LOCUS       sequence-420154-        9529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of S65A Parkin, internally 
            tagged with mKO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Waters CS, Angenent SB, Altschuler SJ, Wu LF
  TITLE     A PINK1 input threshold arises from positive feedback in the 
            PINK1/Parkin mitophagy decision circuit.
  JOURNAL   Cell Rep. 2023 Oct 31;42(10):113260. doi: 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17.
  PUBMED    37851575
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-10-31"; volume: "42(10):113260. doi"; pages: " 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17"
COMMENT     Sequence Label: pLv-EF1a-Parkin(S65A)-A92mKO2
FEATURES             Location/Qualifiers
     source          1..9529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..239
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          240..1178
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1135..1155
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1493..2143
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             1493..2143
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     misc_feature    3305..3893
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3358..3378)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3776..3787)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4100..4733
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4861..4877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4861..4877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4874..4896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4885..4901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4909..4915,4916..4933,4934..4939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4954..4975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5092..5109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5263..5851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5343..5362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6022..6813,6814..6882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6645..6664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6883..6987)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7035..7169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7072..7091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7126..7145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7337..7970
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8017..8142
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8639..8872
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9057..9101
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9364..9479
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tcgtcttcac tcgaggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
       61 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
      121 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      181 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
      241 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
      301 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
      361 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
      421 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
      481 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
      541 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
      601 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
      661 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
      721 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
      781 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
      841 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
      901 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
      961 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     1021 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     1081 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     1141 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga cgcgtgccac
     1201 catgatagtg tttgtcaggt tcaactccag ccatggtttc ccagtggagg tcgattctga
     1261 caccagcatc ttccagctca aggaggtggt tgctaagcga cagggggttc cggctgacca
     1321 gttgcgtgtg attttcgcag ggaaggagct gaggaatgac tggactgtgc agaattgtga
     1381 cctggatcag caggccattg ttcacattgt gcagagaccg tggagaaaag gtcaagaaat
     1441 gaatgcaact ggaggcgacg accccagaaa cgcggcgggt gctggttctg gcgtgagcgt
     1501 gatcaagccc gagatgaaga tgaggtacta catggacggc tccgtcaatg ggcatgagtt
     1561 cacaatcgag ggtgagggca caggcagacc ttacgaggga catcaggaga tgacactgcg
     1621 cgtcacaatg gccgagggcg ggccaatgcc tttcgccttc gacctggtgt cccacgtgtt
     1681 ctgttacggc cacagagttt ttaccaagta cccagaagag atcccagact atttcaagca
     1741 ggcctttcct gagggcctgt cctgggagag gtccctggag ttcgaggacg gcggctccgc
     1801 ctccgtgagc gcccacatca gcctgagggg caacaccttc taccacaagt ccaagttcac
     1861 cggcgtgaac ttccccgccg acggccccat catgcagaac cagagcgtgg actgggagcc
     1921 ctccaccgag aagatcaccg ccagcgacgg cgtgctgaag ggcgacgtga ccatgtacct
     1981 gaagctggag ggcggcggca accacaagtg ccagatgaag accacctaca aggccgccaa
     2041 ggagatcctg gagatgcccg gcgaccacta catcggccac aggctggtga ggaagaccga
     2101 gggcaacatc accgagcagg tggaggacgc cgtggcccac tcagcaggat caggaggtgg
     2161 aggctgtgag cgggagcccc agagcttgac tcgggtggac ctcagcagct cagtcctccc
     2221 aggagactct gtggggctgg ctgtcattct gcacactgac agcaggaagg actcaccacc
     2281 agctggaagt ccagcaggta gatcaatcta caacagcttt tatgtgtatt gcaaaggccc
     2341 ctgtcaaaga gtgcagccgg gaaaactcag ggtacagtgc agcacctgca ggcaggcaac
     2401 gctcaccttg acccagggtc catcttgctg ggatgatgtt ttaattccaa accggatgag
     2461 tggtgaatgc caatccccac actgccctgg gactagtgca gaatttttct ttaaatgtgg
     2521 agcacacccc acctctgaca aggaaacatc agtagctttg cacctgatcg caacaaatag
     2581 tcggaacatc acttgcatta cgtgcacaga cgtcaggagc cccgtcctgg ttttccagtg
     2641 caactcccgc cacgtgattt gcttagactg tttccactta tactgtgtga caagactcaa
     2701 tgatcggcag tttgtccacg accctcagct tggctactcc ctgccttgtg tggctggctg
     2761 tcccaactcc ttgattaaag agctccatca cttcaggatt ctgggagaag agcagtacaa
     2821 ccggtaccag cagtatggtg cagaggagtg tgtcctgcag atggggggcg tgttatgccc
     2881 ccgccctggc tgtggagcgg ggctgctgcc ggagcctgac cagaggaaag tcacctgcga
     2941 agggggcaat ggcctgggct gtgggtttgc cttctgccgg gaatgtaaag aagcgtacca
     3001 tgaaggggag tgcagtgccg tatttgaagc ctcaggaaca actactcagg cctacagagt
     3061 cgatgaaaga gccgccgagc aggctcgttg ggaagcagcc tccaaagaaa ccatcaagaa
     3121 aaccaccaag ccctgtcccc gctgccatgt accagtggaa aaaaatggag gctgcatgca
     3181 catgaagtgt ccgcagcccc agtgcaggct cgagtggtgc tggaactgtg gctgcgagtg
     3241 gaaccgcgtc tgcatggggg accactggtt cgacgtgtaa gatccaatgc atcgcgtctg
     3301 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3361 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3421 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3481 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3541 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3601 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3661 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     3721 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3781 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3841 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     3901 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     3961 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     4021 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4081 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     4141 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     4201 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     4261 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     4321 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     4381 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     4441 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     4501 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     4561 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4621 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4681 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     4741 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4801 gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg
     4861 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4921 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4981 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5041 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5101 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5701 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6001 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6061 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6121 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6181 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6241 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6301 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6361 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6421 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6481 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6541 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6601 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6661 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6721 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6781 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6841 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6901 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     7021 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7081 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7141 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     7201 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     7261 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     7321 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     7381 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     7441 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     7501 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     7561 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     7621 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     7681 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     7741 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     7801 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7861 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7921 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7981 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     8041 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     8101 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     8161 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     8221 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     8281 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     8341 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8401 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8461 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     8521 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8581 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8641 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8701 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8761 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8821 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8881 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8941 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     9001 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9061 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9121 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9181 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9241 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     9301 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     9361 gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     9421 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     9481 cgggtttatt acagggacag cagagatcca gtttatcgat gaggccctt
//
