagccga
    19321 aactgcgtaa accggttctg taacccgata aagaagggaa tgagatatgg gttgatatgt
    19381 acactgtaaa gccctctgga tggactgtgc gcacgtttga taaaccaagg aaaagattca
    19441 tagccttttt catcgccggc atcctcttca gggcgataaa aaaccacttc cttccccgcg
    19501 aaactcttca atgcctgccg tatatcctta ctggcttccg cagaggtcaa tccgaatatt
    19561 tcagcatatt tagcaacatg gatctcgcag ataccgtcat gttcctgtag ggtgccatca
    19621 gattttctga tctggtcaac gaacagatac agcatacgtt tttgatcccg ggagagacta
    19681 tatgccgcct cagtgaggtc gtttgactgg acgattcgcg ggctattttt acgtttcttg
    19741 tgattgataa ccgctgtttc cgccatgaca gatccatgtg aagtgtgaca agtttttaga
    19801 ttgtcacact aaataaaaaa gagtcaataa gcagggataa ctttgtgaaa aaacagcttc
    19861 ttctgagggc aatttgtcac agggttaatg gcaatttgtc acagacagga ctgtcatttg
    19921 agggtgattt gtcacactga aagggcaatt tgtcacaaca ccttctctag aaccagcatg
    19981 gataaaggcc tacaaggcgc tctaaaaaag aagatctaaa aactataaaa aaaataatta
    20041 taaaaatatc cccgtggata agtggataac cccaagggaa gttttttcag gcatcgtgtg
    20101 taagcagaat atataagtgc tgttccctgg tgcttcctcg ctcactcgac cgggagggtt
    20161 cgagaagggt gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
    20221 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
    20281 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
    20341 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
    20401 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
    20461 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
    20521 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
    20581 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    20641 aagttttccg cgaggtatcc acaacgccgg cggccggccg cggtgtctcg cacacggctt
    20701 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
    20761 aaccaggctc ttcaccatgt gttgttccaa cgagttgaac gcctttttct gcaattgttc
    20821 tagcagctaa gacttcaagt tctgtaccaa tttcatcaat gattataact tgtggcatat
    20881 ggttttctac agcttcaatc ataacttgat gctgtaattc agtcttagca acttgcattc
    20941 tccttgctct gccaataccg gaatgtggta tatcactatc accagcaatt tcattagaag
    21001 tgtcaataat tatgactctt ttctccattt catcagctaa gacccgagca atttcacgaa
    21061 tgatagtggt tttacctact cctggtttac ctaagattaa aatagacttt tctgattcta
    21121 ataaatctcg aatagcagaa attgtcccaa atacagctct gccaactcga caagttaatc
    21181 catttattaa aaattgccta tttctaatac agctaattcg gtgaagagtt ctttcgatac
    21241 ccgctcgatt ttcattacta aatttactta ctcttttcgt catataatca atatcttgcc
    21301 aagaaataat tttttgagac aagtattgag gaccagtaat gaaacgagct tcgggacgtc
    21361 ttcctaaatc taatacaatt tcaataagtt gatctttcga atcatgttga tttaagtgtt
    21421 cacgaagaaa tgcgggtaga ttttcaatta atttgtctaa atctccatta acactcatct
    21481 ttgtctaaat ttaatatagt ttttaattta gaatacagtt ttaaaatgaa cacaatttta
    21541 atttaattct ttttgttaag catttagtaa ggttttaaaa gccactgtat ctaatacagc
    21601 aatttgtgat aacattttac gatttaaatc aatatttgat tttttaaatc tatgaattaa
    21661 ttgactatag gaaatgccat ttaaacgcga agcagcgtta attcgactaa tccaaattct
    21721 tcgaaatatt cgtttttttt gttttcggcc gacgtaagaa taacgtaaag ctttcattac
    21781 ttgttgattt gcaactctaa ataaaactga gtgcgcgcct ctataaccac ttgcaagtgc
    21841 taatattttt ttacgacgtt tacgagcgac attaccgcgt ttgactctta ccatgttttt
    21901 ttctgattaa tatattaata aggtaacatt aatttgattg attttatatc acttctactt
    21961 acacaaatag tttgcgacaa tttccgtttt tgtttatttg attttttcat taaaagatgt
    22021 cctttaaaag cgtgtcgccg taagaaatta ctagtacccg ttcttttata tcgtttcgct
    22081 gctgcttttc gagtctttaa ttttggcata attttttaag tctttttttg agaatataaa
