LOCUS       sequence-420032-       10516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector to express FLAG-VSVG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pLV_CMV_FLAG-VSVG
FEATURES             Location/Qualifiers
     source          1..10516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(450..1241,1242..1310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1073..1092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1311..1415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1490..1509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1681..2060
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2062..2260
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2278..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2505..2630
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3123..3356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3541..3585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3883..4000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4035..4413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4414..4617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4662..4681
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4662..4680
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4783..4806
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6253..6285
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6301..6357
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7075..7131
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7132..7731
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7132..7151)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7628..7648
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7756..8344
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7809..7829)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8227..8238)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8347..8363)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8348..8364)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8869..9049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9075..9092)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9081..9305
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9351..9779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9438..9457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9648..9669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9805..9821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9805..9821)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9818..9840)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9829..9845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9853..9859,9860..9877,9878..9883))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9898..9919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10036..10053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10207..10516,1..279))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10287..10306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
       61 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1441 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1501 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1561 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1621 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     1681 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     1741 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     1801 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     1861 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     1921 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     1981 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2041 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2101 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2161 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2221 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     2281 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2341 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2401 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2461 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2521 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2581 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     2641 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     2701 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     2761 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     2821 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2881 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2941 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     3001 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3061 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3121 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     3181 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     3241 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     3301 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     3361 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     3421 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     3481 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     3541 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     3601 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     3661 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     3721 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     3781 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     3841 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     3901 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     3961 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4021 cagagatcca gtttacattg attattgact agttattaat agtaatcaat tacggggtca
     4081 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     4141 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     4201 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     4261 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     4321 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     4381 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     4441 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4501 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4561 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     4621 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
     4681 gagacccaag ctggctagcc caccatgaag tgccttttgt acttagcctt tttattcatt
     4741 ggggtgaatt gcaagttcac catagttttt ccatccggag gagattacaa ggatgacgac
     4801 gataagggcg gaagcttggg acacaaccaa aaaggaaact ggaaaaatgt tccttctaat
     4861 taccattatt gcccgtcaag ctcagattta aattggcata atgacttaat aggcacagcc
     4921 ttacaagtca aaatgcccaa gagtcacaag gctattcaag cagacggttg gatgtgtcat
     4981 gcttccaaat gggtcactac ttgtgatttc cgctggtatg gaccgaagta tataacacat
     5041 tccatccgat ccttcactcc atctgtagaa caatgcaagg aaagcattga acaaacgaaa
     5101 caaggaactt ggctgaatcc aggcttccct cctcaaagtt gtggatatgc aactgtgacg
     5161 gatgccgaag cagtgattgt ccaggtgact cctcaccatg tgctggttga tgaatacaca
     5221 ggagaatggg ttgattcaca gttcatcaac ggaaaatgca gcaattacat atgccccact
     5281 gtccataact ctacaacctg gcattctgac tataaggtca aagggctatg tgattctaac
     5341 ctcatttcca tggacatcac cttcttctca gaggacggag agctatcatc cctgggaaag
     5401 gagggcacag ggttcagaag taactacttt gcttatgaaa ctggaggcaa ggcctgcaaa
     5461 atgcaatact gcaagcattg gggagtcaga ctcccatcag gtgtctggtt cgagatggct
     5521 gataaggatc tctttgctgc agccagattc cctgaatgcc cagaagggtc aagtatctct
     5581 gctccatctc agacctcagt ggatgtaagt ctaattcagg acgttgagag gatcttggat
     5641 tattccctct gccaagaaac ctggagcaaa atcagagcgg gtcttccaat ctctccagtg
     5701 gatctcagct atcttgctcc taaaaaccca ggaaccggtc ctgctttcac cataatcaat
     5761 ggtaccctaa aatactttga gaccagatac atcagagtcg atattgctgc tccaatcctc
     5821 tcaagaatgg tcggaatgat cagtggaact accacagaaa gggaactgtg ggatgactgg
     5881 gcaccatatg aagacgtgga aattggaccc aatggagttc tgaggaccag ttcaggatat
     5941 aagtttcctt tatacatgat tggacatggt atgttggact ccgatcttca tcttagctca
     6001 aaggctcagg tgttcgaaca tcctcacatt caagacgctg cttcgcaact tcctgatgat
     6061 gagagtttat tttttggtga tactgggcta tccaaaaatc caatcgagct tgtagaaggt
     6121 tggttcagta gttggaaaag ctctattgcc tcttttttct ttatcatagg gttaatcatt
     6181 ggactattct tggttctccg agttggtatc catctttgca ttaaattaaa gcacaccaag
     6241 aaaagacaga tttatacaga catagagatg aaccgacttg gaaaggaatt cggatccggc
     6301 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
     6361 gtgtccaagg gcgaggaaga taacatggcc atcatcaagg agttcatgag gtttaaggtc
     6421 cacatggagg gttcagtcaa tggccacgag ttcgagattg aaggcgaggg cgagggccgc
     6481 ccctacgaag ggacacagac ggcgaaattg aaggtgacca aaggcgggcc attgcccttc
     6541 gcatgggaca tcttgtcccc tcagtttatg tatggcagca aggcctatgt taagcacccc
     6601 gctgatatcc cggactactt gaagctgtcc tttccagagg ggtttaaatg ggagcgcgtt
     6661 atgaatttcg aagacggagg agtggttacg gtgacgcagg actcatccct gcaggacgga
     6721 gaatttatat ataaggttaa gttgagaggc acaaacttcc caagcgacgg ccctgtgatg
     6781 cagaagaaaa caatggggtg ggaagcttcc agcgagcgca tgtaccccga agatggcgcc
     6841 ctcaagggcg agataaagca aaggctgaaa cttaaggacg gcggtcatta cgacgcggag
     6901 gtcaagacaa cttacaaggc taaaaaaccc gttcagttgc ctggggctta caatgttaat
     6961 atcaaacttg acatcacaag ccacaatgaa gactatacga tcgtggagca gtatgaacga
     7021 gcggaaggca ggcactcaac gggggggatg gacgagcttt acaagggatc cggcgcaaca
     7081 aacttctctc tgctcaaaca agccggcgac gtcgaagaga atcctgggcc gatgaccgag
     7141 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     7201 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     7261 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     7321 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7381 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7441 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7501 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7561 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7621 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7681 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta agaattcgat
     7741 atcaagctta tcggtaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7801 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7861 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7921 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7981 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8041 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8101 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8161 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8221 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8281 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8341 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     8401 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     8461 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8521 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     8581 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     8641 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     8701 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     8761 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     8821 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     8881 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8941 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9001 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     9061 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     9121 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     9181 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     9241 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     9301 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     9361 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     9421 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9481 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9541 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9601 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9661 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9721 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     9781 attctgtagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9841 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9901 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9961 gtcgtgtcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    10021 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    10081 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    10141 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10201 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10261 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    10321 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10381 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    10441 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10501 cggtaactat cgtctt
//
