LOCUS       sequence-420235-       21931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A plasmid containing a 11,944 bp fragment of the P. tricornutum 
            chloroplast genome. The fragment is flanked by SapI recognition 
            sites, allowing it to be released from the cloning vector pSAP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21931)
  AUTHORS   Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
  TITLE     Design and assembly of the 117-kb Phaeodactylum tricornutum 
            chloroplast genome.
  JOURNAL   Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.
  PUBMED    38114089
REFERENCE   2  (bases 1 to 21931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670."
COMMENT     Sequence Label: pCHAP8
FEATURES             Location/Qualifiers
     source          1..21931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..756
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    759..1009
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    1171..1674
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        1728..1875
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             1876..2550
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     CDS             2946..4121
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4121..5092
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    6113..6511
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(6529..6562)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     6630..6649
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(6729..7004)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     6842..6864
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6856..6873
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6857..6873
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6880..6899
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6880..6898
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6901..6957
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7000..7016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7000..7016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7013..7035)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7024..7040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7048..7054,7055..7072,7073..7078))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7093..7114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(7334..7993)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7908..7927
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7994..8096)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    18199..18210
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             18586..18597
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      20932..21546
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      21023..21554
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      21622..21841
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
ORIGIN
        1 atggcggaaa cagcggttat caatcacaag aaacgtaaaa atagcccgcg aatcgtccag
       61 tcaaacgacc tcactgaggc ggcatatagt ctctcccggg atcaaaaacg tatgctgtat
      121 ctgttcgttg accagatcag aaaatctgat ggcaccctac aggaacatga cggtatctgc
      181 gagatccatg ttgctaaata tgctgaaata ttcggattga cctctgcgga agccagtaag
      241 gatatacggc aggcattgaa gagtttcgcg gggaaggaag tggtttttta tcgccctgaa
      301 gaggatgccg gcgatgaaaa aggctatgaa tcttttcctt ggtttatcaa acgtgcgcac
      361 agtccatcca gagggcttta cagtgtacat atcaacccat atctcattcc cttctttatc
      421 gggttacaga accggtttac gcagtttcgg cttagtgaaa caaaagaaat caccaatccg
      481 tatgccatgc gtttatacga atccctgtgt cagtatcgta agccggatgg ctcaggcatc
      541 gtctctctga aaatcgactg gatcatagag cgttaccagc tgcctcaaag ttaccagcgt
      601 atgcctgact tccgccgccg cttcctgcag gtctgtgtta atgagatcaa cagcagaact
      661 ccaatgcgcc tctcatacat tgagaaaaag aaaggccgcc agacgactca tatcgtattt
      721 tccttccgcg atatcacttc catgacgaca ggatagtctg agggttatct gtcacagatt
      781 tgagggtggt tcgtcacatt tgttctgacc tactgagggt aatttgtcac agttttgctg
      841 tttccttcag cctgcatgga ttttctcata ctttttgaac tgtaattttt aaggaagcca
      901 aatttgaggg cagtttgtca cagttgattt ccttctcttt cccttcgtca tgtgacctga
      961 tatcgggggt tagttcgtca tcattgatga gggttgatta tcacagttta ttactctgaa
     1021 ttggctatcc gcgtgtgtac ctctacctgg agtttttccc acggtggata tttcttcttg
     1081 cgctgagcgt aagagctatc tgacagaaca gttcttcttt gcttcctcgc cagttcgctc
     1141 gctatgctcg gttacacggc tgcggcgagc atcacgtgct ataaaaataa ttataattta
     1201 aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa
