LOCUS       sequence-420222-       11570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MS2 hairpin-containing expression vector and sgRNA entry plasmid for
            the directed export of barcode transcripts in Gag-MCP virus-like 
            particles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 11570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: CROPseq-Puro-24xMS2
FEATURES             Location/Qualifiers
     source          1..11570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2773..2890
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3029..4207
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3260..4198
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4155..4175
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4220..4819
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4220..4239)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4716..4736
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_RNA        4876..4894
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4915..4933
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4985..5003
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5024..5042
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5094..5112
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5133..5151
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5203..5221
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5242..5260
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5312..5330
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5351..5369
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5421..5439
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5460..5478
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5530..5548
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5569..5587
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5639..5657
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5678..5696
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5748..5766
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5787..5805
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5857..5875
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5896..5914
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5966..5984
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6005..6023
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6075..6093
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6114..6132
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_feature    6181..6769
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6234..6254)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6652..6663)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6925..7173
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6925..6945
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7096..7115
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             9193..9373
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9451..9572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9488..9507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9542..9561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9612..9747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9767..9786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9768..9786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9796..9813)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9796..9812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9805..9827)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9954..10409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10041..10060)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10251..10272
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10435..10539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10540..10608,10609..11400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10758..10777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgac acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga atcttgagac aaatggcagt
     2761 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2821 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2881 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     2941 ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     3001 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     3061 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     3121 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3181 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3301 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     3361 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     3421 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     3481 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     3541 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     3601 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     3661 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     3721 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     3781 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     3841 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     3901 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     3961 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     4021 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     4081 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     4141 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     4201 gtcgtgacgt acggccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4261 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4321 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4381 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     4441 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4501 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4561 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca
     4621 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4681 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4741 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4801 ccgcaagccc ggtgcctgat ggacgagctg tacaagtaaa gcggccggat cctaaggtac
     4861 ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcta gaaaacatga
     4921 ggatcaccca tgtctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta
     4981 gaaaacatga ggatcaccca tgtctgcagg tcgactctag aaaacatgag gatcacccat
     5041 gtctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaacatgag
     5101 gatcacccat gtctgcaggt cgactccaga aaacatgagg atcacccatg tctgcagtat
     5161 tcccgggttc attagatcct aaggtaccta attgcctaga aaacatgagg atcacccatg
     5221 tctgcaggtc gactctagaa aacatgagga tcacccatgt ctgcagtatt cccgggttca
     5281 ttagatccta aggtacctaa ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg
     5341 actctagaaa acatgaggat cacccatgtc tgcagtattc ccgggttcat tagatcctaa
     5401 ggtacctaat tgcctagaaa acatgaggat cacccatgtc tgcaggtcga ctccagaaaa
