LOCUS       sequence-420226-        6413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Construct for stable expression of mouse wild type Ptk6 (Sik) in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Derry JJ, Richard S, Valderrama Carvajal H, Ye X, Vasioukhin V, 
            Cochrane AW, Chen T, Tyner AL
  TITLE     Sik (BRK) phosphorylates Sam68 in the nucleus and negatively 
            regulates its RNA binding ability.
  JOURNAL   Mol Cell Biol. 2000 Aug;20(16):6114-26. doi: 
            10.1128/MCB.20.16.6114-6126.2000.
  PUBMED    10913193
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.20.16.6114-6126"; journalName: "Mol Cell Biol"; date: 
            "2000-08"; volume: "20"; issue: "16"; pages: "6114-26"
COMMENT     Sequence Label: pBabe-Sik-WT
FEATURES             Location/Qualifiers
     source          1..6413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      24..612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     513..532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     766..783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             913..1382
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(1603..2202)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1686..1706)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2183..2202
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2212..2408)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(2220..2430)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2226..2361
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2454..2474
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(3931..4347)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3947..3963)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3979..4001)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4548..4747
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4812..5281
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5312..5416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5417..5485,5486..6277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5635..5654)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
       61 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      781 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      841 taatgcaact atggccattt aatgtaaata cttaagaaaa aaaaccaaat taattttgat
      901 acatgctgca tgtgaaagac ccccgctgac gggtagtcaa tcactcagag gagaccctcc
      961 caaggaacag cgagaccaca agtcggatgc aactgcaaga gggtttattg gatacacggg
     1021 tacccgggcg actcagtcaa tcggaggact ggcgccccga gtgaggggtt gtgggctctt
     1081 ttattgagct cggggagcag aagcgcgcga acagaagcga gaagcgaact gattggttag
     1141 ttcaaataag gcacagggtc atttcaggtc cttggggcac cctggaaaca tctgatggtt
     1201 ctctagaaac tgctgagggc tggaccgcat ctggggacca tctgttcttg gccctgagcc
     1261 ggggcaggaa ctgcttacca cagatatcct gtttggccca tattcagctg ttccatctgt
     1321 tcttggccct gagccggggc aggaactgct taccacagat atcctgtttg gcccatattc
     1381 agctgttcca tctgttcctg accttgatct gaacttctct attctcagtt atgtattttt
     1441 ccatgccttg caaaatggcg ttacttaagc tagcttgcca aacctacagg tggggtcttt
     1501 cattcccccc tttttctgga gactaaataa aatcttttat tttatcgatg catggggtcg
     1561 tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg
     1621 ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc
     1681 gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc
     1741 ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg
     1801 gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg
     1861 cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat
     1921 gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac
     1981 cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt
     2041 gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg
     2101 gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc
     2161 gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggtaagct ttttgcaaaa
     2221 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     2281 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     2341 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     2401 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     2461 ctgacacaca ttccacaggg tcgaccactg tgctggcgaa ttcgagctca gaccaggttc
     2521 tcatacctgg tgatacctgt gagtttctca cacaggtctt tgaagcaagg tctctgcttg
     2581 gggtctctgc tccagcagct gagcatcagc ttgtgtatgt tgggtgggca ctccagaggg
