LOCUS       sequence-420149-       10522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector to express cMyc-VSVG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pLV_CMV_cMyc-VSVG
FEATURES             Location/Qualifiers
     source          1..10522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(450..1241,1242..1310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1073..1092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1311..1415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1490..1509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1681..2060
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2062..2260
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2278..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2505..2630
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3123..3356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3541..3585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3883..4000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4035..4413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4414..4617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4662..4681
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4662..4680
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4783..4812
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6259..6291
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6307..6363
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7081..7137
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7138..7737
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7138..7157)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7634..7654
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7762..8350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7815..7835)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8233..8244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8353..8369)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8354..8370)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8875..9055
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9081..9098)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9087..9311
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9357..9785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9444..9463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9654..9675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9811..9827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9811..9827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9824..9846)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9835..9851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9859..9865,9866..9883,9884..9889))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9904..9925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10042..10059)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10213..10522,1..279))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10293..10312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
       61 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1441 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1501 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1561 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1621 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     1681 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     1741 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     1801 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     1861 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     1921 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     1981 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2041 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2101 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2161 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2221 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     2281 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2341 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2401 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2461 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2521 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2581 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     2641 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     2701 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     2761 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     2821 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2881 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2941 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     3001 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3061 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3121 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     3181 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     3241 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     3301 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     3361 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     3421 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     3481 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     3541 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     3601 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     3661 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     3721 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     3781 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     3841 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     3901 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     3961 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4021 cagagatcca gtttacattg attattgact agttattaat agtaatcaat tacggggtca
     4081 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     4141 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     4201 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     4261 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     4321 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     4381 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     4441 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4501 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4561 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     4621 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
     4681 gagacccaag ctggctagcc caccatgaag tgccttttgt acttagcctt tttattcatt
     4741 ggggtgaatt gcaagttcac catagttttt ccatccggag gagagcagaa gctgatttcc
     4801 gaggaggatc ttggcggaag cttgggacac aaccaaaaag gaaactggaa aaatgttcct
     4861 tctaattacc attattgccc gtcaagctca gatttaaatt ggcataatga cttaataggc
     4921 acagccttac aagtcaaaat gcccaagagt cacaaggcta ttcaagcaga cggttggatg
     4981 tgtcatgctt ccaaatgggt cactacttgt gatttccgct ggtatggacc gaagtatata
     5041 acacattcca tccgatcctt cactccatct gtagaacaat gcaaggaaag cattgaacaa
     5101 acgaaacaag gaacttggct gaatccaggc ttccctcctc aaagttgtgg atatgcaact
     5161 gtgacggatg ccgaagcagt gattgtccag gtgactcctc accatgtgct ggttgatgaa
     5221 tacacaggag aatgggttga ttcacagttc atcaacggaa aatgcagcaa ttacatatgc
     5281 cccactgtcc ataactctac aacctggcat tctgactata aggtcaaagg gctatgtgat
     5341 tctaacctca tttccatgga catcaccttc ttctcagagg acggagagct atcatccctg
     5401 ggaaaggagg gcacagggtt cagaagtaac tactttgctt atgaaactgg aggcaaggcc
     5461 tgcaaaatgc aatactgcaa gcattgggga gtcagactcc catcaggtgt ctggttcgag
     5521 atggctgata aggatctctt tgctgcagcc agattccctg aatgcccaga agggtcaagt
     5581 atctctgctc catctcagac ctcagtggat gtaagtctaa ttcaggacgt tgagaggatc
     5641 ttggattatt ccctctgcca agaaacctgg agcaaaatca gagcgggtct tccaatctct
     5701 ccagtggatc tcagctatct tgctcctaaa aacccaggaa ccggtcctgc tttcaccata
     5761 atcaatggta ccctaaaata ctttgagacc agatacatca gagtcgatat tgctgctcca
     5821 atcctctcaa gaatggtcgg aatgatcagt ggaactacca cagaaaggga actgtgggat
     5881 gactgggcac catatgaaga cgtggaaatt ggacccaatg gagttctgag gaccagttca
     5941 ggatataagt ttcctttata catgattgga catggtatgt tggactccga tcttcatctt
     6001 agctcaaagg ctcaggtgtt cgaacatcct cacattcaag acgctgcttc gcaacttcct
     6061 gatgatgaga gtttattttt tggtgatact gggctatcca aaaatccaat cgagcttgta
     6121 gaaggttggt tcagtagttg gaaaagctct attgcctctt ttttctttat catagggtta
     6181 atcattggac tattcttggt tctccgagtt ggtatccatc tttgcattaa attaaagcac
     6241 accaagaaaa gacagattta tacagacata gagatgaacc gacttggaaa ggaattcgga
     6301 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     6361 ccgatggtgt ccaagggcga ggaagataac atggccatca tcaaggagtt catgaggttt
     6421 aaggtccaca tggagggttc agtcaatggc cacgagttcg agattgaagg cgagggcgag
     6481 ggccgcccct acgaagggac acagacggcg aaattgaagg tgaccaaagg cgggccattg
     6541 cccttcgcat gggacatctt gtcccctcag tttatgtatg gcagcaaggc ctatgttaag
     6601 caccccgctg atatcccgga ctacttgaag ctgtcctttc cagaggggtt taaatgggag
     6661 cgcgttatga atttcgaaga cggaggagtg gttacggtga cgcaggactc atccctgcag
     6721 gacggagaat ttatatataa ggttaagttg agaggcacaa acttcccaag cgacggccct
     6781 gtgatgcaga agaaaacaat ggggtgggaa gcttccagcg agcgcatgta ccccgaagat
     6841 ggcgccctca agggcgagat aaagcaaagg ctgaaactta aggacggcgg tcattacgac
     6901 gcggaggtca agacaactta caaggctaaa aaacccgttc agttgcctgg ggcttacaat
     6961 gttaatatca aacttgacat cacaagccac aatgaagact atacgatcgt ggagcagtat
     7021 gaacgagcgg aaggcaggca ctcaacgggg gggatggacg agctttacaa gggatccggc
     7081 gcaacaaact tctctctgct caaacaagcc ggcgacgtcg aagagaatcc tgggccgatg
     7141 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     7201 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     7261 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     7321 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     7381 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     7441 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7501 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7561 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7621 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7681 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaagaa
     7741 ttcgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga aagattgact
     7801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8161 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8341 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     8401 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     8461 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     8521 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     8581 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     8641 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     8701 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     8761 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     8821 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     8881 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8941 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9001 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     9061 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     9121 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     9181 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     9241 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     9301 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     9361 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     9421 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     9481 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     9541 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     9601 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     9661 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9721 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9781 gaattaattc tgtagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9841 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9901 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9961 aaacctgtcg tgtcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10021 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10081 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10141 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10201 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10261 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10321 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10381 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10441 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10501 cttatccggt aactatcgtc tt
//
