LOCUS       sequence-420156-        9529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of R104A Parkin, internally 
            tagged with mKO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Waters CS, Angenent SB, Altschuler SJ, Wu LF
  TITLE     A PINK1 input threshold arises from positive feedback in the 
            PINK1/Parkin mitophagy decision circuit.
  JOURNAL   Cell Rep. 2023 Oct 31;42(10):113260. doi: 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17.
  PUBMED    37851575
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-10-31"; volume: "42(10):113260. doi"; pages: " 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17"
COMMENT     Sequence Label: pLv-EF1a-Parkin(R104A)-A92mKO2
FEATURES             Location/Qualifiers
     source          1..9529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..238
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          239..1177
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1134..1154
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1492..2142
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             1492..2142
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     misc_feature    3304..3892
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3357..3377)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3775..3786)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4099..4732
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4860..4876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4860..4876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4873..4895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4884..4900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4908..4914,4915..4932,4933..4938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4953..4974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5091..5108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5262..5850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5342..5361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6021..6812,6813..6881))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6644..6663
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6882..6986)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7034..7168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7071..7090)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7125..7144
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7336..7969
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8016..8141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8638..8871
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9056..9100
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9363..9478
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgtcttcact cgaggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
       61 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
      121 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
      181 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
      241 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
      301 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
      361 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
      421 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
      481 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
      541 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
      601 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
      661 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
      721 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
      781 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
      841 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
      901 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
      961 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     1021 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     1081 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     1141 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggac gcgtgccacc
     1201 atgatagtgt ttgtcaggtt caactccagc catggtttcc cagtggaggt cgattctgac
     1261 accagcatct tccagctcaa ggaggtggtt gctaagcgac agggggttcc ggctgaccag
     1321 ttgcgtgtga ttttcgcagg gaaggagctg aggaatgact ggactgtgca gaattgtgac
     1381 ctggatcagc agagcattgt tcacattgtg cagagaccgt ggagaaaagg tcaagaaatg
     1441 aatgcaactg gaggcgacga ccccagaaac gcggcgggtg ctggttctgg cgtgagcgtg
     1501 atcaagcccg agatgaagat gaggtactac atggacggct ccgtcaatgg gcatgagttc
     1561 acaatcgagg gtgagggcac aggcagacct tacgagggac atcaggagat gacactgcgc
     1621 gtcacaatgg ccgagggcgg gccaatgcct ttcgccttcg acctggtgtc ccacgtgttc
     1681 tgttacggcc acagagtttt taccaagtac ccagaagaga tcccagacta tttcaagcag
     1741 gcctttcctg agggcctgtc ctgggagagg tccctggagt tcgaggacgg cggctccgcc
     1801 tccgtgagcg cccacatcag cctgaggggc aacaccttct accacaagtc caagttcacc
     1861 ggcgtgaact tccccgccga cggccccatc atgcagaacc agagcgtgga ctgggagccc
     1921 tccaccgaga agatcaccgc cagcgacggc gtgctgaagg gcgacgtgac catgtacctg
     1981 aagctggagg gcggcggcaa ccacaagtgc cagatgaaga ccacctacaa ggccgccaag
     2041 gagatcctgg agatgcccgg cgaccactac atcggccaca ggctggtgag gaagaccgag
     2101 ggcaacatca ccgagcaggt ggaggacgcc gtggcccact cagcaggatc aggaggtgga
     2161 ggctgtgagc gggagcccca gagcttgact gcggtggacc tcagcagctc agtcctccca
     2221 ggagactctg tggggctggc tgtcattctg cacactgaca gcaggaagga ctcaccacca
     2281 gctggaagtc cagcaggtag atcaatctac aacagctttt atgtgtattg caaaggcccc
     2341 tgtcaaagag tgcagccggg aaaactcagg gtacagtgca gcacctgcag gcaggcaacg
     2401 ctcaccttga cccagggtcc atcttgctgg gatgatgttt taattccaaa ccggatgagt
     2461 ggtgaatgcc aatccccaca ctgccctggg actagtgcag aatttttctt taaatgtgga
     2521 gcacacccca cctctgacaa ggaaacatca gtagctttgc acctgatcgc aacaaatagt
     2581 cggaacatca cttgcattac gtgcacagac gtcaggagcc ccgtcctggt tttccagtgc
     2641 aactcccgcc acgtgatttg cttagactgt ttccacttat actgtgtgac aagactcaat
     2701 gatcggcagt ttgtccacga ccctcagctt ggctactccc tgccttgtgt ggctggctgt
     2761 cccaactcct tgattaaaga gctccatcac ttcaggattc tgggagaaga gcagtacaac
     2821 cggtaccagc agtatggtgc agaggagtgt gtcctgcaga tggggggcgt gttatgcccc
     2881 cgccctggct gtggagcggg gctgctgccg gagcctgacc agaggaaagt cacctgcgaa
     2941 gggggcaatg gcctgggctg tgggtttgcc ttctgccggg aatgtaaaga agcgtaccat
     3001 gaaggggagt gcagtgccgt atttgaagcc tcaggaacaa ctactcaggc ctacagagtc
     3061 gatgaaagag ccgccgagca ggctcgttgg gaagcagcct ccaaagaaac catcaagaaa
     3121 accaccaagc cctgtccccg ctgccatgta ccagtggaaa aaaatggagg ctgcatgcac
     3181 atgaagtgtc cgcagcccca gtgcaggctc gagtggtgct ggaactgtgg ctgcgagtgg
     3241 aaccgcgtct gcatggggga ccactggttc gacgtgtaag atccaatgca tcgcgtctgg
     3301 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3361 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3421 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3481 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3541 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3601 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3661 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     3721 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3781 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3841 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     3901 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     3961 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     4021 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4081 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     4141 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     4201 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     4261 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     4321 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     4381 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     4441 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     4501 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     4561 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4621 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4681 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     4741 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     4801 aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg
     4861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5701 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     7021 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7081 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7141 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     7201 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     7261 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     7321 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     7381 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     7441 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     7501 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     7561 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     7621 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     7681 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     7741 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     7801 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7861 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7921 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     7981 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8041 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8101 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8161 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8221 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8281 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8341 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8401 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8461 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     8521 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8581 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8641 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8701 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8761 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8821 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8881 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8941 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9001 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9061 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9121 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9181 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9241 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     9301 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     9361 cctttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9421 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9481 gggtttatta cagggacagc agagatccag tttatcgatg aggcccttt
//