     2641 cagggcatgc ggtagccggc atccaccctc aggaaggttt catgattgga catgcctggg
     2701 tagggcatct gccccctgct gaaaatttca tgaagaagaa ctccaaaaga ccagacatca
     2761 gacttgatgg agtaatgccc tcgggaaagt gcctcaggtg ctgtccattt gtaggggaca
     2821 ttgtgctcat gggaaaggta gatgtcctcc ttgacaagcc tggcaagccc aaagtccccc
     2881 actttgcaga gattgttctc tgtaacaaga acgttccttg cagccaggtc acggtggatg
     2941 taattctgag attccaggta gcacatgccc tcagcaacct gtgatgcaaa gtccaccagc
     3001 tccaaaatag gcagggcttt ctcatcagag tcacgcagta gctgcagcag gttccccttg
     3061 ggcatgagct ccgtgatgat gtagaccggg tcccctgcag tcgccacagc gtacagtgac
     3121 aggatgtgct tgtgccgcag cttcttcatg gcctgaatct cagcctggaa ggtgtgctgg
     3181 tgcaggagat tgtctctaga gatcaccttc acagccacat ggacctggcc tttccagagc
     3241 gcttcaaaga cctccccaaa gtagccggcc cccagcttct tacagagtgt gaactcctcc
     3301 ctcggcctct cccagtcatc ccagtggggc aagggctcag ttttgtgctt ccagcagggc
     3361 atggacagct gtaggccatg agacaggctc tgggtcttat ggtagtccac aagctcagac
     3421 agattggaga aggataccgc ctcattcagg tgcagccggc cctcgttgtt cttccagatc
     3481 ctgtaatgtc gcacggcctg agcatcccgg acagagagga catagtctgc tcctggcttc
     3541 tggctgactc tgatcaggaa ggcacccttc gagttgtcct cagcctgcag cctgtgcatg
     3601 gcctctgagc gggagatgca accaaagaac cacggttcag actccacagt ttccttctca
     3661 gccaggtagt tgtgaggcac atagccctca gccaaggcct tgccttctgc atccagcagg
     3721 gtggcccacc accacagttc ctccttcttg gtaacatgga ggaggtctcc tgcctgaaag
     3781 ctcagctcct catctgtccg tgccttgaag tcccagaggc ccacatactt aggacccagg
     3841 tgagccttgt cccaagacac catggcaagc ttagtggcag agtcagctct cgtgccgaat
     3901 tcctacgtac caccacactg ggatccggcc ggcgcctaga gaaggagtga gggctggata
     3961 aagggaggat cgaggcgggg tcgaacgagg aggttcaagg gggagagacg gggcggatgg
     4021 aggaagagga ggcggaggct tagggtgtac aaagggcttg acccagggag gggggtcaaa
     4081 agccaaggct tcccaggtca cgatgtaggg gacctggtct gggtgtccat gcgggccagg
     4141 tgaaaagacc ttgatcttaa cctgggtgat gaggtctcgg ttaaaggtgc cgtctcgcgg
     4201 ccatccgacg ttaaaggttg gccattctgc agagcagaag gtaacccaac gtctcttctt
     4261 gacatctacc gactggttgt gagcgatccg ctcgacatct ttccagtgac ctaaggtcaa
     4321 acttaaggga gtggtaacag tctggcccta attttcagac aaatacagaa acacagtcag
     4381 acagagacaa cacagaacga tgctgcagcg ctgcagcaga caagacgcgc ggcttcggtt
     4441 ccaaaccgaa agcaaaaatt cagacggagg cgggaactgt tttaggttct cgtctcctac
     4501 cagaaccaca tatcctgacg gggtcggatt ccacatcgac tcccttcctc aggtcgggcc
     4561 acaaaaacgg cccccaaagt ccctgggacg tctcccaggg ttgcggccgg gtgttcagaa
     4621 ctcgtcagtt ccaccacggg tccgccagat acagagctag ttagctaact agtaccgacg
     4681 caggcgcata aaatcagtca tagacactag acaatcggac agacacagat aagttgctgg
     4741 ccagcttgcc tcccggtggt gggtcggtgg tccctgggca ggggtctccc gatcccggac
     4801 gagcccccaa atgaaagacc cccgctgacg ggtagtcaat cactcagagg agaccctccc
     4861 aaggaacagc gagaccacaa gtcggatgca actgcaagag ggtttattgg atacacgggt
     4921 acccgggcga ctcagtcaat cggaggactg gcgccccgag tgaggggttg tgggctcttt
     4981 tattgagctc ggggagcaga agcgcgcgaa cagaagcgag aagcgaactg attggttagt
     5041 tcaaataagg cacagggtca tttcaggtcc ttggggcacc ctggaaacat ctgatggttc
     5101 tctagaaact gctgagggct ggaccgcatc tggggaccat ctgttcttgg ccctgagccg
     5161 gggcaggaac tgcttaccac agatatcctg tttggcccat attcagctgt tccatctgtt
     5221 cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg cccatattca
     5281 ggctgcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5341 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5401 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5461 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5521 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5581 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5641 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5701 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5761 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5821 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5881 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5941 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6001 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6061 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6121 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6181 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6241 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6301 tatactttag attgatttgc ggccgcaaaa ggatctaggt gaagatcctt tttgataatc
     6361 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac ccc
//
