LOCUS       sequence-420044-        9529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of W403A Parkin, internally 
            tagged with mKO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Waters CS, Angenent SB, Altschuler SJ, Wu LF
  TITLE     A PINK1 input threshold arises from positive feedback in the 
            PINK1/Parkin mitophagy decision circuit.
  JOURNAL   Cell Rep. 2023 Oct 31;42(10):113260. doi: 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17.
  PUBMED    37851575
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-10-31"; volume: "42(10):113260. doi"; pages: " 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17"
COMMENT     Sequence Label: pLv-EF1a-Parkin(W403A)-A92mKO2
FEATURES             Location/Qualifiers
     source          1..9529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        34..245
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          246..1184
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1141..1161
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1499..2149
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             1499..2149
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     misc_feature    3311..3899
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3364..3384)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3782..3793)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4106..4739
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4867..4883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4867..4883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4880..4902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4891..4907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4915..4921,4922..4939,4940..4945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4960..4981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5098..5115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5269..5857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5349..5368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6028..6819,6820..6888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6651..6670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6889..6993)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7041..7175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7078..7097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7132..7151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7343..7976
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8023..8148
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8645..8878
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9063..9107
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9370..9485
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gccctttcgt cttcactcga ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
       61 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
      121 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
      181 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
      241 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
      301 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
      361 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
      421 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
      481 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
      541 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
      601 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
      661 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
      721 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
      781 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
      841 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
      901 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
      961 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     1021 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     1081 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     1141 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggacgcg
     1201 tgccaccatg atagtgtttg tcaggttcaa ctccagccat ggtttcccag tggaggtcga
     1261 ttctgacacc agcatcttcc agctcaagga ggtggttgct aagcgacagg gggttccggc
     1321 tgaccagttg cgtgtgattt tcgcagggaa ggagctgagg aatgactgga ctgtgcagaa
     1381 ttgtgacctg gatcagcaga gcattgttca cattgtgcag agaccgtgga gaaaaggtca
     1441 agaaatgaat gcaactggag gcgacgaccc cagaaacgcg gcgggtgctg gttctggcgt
     1501 gagcgtgatc aagcccgaga tgaagatgag gtactacatg gacggctccg tcaatgggca
     1561 tgagttcaca atcgagggtg agggcacagg cagaccttac gagggacatc aggagatgac
     1621 actgcgcgtc acaatggccg agggcgggcc aatgcctttc gccttcgacc tggtgtccca
     1681 cgtgttctgt tacggccaca gagtttttac caagtaccca gaagagatcc cagactattt
     1741 caagcaggcc tttcctgagg gcctgtcctg ggagaggtcc ctggagttcg aggacggcgg
     1801 ctccgcctcc gtgagcgccc acatcagcct gaggggcaac accttctacc acaagtccaa
     1861 gttcaccggc gtgaacttcc ccgccgacgg ccccatcatg cagaaccaga gcgtggactg
     1921 ggagccctcc accgagaaga tcaccgccag cgacggcgtg ctgaagggcg acgtgaccat
     1981 gtacctgaag ctggagggcg gcggcaacca caagtgccag atgaagacca cctacaaggc
     2041 cgccaaggag atcctggaga tgcccggcga ccactacatc ggccacaggc tggtgaggaa
     2101 gaccgagggc aacatcaccg agcaggtgga ggacgccgtg gcccactcag caggatcagg
     2161 aggtggaggc tgtgagcggg agccccagag cttgactcgg gtggacctca gcagctcagt
     2221 cctcccagga gactctgtgg ggctggctgt cattctgcac actgacagca ggaaggactc
     2281 accaccagct ggaagtccag caggtagatc aatctacaac agcttttatg tgtattgcaa
     2341 aggcccctgt caaagagtgc agccgggaaa actcagggta cagtgcagca cctgcaggca
     2401 ggcaacgctc accttgaccc agggtccatc ttgctgggat gatgttttaa ttccaaaccg
     2461 gatgagtggt gaatgccaat ccccacactg ccctgggact agtgcagaat ttttctttaa
     2521 atgtggagca caccccacct ctgacaagga aacatcagta gctttgcacc tgatcgcaac
     2581 aaatagtcgg aacatcactt gcattacgtg cacagacgtc aggagccccg tcctggtttt
     2641 ccagtgcaac tcccgccacg tgatttgctt agactgtttc cacttatact gtgtgacaag
     2701 actcaatgat cggcagtttg tccacgaccc tcagcttggc tactccctgc cttgtgtggc
     2761 tggctgtccc aactccttga ttaaagagct ccatcacttc aggattctgg gagaagagca
     2821 gtacaaccgg taccagcagt atggtgcaga ggagtgtgtc ctgcagatgg ggggcgtgtt
     2881 atgcccccgc cctggctgtg gagcggggct gctgccggag cctgaccaga ggaaagtcac
     2941 ctgcgaaggg ggcaatggcc tgggctgtgg gtttgccttc tgccgggaat gtaaagaagc
     3001 gtaccatgaa ggggagtgca gtgccgtatt tgaagcctca ggaacaacta ctcaggccta
     3061 cagagtcgat gaaagagccg ccgagcaggc tcgtgcggaa gcagcctcca aagaaaccat
     3121 caagaaaacc accaagccct gtccccgctg ccatgtacca gtggaaaaaa atggaggctg
     3181 catgcacatg aagtgtccgc agccccagtg caggctcgag tggtgctgga actgtggctg
     3241 cgagtggaac cgcgtctgca tgggggacca ctggttcgac gtgtaagatc caatgcatcg
     3301 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3361 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3421 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3481 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3541 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3601 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3661 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     3721 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     3781 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     3841 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     3901 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     3961 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     4021 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     4081 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     4141 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     4201 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     4261 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     4321 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     4381 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     4441 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     4501 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     4561 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4621 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4681 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4741 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     4801 gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta
     4861 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4921 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4981 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5041 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5101 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5161 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5221 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5281 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5341 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5401 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5461 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5521 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5581 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5641 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5701 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5761 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5821 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5881 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5941 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6001 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6061 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6901 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6961 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7021 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
     7081 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7141 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     7201 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     7261 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     7321 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
     7381 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     7441 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     7501 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     7561 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     7621 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     7681 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     7741 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     7801 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7861 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7921 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7981 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     8041 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     8101 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     8161 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     8221 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     8281 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     8341 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     8401 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     8461 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     8521 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     8581 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     8641 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     8701 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     8761 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     8821 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     8881 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     8941 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     9001 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     9061 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     9121 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     9181 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     9241 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     9301 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     9361 ggtatcgcct ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     9421 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     9481 aattttcggg tttattacag ggacagcaga gatccagttt atcgatgag
//