     1261 aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc
     1321 ttttaccttg ctctacctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt
     1381 agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat
     1441 atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga
     1501 aattttttaa acctttgttt attttttttt cttcattccg taactcttct accttcttta
     1561 tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca
     1621 aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccagatg
     1681 gcagtagtgg aagatattct ttattgaaaa atagcttgtc accttacgta caatcttgat
     1741 ccggagcttt tctttttttg ccgattaaga attaattcgg tcgaaaaaag aaaaggagag
     1801 ggccaagagg gagggcattg gtgactattg agcacgtgag tatacgtgat taagcacaca
     1861 aaggcagctt ggagtatgtc tgttattaat ttcacaggta gttctggtcc attggtgaaa
     1921 gtttgcggct tgcagagcac agaggccgca gaatgtgctc tagattccga tgctgacttg
     1981 ctgggtatta tatgtgtgcc caatagaaag agaacaattg acccggttat tgcaaggaaa
     2041 atttcaagtc ttgtaaaagc atataaaaat agttcaggca ctccgaaata cttggttggc
     2101 gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg tcaatgatta cggcattgat
     2161 atcgtccaac tgcatggaga tgagtcgtgg caagaatacc aagagttcct cggtttgcca
     2221 gttattaaaa gactcgtatt tccaaaagac tgcaacatac tactcagtgc agcttcacag
     2281 aaacctcatt cgtttattcc cttgtttgat tcagaagcag gtgggacagg tgaacttttg
     2341 gattggaact cgatttctga ctgggttgga aggcaagaga gccccgaaag cttacatttt
     2401 atgttagctg gtggactgac gccagaaaat gttggtgatg cgcttagatt aaatggcgtt
     2461 attggtgttg atgtaagcgg aggtgtggag acaaatggtg taaaagactc taacaaaata
     2521 gcaaatttcg tcaaaaatgc taagaaatag gttattactg agtagtattt atttaagtat
     2581 tgtttgtgca cttgccgatc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2641 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2701 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcatttt
     2761 acacaggagt ctggacttga cgctagtgat aataagtgac tgaggtatgt gcgcttctta
     2821 tctccttttg tagtgttgct cttattttaa acaactttgc ggttttttga tgactttgcg
     2881 attttgttgt tgctttgcag taaattgcaa gatttaataa aaaaacgcaa agcaatgatt
     2941 aaaggatgtt cagaatgaaa ctcatggaaa cacttaacca gtgcataaac gctggtcatg
     3001 aaatgacgaa ggctatcgcc attgcacagt ttaatgatga cagcccggaa gcgaggaaaa
     3061 taacccggcg ctggagaata ggtgaagcag cggatttagt tggggtttct tctcaggcta
     3121 tcagagatgc cgagaaagca gggcgactac cgcacccgga tatggaaatt cgaggacggg
     3181 ttgagcaacg tgttggttat acaattgaac aaattaatca tatgcgtgat gtgtttggta
     3241 cgcgattgcg acgtgctgaa gacgtatttc caccggtgat cggggttgct gcccataaag
     3301 gtggcgttta caaaacctca gtttctgttc atcttgctca ggatctggct ctgaaggggc
     3361 tacgtgtttt gctcgtggaa ggtaacgacc cccagggaac agcctcaatg tatcacggat
     3421 gggtaccaga tcttcatatt catgcagaag acactctcct gcctttctat cttggggaaa
     3481 aggacgatgt cacttatgca ataaagccca cttgctggcc ggggcttgac attattcctt
     3541 cctgtctggc tctgcaccgt attgaaactg agttaatggg caaatttgat gaaggtaaac
     3601 tgcccaccga tccacacctg atgctccgac tggccattga aactgttgct catgactatg
     3661 atgtcatagt tattgacagc gcgcctaacc tgggtatcgg cacgattaat gtcgtatgtg
     3721 ctgctgatgt gctgattgtt cccacgcctg ctgagttgtt tgactacacc tccgcactgc
     3781 agtttttcga tatgcttcgt gatctgctca agaacgttga tcttaaaggg ttcgagcctg
     3841 atgtacgtat tttgcttacc aaatacagca atagcaatgg ctctcagtcc ccgtggatgg
     3901 aggagcaaat tagggatgcc tggggaagca tggttctaaa aaatgttgta cgtgaaacgg
     3961 atgaagttgg taaaggtcag atccggatga gaactgtttt tgaacaggcc attgatcaac
     4021 gctcatcaac tggtgcctgg agaaatgctc tttctatttg ggaacctgtc tgcaatgaaa
     4081 ttttcgatcg tctgattaaa ccacgctggg agattagata atgaagcgtg cgcctgttat
     4141 tccaaaacat acgctcaata ctcaaccggt tgaagatact tcgttatcga caccagctgc
     4201 cccgatggtg gattcgttaa ttgcgcgcgt aggagtaatg gctcgcggta atgccattac
     4261 tttgcctgta tgtggtcggg atgtgaagtt tactcttgaa gtgctccggg gtgatagtgt
     4321 tgagaagacc tctcgggtat ggtcaggtaa tgaacgtgac caggagctgc ttactgagga
     4381 cgcactggat gatctcatcc cttcttttct actgactggt caacagacac cggcgttcgg
     4441 tcgaagagta tctggtgtca tagaaattgc cgatgggagt cgccgtcgta aagctgctgc
     4501 acttaccgaa agtgattatc gtgttctggt tggcgagctg gatgatgagc agatggctgc
     4561 attatccaga ttgggtaacg attatcgccc aacaagtgct tatgaacgtg gtcagcgtta
     4621 tgcaagccga ttgcagaatg aatttgctgg aaatatttct gcgctggctg atgcggaaaa
     4681 tatttcacgt aagattatta cccgctgtat caacaccgcc aaattgccta aatcagttgt
     4741 tgctcttttt tctcaccccg gtgaactatc tgcccggtca ggtgatgcac ttcaaaaagc
     4801 ctttacagat aaagaggaat tacttaagca gcaggcatct aaccttcatg agcagaaaaa
     4861 agctggggtg atatttgaag ctgaagaagt tatcactctt ttaacttctg tgcttaaaac