    22141 gttttgaaat aaagctttga tttcaacgga tgagagtata aaattttgtc cccatatttc
    22201 caatcaaccg ggcacgcttg tcccggattt tctttaagat actgaagaga ttgtaaaata
    22261 cgaagtaact cattgacact tcgtccacat aataagttat taaccgtata gtattgaact
    22321 ataccttctt tgtcaataat aaatacacct ggtaaagaga atccatcttt tgttaataat
    22381 ttataattac ttgtaattgt ctgacttaaa tctgaaatta agggatagtt taagtgctca
    22441 agtccaccct gatttctctt tgataataaa aattgtaaat gcgaaaatgg gctatctaca
    22501 gagattgcaa gaatttgtgt agatagtttt ttaaattcag taattcgatt atttaattct
    22561 actaactctg ttgatgaaac agcggtaaaa ttggatggat aaaatattaa gactacatat
    22621 ttttttccat gataatctga tagtcgaatt ttacccaatt gattcttata aacaccaact
    22681 ccaataaaat taggcgccat ttgaccaatt tgaatatgtt ctaacataat aaatgactat
    22741 ttaaaaataa aaatttcaaa tacaaaacaa tactataata aaaaaagtat agcaatcaat
    22801 caaagattac taaacttttt aatcgttaat gtctctgaat gattgttact tatccttttt
    22861 ttttggactg ctaacttcaa ctaactcact taatgcaaaa ttattcgtat ttgtgccact
    22921 gtaattaact ttttcaaatc ttacaacggc tgggtaacga attccactct gatctacggt
    22981 tgcaacagtt ccgacttcgt taaaccaata tgactcttct cttaaaattc gaacttctga
    23041 accacgatca atcataatta aaattttaat aaattatagt attggatctt attctaagtc
    23101 attccttaac aattttaaca ataaaaattt aatgatagtt ggtttaagag agccaattgt
    23161 gttattgttt agttaagaac aaaactataa ataatatata tatattaatt aacaaatata
    23221 atggaacgaa atacaataca aaaaattctt attggattat ttctcttggc atgtattttc
    23281 attggcttaa gaacctttaa tgtaattggc tttgaagaag tacagtcaaa acctgaagtt
    23341 agtgccaatg taagtagttc aagaatgact tatggtcgat ttttagaata tttagaaatg
    23401 ggatgggtaa aacaagttga cttatatgat aatagtcgaa atgcaattgt tcaagcttcg
    23461 agcccagaac ttggaaatcg tccgcaatcg attagagtcg aaattccagt tggaacatct
    23521 caattaatac aaaaattaaa agaatatgaa atcaattttg atgctcatcc agcacccaaa
    23581 aaaagtattt ttattacatt agcaagtaat ttattattac cattaatctt tattggaagt
    23641 ttaattttct tttttcaaaa ttcggacaat ctatctggaa attctaattc atcgccaatg
    23701 aatttaggta aatcaccagc gcgatttgat caacgtccag atacaggtat ctcatttgat
    23761 gatattgcag gtattgatga agcaaaggca gaatttgaag agatagtatc atttttaaaa
    23821 gaacctgagc gatatacttt agttggagca aaaataccaa aaggggtatt attagtagga
    23881 cctccaggaa cgggtaagac attattagca aaagcaattg ctaatgaagc taatgtacca
    23941 ttctatagtg ttgctggatc agagtttgta gaaatgttta ttggtattgg tgcatcacga
    24001 atacgtgatt tatttaaaaa ggcgtcagaa aatactccat gtattgtctt tatcgatgaa
    24061 attgatgctg taggtcgtga acgaggtgcc ggagttggtg gtggaaacga tgagcgagaa
    24121 caaactttaa atcaattatt aacagaaatg gatggattta aagaaaataa aggggtcatt
    24181 gttgttggtg caaccaatcg agtggatatt ttagatgctg cattattacg tccaggccga
    24241 tttgatcgcc aaattactgt tggtctacca gatcgtttag gtagaattgg tattctgaaa
    24301 gttcacgcta agaataagcc atttgccgaa gatgtatccc tagttcaatt agctaatcgt
    24361 acaccaggat tttcgggcgc agatttggct aatttattaa acgaagcagc tattttagcg
    24421 acacgttata aaaaagtaac gatcacaaaa aatgaagtta atgaagcagc tgatagaatt
    24481 attggtggta tcgctggtag tacaatggaa gatactaaaa ataaaaaatt aattgcttat
    24541 catgaagttg gtcatgcaat tgtcggatcg gtattagaaa atcatgatga agttgaaaag
    24601 attactttag ttccacgcgg cggggcaaaa ggtcttacat ggtttgcacc agaagaagat
    24661 caaatgttac tttcgcgatc agctttattg gctcgaatta ttacaacgtt agcaggtcgg
    24721 gctgcagaac aagtcgtttt tggtgatcca gaaattacga ctggtgcaag taatgatcta
    24781 caacaagtta caaatattgc ccgccagatg gtaactcgtt acggtatgtc aaatattgga
    24841 ccaattgcct tagaagatga taataatcaa caaatgttca tgggtggcga atataacgaa
