LOCUS       sequence-420227-       10861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector to express MLV Gag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pLV_CAG_Gag
FEATURES             Location/Qualifiers
     source          1..10861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(450..1241,1242..1310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1073..1092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1311..1415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1490..1509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1681..2060
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2062..2260
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2278..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2505..2630
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3123..3356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3541..3585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3883..4000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4035..4413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4414..4617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4614..4638
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4751..5226
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      5281..5290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5290..5706
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5636..5658
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5674..5690
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             6886..6942
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6943..7638
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFAR
                     TFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAF
                     KTPDADAGEE"
     CDS             7648..7704
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7705..8076
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="AKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV
                     TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE
                     FALRDPAGNCVHFVAEEQD"
     misc_feature    8101..8689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8154..8174)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8572..8583)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8692..8708)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8693..8709)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9214..9394
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9420..9437)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9426..9650
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9696..10124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9783..9802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9993..10014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10150..10166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10150..10166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10163..10185)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10174..10190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10198..10204,10205..10222,10223..10228))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10243..10264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10381..10398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10552..10861,1..279))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10632..10651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
       61 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1441 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1501 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1561 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1621 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     1681 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     1741 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     1801 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     1861 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     1921 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     1981 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2041 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2101 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2161 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2221 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     2281 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2341 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2401 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2461 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2521 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2581 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     2641 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     2701 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     2761 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     2821 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2881 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2941 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     3001 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3061 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3121 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     3181 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     3241 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     3301 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     3361 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     3421 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     3481 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     3541 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     3601 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     3661 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     3721 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     3781 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     3841 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     3901 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     3961 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4021 cagagatcca gtttacattg attattgact agttattaat agtaatcaat tacggggtca
     4081 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     4141 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     4201 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     4261 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     4321 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     4381 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     4441 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4501 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4561 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     4621 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     4681 caccgggacc gatccagcct cccctcgaag cttacatgtg gtaccgagct cggatcctga
     4741 gaacttcagg gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt
     4801 aagttcatgt cataggaagg ggagaagtaa cagggtacac atattgacca aatcagggta
     4861 attttgcatt tgtaatttta aaaaatgctt tcttctttta atatactttt ttgtttatct
     4921 tatttctaat actttcccta atctctttct ttcagggcaa taatgataca atgtatcatg
     4981 cctctttgca ccattctaaa gaataacagt gataatttct gggttaaggc aatagcaata
     5041 tttctgcata taaatatttc tgcatataaa ttgtaactga tgtaagaggt ttcatattgc
     5101 taatagcagc tacaatccag ctaccattct gcttttattt tatggttggg ataaggctgg
     5161 attattctga gtccaagcta ggcccttttg ctaatcatgt tcatacctct tatcttcctc
     5221 ccacagctcc tgggcaacgt gctggtctgt gtgctggccc atcactttgg caaagaattc
     5281 gccaccatgg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
     5341 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
     5401 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
     5461 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
     5521 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
     5581 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
     5641 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
     5701 ggcgccaaac ctaaacctca agttctttct gacagtgggg ggccgctcat cgacctactt
     5761 acagaagacc ccccgcctta tagggaccca agaccacccc cttccgacag ggacggaaat
     5821 ggtggagaag cgacccctgc gggagaggca ccggacccct ccccaatggc atctcgccta
     5881 cgtgggagac gggagccccc tgtggccgac tccactacct cgcaggcatt ccccctccgc
     5941 gcaggaggaa acggacagct tcaatactgg ccgttctcct cttctgacct ttacaactgg
     6001 aaaaataata acccttcttt ttctgaagat ccaggtaaac tgacagctct gatcgagtct
     6061 gtcctcatca cccatcagcc cacctgggac gactgtcagc agctgttggg gactctgctg
     6121 accggagaag aaaaacaacg ggtgctctta gaggctagaa aggcggtgcg gggcgatgat
     6181 gggcgcccca ctcaactgcc caatgaagtc gatgccgctt ttcccctcga gcgcccagac
     6241 tgggattaca ccacccaggc aggtaggaac cacctagtcc actatcgcca gttgctccta
     6301 gcgggtctcc aaaacgcggg cagaagcccc accaatttgg ccaaggtaaa aggaataaca
     6361 caagggccca atgagtctcc ctcggccttc ctagagagac ttaaggaagc ctatcgcagg
     6421 tacactcctt atgaccctga ggacccaggg caagaaacta atgtgtctat gtctttcatt
     6481 tggcagtctg ccccagacat tgggagaaag ttagagaggt tagaagattt aaaaaacaag
     6541 acgcttggag atttggttag agaggcagaa aagatcttta ataaacgaga aaccccggaa
     6601 gaaagagagg aacgtatcag gagagaaaca gaggaaaaag aagaacgccg taggacagag
     6661 gatgagcaga aagagaaaga aagagatcgt aggagacata gagagatgag caagctattg
     6721 gccactgtcg ttagtggaca gaaacaggat agacagggag gagaacgaag gaggtcccaa
     6781 ctcgatcgcg accagtgtgc ctactgcaaa gaaaaggggc actgggctaa agattgtccc
     6841 aagaaaccac gaggacctcg gggaccaaga ccgcagggat ccggcgcaac aaacttctct
     6901 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggagag cgacgagagc
     6961 ggcctgcccg ccatgaagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     7021 gagctggtgg gcggcggaga gggcaccccc gagcagggcc gcatgaccaa caagatgaag
     7081 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     7141 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     7201 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     7261 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     7321 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     7381 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     7441 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     7501 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     7561 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gaccccggat
     7621 gcagatgccg gtgaagaagg atccggcgca acaaacttct ctctgctcaa acaagccggc
     7681 gacgtcgaag agaatcctgg gccggccaag ttgaccagtg ccgttccggt gctcaccgcg
     7741 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     7801 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     7861 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     7921 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     7981 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     8041 aactgcgtgc acttcgtggc cgaggagcag gactgagaat tcgatatcaa gcttatcggt
     8101 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8161 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8221 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8281 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8341 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8401 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8461 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8521 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8581 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8641 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     8701 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     8761 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     8821 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8881 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     8941 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     9001 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     9061 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     9121 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     9181 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     9241 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9301 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9361 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     9421 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     9481 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     9541 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     9601 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     9661 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     9721 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9781 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9841 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9901 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9961 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    10021 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    10081 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtagcttggc
    10141 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    10201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    10261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gtcagctgca
    10321 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10381 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10441 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10861 t
//
