LOCUS       sequence-420157-        9529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of K161N Parkin, internally 
            tagged with mKO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Waters CS, Angenent SB, Altschuler SJ, Wu LF
  TITLE     A PINK1 input threshold arises from positive feedback in the 
            PINK1/Parkin mitophagy decision circuit.
  JOURNAL   Cell Rep. 2023 Oct 31;42(10):113260. doi: 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17.
  PUBMED    37851575
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-10-31"; volume: "42(10):113260. doi"; pages: " 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17"
COMMENT     Sequence Label: pLv-EF1a-Parkin(K161N)-A92mKO2
FEATURES             Location/Qualifiers
     source          1..9529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..234
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          235..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1488..2138
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             1488..2138
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     misc_feature    3300..3888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3353..3373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3771..3782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4095..4728
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4856..4872)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4856..4872)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4869..4891)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4880..4896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4904..4910,4911..4928,4929..4934))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4949..4970
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5087..5104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5258..5846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5338..5357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6017..6808,6809..6877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6640..6659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6878..6982)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7030..7164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7067..7086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7121..7140
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7332..7965
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8012..8137
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8634..8867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9052..9096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9359..9474
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 ttcactcgag gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
       61 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      121 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      181 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      241 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      301 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggacgcgt gccaccatga
     1201 tagtgtttgt caggttcaac tccagccatg gtttcccagt ggaggtcgat tctgacacca
     1261 gcatcttcca gctcaaggag gtggttgcta agcgacaggg ggttccggct gaccagttgc
     1321 gtgtgatttt cgcagggaag gagctgagga atgactggac tgtgcagaat tgtgacctgg
     1381 atcagcagag cattgttcac attgtgcaga gaccgtggag aaaaggtcaa gaaatgaatg
     1441 caactggagg cgacgacccc agaaacgcgg cgggtgctgg ttctggcgtg agcgtgatca
     1501 agcccgagat gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa
     1561 tcgagggtga gggcacaggc agaccttacg agggacatca ggagatgaca ctgcgcgtca
     1621 caatggccga gggcgggcca atgcctttcg ccttcgacct ggtgtcccac gtgttctgtt
     1681 acggccacag agtttttacc aagtacccag aagagatccc agactatttc aagcaggcct
     1741 ttcctgaggg cctgtcctgg gagaggtccc tggagttcga ggacggcggc tccgcctccg
     1801 tgagcgccca catcagcctg aggggcaaca ccttctacca caagtccaag ttcaccggcg
     1861 tgaacttccc cgccgacggc cccatcatgc agaaccagag cgtggactgg gagccctcca
     1921 ccgagaagat caccgccagc gacggcgtgc tgaagggcga cgtgaccatg tacctgaagc
     1981 tggagggcgg cggcaaccac aagtgccaga tgaagaccac ctacaaggcc gccaaggaga
     2041 tcctggagat gcccggcgac cactacatcg gccacaggct ggtgaggaag accgagggca
     2101 acatcaccga gcaggtggag gacgccgtgg cccactcagc aggatcagga ggtggaggct
     2161 gtgagcggga gccccagagc ttgactcggg tggacctcag cagctcagtc ctcccaggag
     2221 actctgtggg gctggctgtc attctgcaca ctgacagcag gaaggactca ccaccagctg
     2281 gaagtccagc aggtagatca atctacaaca gcttttatgt gtattgcaaa ggcccctgtc
     2341 aaagagtgca gccgggaaat ctcagggtac agtgcagcac ctgcaggcag gcaacgctca
     2401 ccttgaccca gggtccatct tgctgggatg atgttttaat tccaaaccgg atgagtggtg
     2461 aatgccaatc cccacactgc cctgggacta gtgcagaatt tttctttaaa tgtggagcac
     2521 accccacctc tgacaaggaa acatcagtag ctttgcacct gatcgcaaca aatagtcgga
     2581 acatcacttg cattacgtgc acagacgtca ggagccccgt cctggttttc cagtgcaact
     2641 cccgccacgt gatttgctta gactgtttcc acttatactg tgtgacaaga ctcaatgatc
     2701 ggcagtttgt ccacgaccct cagcttggct actccctgcc ttgtgtggct ggctgtccca
     2761 actccttgat taaagagctc catcacttca ggattctggg agaagagcag tacaaccggt
     2821 accagcagta tggtgcagag gagtgtgtcc tgcagatggg gggcgtgtta tgcccccgcc
     2881 ctggctgtgg agcggggctg ctgccggagc ctgaccagag gaaagtcacc tgcgaagggg
     2941 gcaatggcct gggctgtggg tttgccttct gccgggaatg taaagaagcg taccatgaag
     3001 gggagtgcag tgccgtattt gaagcctcag gaacaactac tcaggcctac agagtcgatg
     3061 aaagagccgc cgagcaggct cgttgggaag cagcctccaa agaaaccatc aagaaaacca
     3121 ccaagccctg tccccgctgc catgtaccag tggaaaaaaa tggaggctgc atgcacatga
     3181 agtgtccgca gccccagtgc aggctcgagt ggtgctggaa ctgtggctgc gagtggaacc
     3241 gcgtctgcat gggggaccac tggttcgacg tgtaagatcc aatgcatcgc gtctggaaca
     3301 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3361 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3421 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3481 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3541 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3601 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3661 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     3721 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3781 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3841 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     3901 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     3961 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4021 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4081 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     4141 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     4201 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     4261 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     4321 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     4381 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     4441 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     4501 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     4561 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4621 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4681 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     4741 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     4801 cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     4861 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4921 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     4981 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5041 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5101 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5161 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5221 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5281 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5341 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5401 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5461 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5521 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5581 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5641 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5701 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5761 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5821 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5881 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5941 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6001 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6061 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6121 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6181 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6241 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6301 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6361 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6421 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6481 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6541 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6601 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6661 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6721 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6781 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6841 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6901 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6961 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7021 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7081 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7141 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     7201 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     7261 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     7321 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     7381 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     7441 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     7501 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     7561 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     7621 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     7681 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     7741 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     7801 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7861 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7921 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     7981 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8041 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8101 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8161 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8221 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8281 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8341 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     8401 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8461 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8521 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     8581 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     8641 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     8701 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     8761 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8821 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     8881 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     8941 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9001 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9061 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9121 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9181 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9241 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     9301 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgcctt
     9361 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     9421 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     9481 ttattacagg gacagcagag atccagttta tcgatgaggc cctttcgtc
//
