LOCUS       sequence-420107-        4245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PU_Zea mays Ubiquitin1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Lee DY, Acosta-Gamboa L, Saleh L, Pathak S, Swyers N, Morgan A, 
            Meerdink S, Kuzio C, Calderon S, Sheng H, Kenney S, Zare A, Gehan M,
            Nusinow DA
  TITLE     Remote Sensing of Endogenous Pigmentation by Inducible Synthetic 
            Circuits in Grasses.
  JOURNAL   Plant Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480.
  PUBMED    41351300
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480."
COMMENT     Sequence Label: pDL0_011
FEATURES             Location/Qualifiers
     source          1..4245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2238..4229
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaggagctg cagtgcagcg tgacccggtc gtgcccctct ctagagataa
     2281 tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt
     2341 gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga ataatataat
     2401 ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg
     2461 gtctaaagga caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg
     2521 catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat
     2581 tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat
     2641 ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt
     2701 atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc
     2761 ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc
     2821 ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc
     2881 gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag
     2941 ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg
     3001 cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg
     3061 gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca
     3121 ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca
     3181 gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc
     3241 cccccccctc tctaccttct ctagatcggc gttccggtcc atggttaggg cccggtagtt
     3301 ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt
     3361 cgtacacgga tgcgacctgt acgtcagaca cgttctgatt gctaacttgc cagtgtttct
     3421 ctttggggaa tcctgggatg gctctagccg ttccgcagac gggatcgatt tcatgatttt
     3481 ttttgtttcg ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc
     3541 acttgtttgt cgggtcatct tttcatgctt ttttttgtct tggttgtgat gatgtggtct
     3601 ggttgggcgg tcgttctaga tcggagtaga attctgtttc aaactacctg gtggatttat
     3661 taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg
     3721 atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac
     3781 agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc
     3841 gttctagatc ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac
     3901 tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc
     3961 taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca
     4021 gcatctattc atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt
     4081 ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt
     4141 tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct
     4201 caccctgttg tttggtgtta cttctgcaga atgtgagacc acgaa
//
