LOCUS       sequence-420119-        4045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mariner transposon with ATc-inducible himar1C9 transposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   English MA, Alcantar MA, Collins JJ
  TITLE     A self-propagating, barcoded transposon system for the dynamic 
            rewiring of genomic networks.
  JOURNAL   Mol Syst Biol. 2023 Jun 12;19(6):e11398. doi: 
            10.15252/msb.202211398. Epub 2023 Mar 27.
  PUBMED    36970845
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2023-06-12"; volume: "19(6):e11398. doi"; pages: " 
            10.15252/msb.202211398. Epub 2023 Mar 27"
COMMENT     Sequence Label: pDonor_Tn[himar1C9]
FEATURES             Location/Qualifiers
     source          1..4045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      51..439
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            554..663
                     /note="incP origin of transfer"
     primer_bind     843..861
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1187..1846
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1253..1272)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1917..1951
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1956..2582)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     2487..2506
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        2598..2653
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    2634..2652
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2671..2682
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3760..3846
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3938..3965
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctctagagta gtagtccatg ggtatggaca gcggtggagg ggaattccca tgtcagccgt
       61 taagtgttcc tgtgtcactc aaaattgctt tgagaggctc taagggcttc tcagtgcgtt
      121 acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat
      181 tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg atttatatta
      241 attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag tacgttagcc
      301 atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga gcttagtacg
      361 ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat caacaggttg
      421 aactgctgat cttcagatcc tctacgccgg acgcatcgtg gccggatcaa ttccgtgata
      481 ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt
      541 acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
      601 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
      661 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
      721 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
      781 ggttatgcag cgtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      841 aaaataggcg tatcacgagg ccctttcgtc acgcgtcctc ggtacctaac aggttggatg
      901 ataagtcccc ggtcttcgta tgccgtcttc tgcttggcgc gccctcgagc aattgccgac
      961 cgaattttta tgtcgtaaag aggggctttg cagggggtgg actcagaaag atgagaatag
     1021 atgactattg tagttgaaac acatagaaag ttgctgatat acagaccgat acgcatatcg
     1081 gttgatcggg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
     1141 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     1201 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     1261 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     1321 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     1381 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
     1441 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     1501 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     1561 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     1621 ttcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     1681 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     1741 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     1801 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttg atatcgagct
     1861 cgcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1921 gaacgctcgg ttgccgccgg gcgtttttta ttggtttaag acccactttc acatttaagt
     1981 tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg ctctgcacct
     2041 tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc agtagtaggt
     2101 gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca acctaaagta
     2161 aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc ttgttggcat
     2221 aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg tgtacctaaa
     2281 tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt agcgttatta
     2341 cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg gtgcctatct
     2401 aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg ccaatacaat
     2461 gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc ttcgattccg
     2521 acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa tctagacatc
     2581 attaattcct aatttttgtt gacactctat cgttgataga gttattttac cactccctat
     2641 cagtgataga gaaaagaatt caaaagatct aaagaggaga aaggatctat ggaaaaaaag
     2701 gaatttcgtg ttttgataaa atactgtttt ctgaagggaa aaaatacagt ggaagcaaaa
     2761 acttggcttg ataatgagtt tccggactct gccccgggga aatcaacaat aattgattgg
     2821 tatgcaaaat tcaagcgtgg tgaaatgagc acggaggacg gtgaacgcag tggacgcccg
     2881 aaagaggtgg ttaccgacga aaacatcaaa aaaatccaca aaatgatttt gaatgaccgt
     2941 aaaatgaagt tgatcgagat agcagaggcc ttaaagatat caaaggaacg tgttggtcat
     3001 atcattcatc aatatttgga tatgcggaag ctctgtgcga aatgggtgcc gcgcgagctc
     3061 acatttgacc aaaaacaacg acgtgttgat gattctaagc ggtgtttgca gctgttaact
     3121 cgtaatacac ccgagttttt ccgtcgatat gtgacaatgg atgaaacatg gctccatcac
     3181 tacactcctg agtccaatcg acagtcggct gagtggacag cgaccggtga accgtctccg
     3241 aagcgtggaa agactcaaaa gtccgctggc aaagtaatgg cctctgtttt ttgggatgcg
     3301 catggaataa tttttatcga ttatcttgag aagggaaaaa ccatcaacag tgactattat
     3361 atggcgttat tggagcgttt gaaggtcgaa atcgcggcaa aacggcccca catgaagaag
     3421 aaaaaagtgt tgttccacca agacaacgca ccgtgccaca agtcattgag aacgatggca
     3481 aaaattcatg aattgggctt cgaattgctt ccccacccgc cgtattctcc agatctggcc
     3541 cccagcgact ttttcttgtt ctcagacctc aaaaggatgc tcgcagggaa aaaatttggc
     3601 tgcaatgaag aggtgatcgc cgaaactgag gcctattttg aggcaaaacc gaaggagtac
     3661 taccaaaatg gtatcaaaaa attggaaggt cgttataatc gttgtatcgc tcttgaaggg
     3721 aactatgttg aataacgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
     3781 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     3841 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca
     3901 ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc
     3961 ctttttgcgt ttctacctgc agggcgcgcc aagcagaaga cggcatacga agaccgggga
     4021 cttatcatcc aacctgttag gatcc
//
