LOCUS       sequence-420219-        2773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part of Bactoneuron DF2, expresses mvenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Srivastava R, Bagh S
  TITLE     A Logically Reversible Double Feynman Gate with Molecular Engineered
            Bacteria Arranged in an Artificial Neural Network-Type Architecture.
  JOURNAL   ACS Synth Biol. 2023 Jan 20;12(1):51-60. doi: 
            10.1021/acssynbio.2c00520. Epub 2022 Nov 16.
  PUBMED    36384003
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00520"; journalName: "ACS Synth Biol"; date: 
            "2023-01-20- 20"; volume: "12"; issue: "1"; pages: "51-60"
COMMENT     Sequence Label: pHC_O2
FEATURES             Location/Qualifiers
     source          1..2773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(226..320)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(341..1000)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     915..934
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1001..1103)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1169..1187
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             1336..1347
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1354..1356,1357..1359,1360..2073)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     ERVTAAGITLGMDELYK"
     primer_bind     complement(1399..1420)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1660..1679)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     terminator      2085..2171
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2206..2223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2340..2773,1..112))
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2420..2439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc
       61 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
      121 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
      181 acctgaagtc agccccatac gatataagtt gttactagtg cttggattct caccaataaa
      241 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      301 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
      361 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
      421 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
      481 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
      541 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
      601 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
      661 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
      721 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
      781 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
      841 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
      901 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      961 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1021 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1081 tggaacctct tacgtgccga tcaacgtctc attttcgcca gatatcgacg tctaagaaac
     1141 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     1201 cctcgagacc tgtaggatcg tacaggttta cgtacctctg gcggtgatat atagtcgaat
     1261 aaatacctct ggcggtgata gatgattacc tctggcggtg atagatgatt acctctggcg
     1321 gtgatagaat tcattaaaga ggagaaaggt accatggtga gcaagggcga ggagctgttc
     1381 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1441 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     1501 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg
     1561 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1621 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1681 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1741 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1801 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     1861 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     1921 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     1981 aaagacccca acgagaagcg cgatcacatg gtcctgctgg aacgcgtgac cgccgccggg
     2041 atcactctcg gcatggacga gctgtacaag taatctagag gcatcaaata aaacgaaagg
     2101 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     2161 gtaggacaaa tccgccgccc tagacctagg gatatattcc gcttcctcgc tcactgactc
     2221 gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt
     2281 cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt
     2341 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     2401 cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc
     2461 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     2521 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     2581 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2641 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
     2701 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
     2761 ctcctccaag cca
//