     5461 catgaggatc acccatgtct gcagtattcc cgggttcatt agatcctaag gtacctaatt
     5521 gcctagaaaa catgaggatc acccatgtct gcaggtcgac tctagaaaac atgaggatca
     5581 cccatgtctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaaac
     5641 atgaggatca cccatgtctg caggtcgact ctagaaaaca tgaggatcac ccatgtctgc
     5701 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaaaca tgaggatcac
     5761 ccatgtctgc aggtcgactc tagaaaacat gaggatcacc catgtctgca gtattcccgg
     5821 gttcattaga tcctaaggta cctaattgcc tagaaaacat gaggatcacc catgtctgca
     5881 ggtcgactct agaaaacatg aggatcaccc atgtctgcag tattcccggg ttcattagat
     5941 cctaaggtac ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcta
     6001 gaaaacatga ggatcaccca tgtctgcagt attcccgggt tcattagatc ctaaggtacc
     6061 taattgccta gaaaacatga ggatcaccca tgtctgcagg tcgactccag aaaacatgag
     6121 gatcacccat gtctgcagta ttcccgggtt cattagatct gcgcgcgatc gatagatcct
     6181 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6241 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6301 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6361 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6421 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6481 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6541 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6601 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6661 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6721 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     6781 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6841 tggaagggct aattcactcc caacgaagac aagatttcac cattatcgtt tcagacccac
     6901 ctcccaaccc cgaggggacc cagagagggc ctatttccca tgattccttc atatttgcat
     6961 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
     7021 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     7081 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     7141 gctttatata tcttgtggaa aggacgaaac accggagacg gttgtaaatg agcacacaaa
     7201 atacacatgc taaaatatta tattctatga cctttataaa atcaaccaaa atcttctttt
     7261 taataacttt agtatcaata attagaattt ttatgttcct ttttgcaaac ttttaataaa
     7321 aatgagcaaa ataaaaaaac gctagtttta gtaactcgcg ttgttttctt cacctttaat
     7381 aatagctact ccaccacttg ttcctaagcg gtcagctcct gcttcaatca ttttttgagc
     7441 atcttcaaat gttctaactc caccagctgc tttaactaaa gcattgtctt taacaactga
     7501 cttcattagt ttaacatctt caaatgttgc acctgatttt gaaaatcctg ttgatgtttt
     7561 aacaaattct aatccagctt caacagctat ttcacaagct ttcatgattt cttcttttgt
     7621 taataaacaa ttttccataa tacatttaac aacatgtgat ccagctgctt tttttacagc
     7681 tttcatgtct tctaaaacta attcataatt tttgtctttt aatgcaccaa tatttaatac
     7741 catatcaatt tctgttgcac catctttaat tgcttcagaa acttcgaatg cttttgtagc
     7801 tgttgtgcat gcacctagag gaaaacctac aacatttgtt attcctacat ttgtgccttt
     7861 taataattct ttacaatagc ttgttcaata tgaattaaca caaactgttg caaaatcaaa
     7921 ttcaattgct tcatcacata attgtttaat ttcagctttc gtagcatctt gttttaataa
     7981 tgtgtgatct atatatttgt ttagtttcat tttttctcct atatattcat ttttaatttt
     8041 aattctttaa taatttcgtc tactttaact ttagcgtttt gaacagattc accaacacct
     8101 ataaaataaa tttttagttt aggttcagtt ccacttgggc gaacagcaaa tcatgactta
     8161 tcttctaaat aaaattttag taagtcttgt cctggcatat tatacattcc atcgatgtag
     8221 tcttcaacat taacaacttt aagtccagca atttgagtta agggtgttgc tctcaatgat
     8281 ttcattaatg gttcaatttt taatttcttt tcttctggtt taaaattcaa gtttaaagtg
     8341 aaagtgtaat atgcacccat ttctttaaat aaatcttcta aatagtctac taatgtttta
     8401 ttttgttttt tataaaatca agcagcctct gctattaata tagaagcttg tattccatct
     8461 ttatctctag ctgagtcatc aattacatat ccataacttt cttcataagc aaaaacaaaa
     8521 tttaatccgt tatcttcttc tttagcaatt tctctaccca ttcatttaaa tccagttaaa
     8581 gtttttacaa tattaactcc atatttttca tgagcgattc tatcacccaa atcacttgtt
     8641 acaaaacttg aatatagagc cggatttttt ggaatgctat ttaagcgttt tagatttgat
     8701 aattttcaat caattaaaat tggtcctgtt tgatttccat ctaatcttac aaaatgacca
     8761 tcatgtttta ttgccattcc aaatctgtca gcatctgggt cattcataat aataatatct
     8821 gcatcatgtt taataccata ttcaagcggt atttttcatg caggatcaaa ttctggattt
     8881 ggatttacaa catttttaaa tgtttcatct tcaaatgcat gctcttcaac ctcaataacg
     8941 ttatatcctg attcacgtaa tatttttggg gtaaatttag ttcctgttcc attaactgcg
     9001 ctaaaaataa tttttaaatc ttttttagct tcttgctctt ttttgtacgt ctctgtttca
     9061 gagctatgct ggaaacagca tagcaagttg aaataaggct agtccgttat caacttgaaa
     9121 aagtggcacc gagtcggtgc ttttttaagc ttggcgtaac tagatcttga gacactgctt
     9181 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9241 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9301 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9361 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     9421 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     9481 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9541 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     9601 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9661 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9721 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     9781 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     9841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     9961 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
    10021 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
    10081 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
    10141 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
    10201 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
    10261 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
    10321 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
    10381 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
    10441 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    10501 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    10561 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    10621 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    10681 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10741 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
    10801 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10861 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10921 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10981 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    11041 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    11101 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    11161 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    11221 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    11281 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    11341 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    11401 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    11461 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    11521 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
