LOCUS       sequence-420144-        9529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of N273K Parkin, internally 
            tagged with mKO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Waters CS, Angenent SB, Altschuler SJ, Wu LF
  TITLE     A PINK1 input threshold arises from positive feedback in the 
            PINK1/Parkin mitophagy decision circuit.
  JOURNAL   Cell Rep. 2023 Oct 31;42(10):113260. doi: 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17.
  PUBMED    37851575
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-10-31"; volume: "42(10):113260. doi"; pages: " 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17"
COMMENT     Sequence Label: pLv-EF1a-Parkin(N273K)-A92mKO2
FEATURES             Location/Qualifiers
     source          1..9529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..232
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          233..1171
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1128..1148
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1486..2136
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             1486..2136
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     misc_feature    3298..3886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3351..3371)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3769..3780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4093..4726
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4854..4870)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4854..4870)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4867..4889)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4878..4894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4902..4908,4909..4926,4927..4932))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4947..4968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5085..5102)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5256..5844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5336..5355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6015..6806,6807..6875))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6638..6657
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6876..6980)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7028..7162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7065..7084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7119..7138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7330..7963
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8010..8135
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8632..8865
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9050..9094
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9357..9472
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cactcgaggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      241 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      301 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      361 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      421 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      481 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      541 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      601 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      661 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      721 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      781 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      841 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
      901 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
      961 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1021 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1081 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1141 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggacgcgtgc caccatgata
     1201 gtgtttgtca ggttcaactc cagccatggt ttcccagtgg aggtcgattc tgacaccagc
     1261 atcttccagc tcaaggaggt ggttgctaag cgacaggggg ttccggctga ccagttgcgt
     1321 gtgattttcg cagggaagga gctgaggaat gactggactg tgcagaattg tgacctggat
     1381 cagcagagca ttgttcacat tgtgcagaga ccgtggagaa aaggtcaaga aatgaatgca
     1441 actggaggcg acgaccccag aaacgcggcg ggtgctggtt ctggcgtgag cgtgatcaag
     1501 cccgagatga agatgaggta ctacatggac ggctccgtca atgggcatga gttcacaatc
     1561 gagggtgagg gcacaggcag accttacgag ggacatcagg agatgacact gcgcgtcaca
     1621 atggccgagg gcgggccaat gcctttcgcc ttcgacctgg tgtcccacgt gttctgttac
     1681 ggccacagag tttttaccaa gtacccagaa gagatcccag actatttcaa gcaggccttt
     1741 cctgagggcc tgtcctggga gaggtccctg gagttcgagg acggcggctc cgcctccgtg
     1801 agcgcccaca tcagcctgag gggcaacacc ttctaccaca agtccaagtt caccggcgtg
     1861 aacttccccg ccgacggccc catcatgcag aaccagagcg tggactggga gccctccacc
     1921 gagaagatca ccgccagcga cggcgtgctg aagggcgacg tgaccatgta cctgaagctg
     1981 gagggcggcg gcaaccacaa gtgccagatg aagaccacct acaaggccgc caaggagatc
     2041 ctggagatgc ccggcgacca ctacatcggc cacaggctgg tgaggaagac cgagggcaac
     2101 atcaccgagc aggtggagga cgccgtggcc cactcagcag gatcaggagg tggaggctgt
     2161 gagcgggagc cccagagctt gactcgggtg gacctcagca gctcagtcct cccaggagac
     2221 tctgtggggc tggctgtcat tctgcacact gacagcagga aggactcacc accagctgga
     2281 agtccagcag gtagatcaat ctacaacagc ttttatgtgt attgcaaagg cccctgtcaa
     2341 agagtgcagc cgggaaaact cagggtacag tgcagcacct gcaggcaggc aacgctcacc
     2401 ttgacccagg gtccatcttg ctgggatgat gttttaattc caaaccggat gagtggtgaa
     2461 tgccaatccc cacactgccc tgggactagt gcagaatttt tctttaaatg tggagcacac
     2521 cccacctctg acaaggaaac atcagtagct ttgcacctga tcgcaacaaa tagtcggaac
     2581 atcacttgca ttacgtgcac agacgtcagg agccccgtcc tggttttcca gtgcaactcc
     2641 cgccacgtga tttgcttaga ctgtttccac ttatactgtg tgacaagact caaagatcgg
     2701 cagtttgtcc acgaccctca gcttggctac tccctgcctt gtgtggctgg ctgtcccaac
     2761 tccttgatta aagagctcca tcacttcagg attctgggag aagagcagta caaccggtac
     2821 cagcagtatg gtgcagagga gtgtgtcctg cagatggggg gcgtgttatg cccccgccct
     2881 ggctgtggag cggggctgct gccggagcct gaccagagga aagtcacctg cgaagggggc
     2941 aatggcctgg gctgtgggtt tgccttctgc cgggaatgta aagaagcgta ccatgaaggg
     3001 gagtgcagtg ccgtatttga agcctcagga acaactactc aggcctacag agtcgatgaa
     3061 agagccgccg agcaggctcg ttgggaagca gcctccaaag aaaccatcaa gaaaaccacc
     3121 aagccctgtc cccgctgcca tgtaccagtg gaaaaaaatg gaggctgcat gcacatgaag
     3181 tgtccgcagc cccagtgcag gctcgagtgg tgctggaact gtggctgcga gtggaaccgc
     3241 gtctgcatgg gggaccactg gttcgacgtg taagatccaa tgcatcgcgt ctggaacaat
     3301 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3361 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3421 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3481 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3541 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3601 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3661 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     3721 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3781 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3841 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     3901 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     3961 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     4021 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4081 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     4141 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     4201 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     4261 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     4321 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     4381 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     4441 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     4501 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     4561 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4621 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4681 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     4741 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     4801 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     4861 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4921 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4981 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5041 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6121 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6721 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     7021 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7141 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     7201 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     7261 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     7321 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     7381 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     7441 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     7501 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     7561 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     7621 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     7681 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     7741 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7801 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7861 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7921 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     7981 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8041 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8101 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8161 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8221 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8281 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8341 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8401 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8461 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8521 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8581 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8641 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8701 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8761 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8821 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8881 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8941 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9001 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9061 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9121 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9181 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9241 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     9301 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta
     9361 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9421 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9481 attacaggga cagcagagat ccagtttatc gatgaggccc tttcgtctt
//
