LOCUS       sequence-420158-        9529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of Parkin, internally tagged
            with mKO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Waters CS, Angenent SB, Altschuler SJ, Wu LF
  TITLE     A PINK1 input threshold arises from positive feedback in the 
            PINK1/Parkin mitophagy decision circuit.
  JOURNAL   Cell Rep. 2023 Oct 31;42(10):113260. doi: 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17.
  PUBMED    37851575
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-10-31"; volume: "42(10):113260. doi"; pages: " 
            10.1016/j.celrep.2023.113260. Epub 2023 Oct 17"
COMMENT     Sequence Label: pLv-EF1a-Parkin-A92mKO2
FEATURES             Location/Qualifiers
     source          1..9529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..240
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          241..1179
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1136..1156
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1494..2144
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             1494..2144
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     misc_feature    3306..3894
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3359..3379)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3777..3788)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4101..4734
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4862..4878)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4862..4878)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4875..4897)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4886..4902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4910..4916,4917..4934,4935..4940))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4955..4976
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5093..5110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5264..5852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5344..5363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6023..6814,6815..6883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6646..6665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6884..6988)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7036..7170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7073..7092)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7127..7146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7338..7971
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8018..8143
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8640..8873
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9058..9102
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9365..9480
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 ttcgtcttca ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
       61 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
      121 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      181 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      241 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
      301 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      361 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      421 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      481 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      541 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
      601 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
      661 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
      721 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
      781 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
      841 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
      901 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
      961 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1021 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1081 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1141 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg acgcgtgcca
     1201 ccatgatagt gtttgtcagg ttcaactcca gccatggttt cccagtggag gtcgattctg
     1261 acaccagcat cttccagctc aaggaggtgg ttgctaagcg acagggggtt ccggctgacc
     1321 agttgcgtgt gattttcgca gggaaggagc tgaggaatga ctggactgtg cagaattgtg
     1381 acctggatca gcagagcatt gttcacattg tgcagagacc gtggagaaaa ggtcaagaaa
     1441 tgaatgcaac tggaggcgac gaccccagaa acgcggcggg tgctggttct ggcgtgagcg
     1501 tgatcaagcc cgagatgaag atgaggtact acatggacgg ctccgtcaat gggcatgagt
     1561 tcacaatcga gggtgagggc acaggcagac cttacgaggg acatcaggag atgacactgc
     1621 gcgtcacaat ggccgagggc gggccaatgc ctttcgcctt cgacctggtg tcccacgtgt
     1681 tctgttacgg ccacagagtt tttaccaagt acccagaaga gatcccagac tatttcaagc
     1741 aggcctttcc tgagggcctg tcctgggaga ggtccctgga gttcgaggac ggcggctccg
     1801 cctccgtgag cgcccacatc agcctgaggg gcaacacctt ctaccacaag tccaagttca
     1861 ccggcgtgaa cttccccgcc gacggcccca tcatgcagaa ccagagcgtg gactgggagc
     1921 cctccaccga gaagatcacc gccagcgacg gcgtgctgaa gggcgacgtg accatgtacc
     1981 tgaagctgga gggcggcggc aaccacaagt gccagatgaa gaccacctac aaggccgcca
     2041 aggagatcct ggagatgccc ggcgaccact acatcggcca caggctggtg aggaagaccg
     2101 agggcaacat caccgagcag gtggaggacg ccgtggccca ctcagcagga tcaggaggtg
     2161 gaggctgtga gcgggagccc cagagcttga ctcgggtgga cctcagcagc tcagtcctcc
     2221 caggagactc tgtggggctg gctgtcattc tgcacactga cagcaggaag gactcaccac
     2281 cagctggaag tccagcaggt agatcaatct acaacagctt ttatgtgtat tgcaaaggcc
     2341 cctgtcaaag agtgcagccg ggaaaactca gggtacagtg cagcacctgc aggcaggcaa
     2401 cgctcacctt gacccagggt ccatcttgct gggatgatgt tttaattcca aaccggatga
     2461 gtggtgaatg ccaatcccca cactgccctg ggactagtgc agaatttttc tttaaatgtg
     2521 gagcacaccc cacctctgac aaggaaacat cagtagcttt gcacctgatc gcaacaaata
     2581 gtcggaacat cacttgcatt acgtgcacag acgtcaggag ccccgtcctg gttttccagt
     2641 gcaactcccg ccacgtgatt tgcttagact gtttccactt atactgtgtg acaagactca
     2701 atgatcggca gtttgtccac gaccctcagc ttggctactc cctgccttgt gtggctggct
     2761 gtcccaactc cttgattaaa gagctccatc acttcaggat tctgggagaa gagcagtaca
     2821 accggtacca gcagtatggt gcagaggagt gtgtcctgca gatggggggc gtgttatgcc
     2881 cccgccctgg ctgtggagcg gggctgctgc cggagcctga ccagaggaaa gtcacctgcg
     2941 aagggggcaa tggcctgggc tgtgggtttg ccttctgccg ggaatgtaaa gaagcgtacc
     3001 atgaagggga gtgcagtgcc gtatttgaag cctcaggaac aactactcag gcctacagag
     3061 tcgatgaaag agccgccgag caggctcgtt gggaagcagc ctccaaagaa accatcaaga
     3121 aaaccaccaa gccctgtccc cgctgccatg taccagtgga aaaaaatgga ggctgcatgc
     3181 acatgaagtg tccgcagccc cagtgcaggc tcgagtggtg ctggaactgt ggctgcgagt
     3241 ggaaccgcgt ctgcatgggg gaccactggt tcgacgtgta agatccaatg catcgcgtct
     3301 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3361 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3421 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3481 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3541 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3601 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3661 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     3721 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3781 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3841 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     3901 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     3961 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     4021 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4081 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     4141 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     4201 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     4261 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     4321 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     4381 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     4441 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     4501 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     4561 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4621 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4681 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     4741 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4801 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
     4861 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4921 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4981 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5041 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5101 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5161 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5221 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5281 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5341 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5401 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5461 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5521 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5581 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5641 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5701 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5761 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5821 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5881 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5941 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6001 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6061 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6121 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6181 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6241 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6301 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6361 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6421 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6481 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6541 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6601 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6661 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6721 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6781 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6841 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6901 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6961 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     7021 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7141 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     7201 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     7261 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     7321 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     7381 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     7441 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     7501 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     7561 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     7621 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     7681 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     7741 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     7801 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7861 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7921 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     7981 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     8041 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     8101 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     8161 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     8221 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     8281 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     8341 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     8401 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     8461 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     8521 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     8581 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     8641 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     8701 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     8761 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     8821 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     8881 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     8941 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     9001 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     9061 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     9121 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     9181 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     9241 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     9301 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     9361 cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     9421 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     9481 tcgggtttat tacagggaca gcagagatcc agtttatcga tgaggccct
//