    24901 gcaatcgctg atagaattga ttctgaggtt tgtaaaatta ttaatcattg tgaaaagata
    24961 gcaatagaaa taattcgaga taatcgtgtg gtgattgatc tagtcgtaga aaaattatta
    25021 gacgcagaaa caatagatgg attagaattc cgtaaattaa ttaatcaata cactgttttg
    25081 ccagtcaaaa attaatagaa aattgcccta aaaagctcaa cttcatttca gtttaaaaat
    25141 tgatctgaag ttgagctttt tagggcaatt ttaatgcttt aagataataa attttttatt
    25201 gttatatttt aactaagaaa acataaataa aaaaaattag gtctgataat atatttttgc
    25261 cactgaatag aatttagtca gtggcaaaaa tactaattag ttttactaaa agcttctttt
    25321 gattcaagaa ttgcttcttt taaagacgat tcagcctctt cggtaaattg gtttgttgta
    25381 cgaacaattt cggtgtatgg tttttttgaa gtagttaaat atgtcatcaa acttgcacaa
    25441 tacgatttaa catttgaaac cggtaaatca tctaaataac cattaattcc tgtataaatc
    25501 agagcaactt gttgagcaac agataacgga gagttttgtg gttgctttaa aacctcacgt
    25561 aagcgtgttc cacgtgctaa ctgtttttgt gttgcttcgt ctaagtctga agcaaattgc
    25621 gaaaatgcct ctaattctgc aaattgagct aattctaact tcaatttacc tgcaactttt
    25681 ttcattgctt tagtttgtgc agcagatccg acacgtgata ccgagatacc gacattaatc
    25741 gctgggcgaa tacctgagtt aaatagatca tttgataaga aaatttgccc atcggtaatt
    25801 gaaattacat tcgttggaat ataagctgat acgtcactag cttgtgtttc aataatcgga
    25861 agtgctgtca tacttccacc acctagttta tcactaagtt ttgcagcacg ttctagcaaa
    25921 cgtgagtgta agtaaaatac gtcacctgga tatgcctcac gtcctggtgg ccgacgcaac
    25981 aataatgaca tttgacggta agcaacggct tgttttgtta aatcatcata aacaattagt
    26041 gttgctttac ctttatacat aaaatattct gccaatgctg caccggcata tggtgctata
    26101 tattgtaatg ttgctggatc attggcacta gatgctacaa caattgtgta tgacattgca
    26161 tttttttctt caagcacgtt tacaacttga gcaatagttg aagctttttg gccaacacct
    26221 acgtaaacac aaattacatc ttcagtttgt tggttaataa tcgtatcaac tgcaatcgat
    26281 gttttaccag tttgacggtc accgataatt aactcacgtt gaccacgccc aattggaatc
    26341 attgcatcaa tcgatgtaat acccgtttgt aaaggttcac aaactgattt acgactaata
    26401 ataccaggtg ccatcgcttc aagtaatcga gaatctgatg ttacgatttc acctttacca
    26461 tcaattgcta cacctagagg gctaacaaca cgacctaaaa attcatctcc tacaggaatt
    26521 tgagcaattc taccagttgc tttaactgta ctaccctcta aaatttgacg tcccgtaccc
    26581 attagtacaa cgccaacgtt gtcgttttct aaatttaagg cgataccgat agttttatct
    26641 tcaaactcta ataattcacc actcattacc tgatcaagac cataaacacg ggcaatacca
    26701 tcaccaactt gtaatacagt accaatatta tctaccttaa catcttgatc atatttttca
    26761 atctgctcac ggataatact actaatttcg tctggacgaa tatttatcat tgtattattt
    26821 taaaataaaa agttaataaa agatttattt ataattaaat ttctaaaaca gtatctaagt
    26881 gttttgctaa tttttgtaat tgatttttta ttgtaaaatc tattaccttc gattgagtct
    26941 taattaaaaa acctccaatt aagttaggat caacggtaat cattaatcta atttcgcgag
    27001 cattcattaa ttcttttaat ttctgaacta agatcgtttt ttgcgaagtt gtgaactcaa
    27061 aagcagttga aacttcaatt gttttaattg atgctgtttc atatacaagt tctaaataaa
    27121 tcgtaataac tgacgttaat aagctaatac gtcctcgttc cactaaaatc attaaaaact
    27181 ttaaagtttc atcgttaagc tgcgcttgta atgtttttgc taaaatttca cattttgcat
    27241 tcttactaac aactggattg tctaaatatt cagttaattc agtattttct tttaaaaaaa
    27301 tttctaaatt ttgaaaatct gcggtaattt gatgcattat atttttttca actgaaaaat
    27361 caaataatgc tcgtgcgtat ggacttgcaa ttttcaacgt taatggattt acgctcatag
    27421 taaatctcct tccaatttat taatagtatc atttattaac gtagttgcac gttcttttgg
    27481 tgcaaatgct tgttgtgcac gaattaccgt ccgtttcagc acaagcgaaa taatttgttg
    27541 tttaatttct aaaaatattt gacgctcttt tgacttaaac attgctaatg cacgttcaaa
    27601 acgaattttt aaatcttttt tagctccaaa tacttctgat tctaataat
//
