LOCUS       sequence-420225-        6413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Construct for stable expression of constitutively active mouse Ptk6 
            (Sik) in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Derry JJ, Richard S, Valderrama Carvajal H, Ye X, Vasioukhin V, 
            Cochrane AW, Chen T, Tyner AL
  TITLE     Sik (BRK) phosphorylates Sam68 in the nucleus and negatively 
            regulates its RNA binding ability.
  JOURNAL   Mol Cell Biol. 2000 Aug;20(16):6114-26. doi: 
            10.1128/MCB.20.16.6114-6126.2000.
  PUBMED    10913193
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.20.16.6114-6126"; journalName: "Mol Cell Biol"; date: 
            "2000-08"; volume: "20"; issue: "16"; pages: "6114-26"
COMMENT     Sequence Label: pBabe-Sik-YF
FEATURES             Location/Qualifiers
     source          1..6413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(154..945,946..1014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     777..796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1015..1119)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1150..1619
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1684..1883
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2084..2500
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2430..2452
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2468..2484
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3957..3977)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4001..4211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4023..4219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4070..4205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4229..4828
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4229..4248)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4725..4745
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5049..5518
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5648..5665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5819..6407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5899..5918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
       61 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc gcaaatcaat
      121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
      301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1141 acctgcagcc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     1201 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     1261 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     1321 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     1381 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     1441 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     1501 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     1561 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
     1621 ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga
     1681 ggcaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt
     1741 tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg
     1801 aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg actttggggg
     1861 ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata
     1921 tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct
     1981 ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg
     2041 ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac
     2101 tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc
     2161 ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa
     2221 cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa
     2281 ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga
     2341 agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc
     2401 tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg
     2461 atcctccctt tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg
     2521 gtacgtagga attcggcacg agagctgact ctgccactaa gcttgccatg gtgtcttggg
     2581 acaaggctca cctgggtcct aagtatgtgg gcctctggga cttcaaggca cggacagatg
     2641 aggagctgag ctttcaggca ggagacctcc tccatgttac caagaaggag gaactgtggt
     2701 ggtgggccac cctgctggat gcagaaggca aggccttggc tgagggctat gtgcctcaca
     2761 actacctggc tgagaaggaa actgtggagt ctgaaccgtg gttctttggt tgcatctccc
     2821 gctcagaggc catgcacagg ctgcaggctg aggacaactc gaagggtgcc ttcctgatca
     2881 gagtcagcca gaagccagga gcagactatg tcctctctgt ccgggatgct caggccgtgc
     2941 gacattacag gatctggaag aacaacgagg gccggctgca cctgaatgag gcggtatcct
     3001 tctccaatct gtctgagctt gtggactacc ataagaccca gagcctgtct catggcctac
     3061 agctgtccat gccctgctgg aagcacaaaa ctgagccctt gccccactgg gatgactggg
     3121 agaggccgag ggaggagttc acactctgta agaagctggg ggccggctac tttggggagg
     3181 tctttgaagc gctctggaaa ggccaggtcc atgtggctgt gaaggtgatc tctagagaca
     3241 atctcctgca ccagcacacc ttccaggctg agattcaggc catgaagaag ctgcggcaca
     3301 agcacatcct gtcactgtac gctgtggcga ctgcagggga cccggtctac atcatcacgg
     3361 agctcatgcc caaggggaac ctgctgcagc tactgcgtga ctctgatgag aaagccctgc
     3421 ctattttgga gctggtggac tttgcatcac aggttgctga gggcatgtgc tacctggaat
     3481 ctcagaatta catccaccgt gacctggctg caaggaacgt tcttgttaca gagaacaatc
     3541 tctgcaaagt gggggacttt gggcttgcca ggcttgtcaa ggaggacatc tacctttccc
     3601 atgagcacaa tgtcccctac aaatggacag cacctgaggc actttcccga gggcattact
     3661 ccatcaagtc tgatgtctgg tcttttggag ttcttcttca tgaaattttc agcagggggc
     3721 agatgcccta cccaggcatg tccaatcatg aaaccttcct gagggtggat gccggctacc
     3781 gcatgccctg ccctctggag tgcccaccca acatacacaa gctgatgctc agctgctgga
     3841 gcagagaccc caagcagaga ccttgcttca aagacctgtg tgagaaactc acaggtatca
     3901 ccaggtttga gaacctggtc tgagctcgaa ttcgccagca cagtggtcga ccctgtggaa
     3961 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4201 aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     4261 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     4321 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     4381 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     4441 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     4501 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     4561 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     4621 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     4681 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     4741 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     4801 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     4861 aaggagcgca cgaccccatg catcgataaa ataaaagatt ttatttagtc tccagaaaaa
     4921 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     4981 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     5041 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     5101 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     5161 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     5221 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     5281 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     5341 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     5401 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     5461 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
     5521 tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat
     5581 agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5641 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5701 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5761 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5821 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5881 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5941 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6001 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6061 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6121 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6181 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6241 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6301 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6361 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gat
//