     4921 gtcatctgca tcaagaacta gtttaagctc acgacatcag tttgctcctg gagcgacagt
     4981 attgtataag ggcgataaaa tggtgcttaa cctggacagg tctcgtgttc caactgagtg
     5041 tatagagaaa attgaggcca ttcttaagga acttgaaaag ccagcaccct gatgcgacct
     5101 cgttttagtc tacgtttatc tgtctttact taatgtcctt tgttacaggc cagaaagcat
     5161 aactggcctg aatattctat ctgggcccac tgttccactt gtatcgtcgg tatgataatc
     5221 agactgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc
     5281 ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg tatcgtcggt
     5341 ctgataatca gactgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg
     5401 accatggtcc cactcgtatc gtcggtctga ttattagtat gggaccacgg tcccactcgt
     5461 atcgtcggtc tgattattag tctggaacca cggtcccact cgtatcgtcg gtctgattat
     5521 tagtctggga caacggtccc actcgtatcg tcggtctgat tattagtctg ggaccacggt
     5581 cccactcgta tcgtcggtct gataatcaga ctgggaccat ggtcccactc gtatcgtcgg
     5641 tctgattatt agtctgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg
     5701 aaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg
     5761 tatcgtaggt ctgattatta gtctgggacc acgatcccac tcgtgttgtc ggtctgatta
     5821 tcggtctggg accacggtcc cacttgtatt gtcgatcaga ctatcagcgt gagactacga
     5881 ttccatcaat gcctgtcaag ggcaagtatt gacatgtcgt cgtaacctgt agaacggagt
     5941 aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc ttatccacaa
     6001 cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc cggcacgtta
     6061 accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct cggacatgag
     6121 gttgccccgt attcagtgtc gctgatttgt attgtctgaa gttgttttta cgttaagttg
     6181 atgcagatca attaatacga tacctgcgtc ataattgatt atttgacgtg gtttgatggc
     6241 ctccacgcac gttgtgatat gtagatgata atcattatca ctttacgggt cctttccggt
     6301 gatccgacag gttacggggc ggcgacctcg cgggttttcg ctatttatga aaattttccg
     6361 gtttaaggcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa aataccctct
     6421 gaaaagaaag gaaacgacag gtgctgaaag cgagcttttt ggcctctgtc gtttcctttc
     6481 tctgtttttg tccgtggaat gaacaatgga agtccgagct catcgctaat aacttcgtat
     6541 agcatacatt atacgaagtt atattcgatg cggccgcaag gggttcgcgt cagcgggtgt
     6601 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     6661 ccatatgcgg tgtgaaatac cacacagatg cgtaaggaga aaataccgca tcaggcgcca
     6721 ttcgccattc agctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     6781 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6841 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
     6901 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttgag
     6961 tattctatag tctcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7021 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7081 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7141 ctagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg aaccccttgc
     7201 ggccgcccgg gccgtcgacc aattctcatg tttgacagct tatcatcgaa tttctgccat
     7261 tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg cacaaataac
     7321 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     7381 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     7441 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     7501 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     7561 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     7621 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     7681 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     7741 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     7801 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     7861 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     7921 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     7981 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     8041 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     8101 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     8161 tttattctgc gaagtgatct tccgtcacag gtatttattc gcgataagct catggagcgg
     8221 cgtaaccgtc gcacaggaag gacagagaaa gcgcggatct gggaagtgac ggacagaacg
     8281 gtcaggacct ggattgggga ggcggttgcc gccgctgctg ctgacggtgt gacgttctct
     8341 gttccggtca caccacatac gttccgccat tcctatgcga tgcacatgct gtatgccggt
     8401 ataccgctga aagttctgca aagcctgatg ggacataagt ccatcagttc aacggaagtc
     8461 tacacgaagg tttttgcgct ggatgtggct gcccggcacc gggtgcagtt tgcgatgccg
     8521 gagtctgatg cggttgcgat gctgaaacaa ttatcctgag aataaatgcc ttggccttta
     8581 tatggaaatg tggaactgag tggatatgct gtttttgtct gttaaacaga gaagctggct
     8641 gttatccact gagaagcgaa cgaaacagtc gggaaaatct cccattatcg tagagatccg
     8701 cattattaat ctcaggagcc tgtgtagcgt ttataggaag tagtgttctg tcatgatgcc
     8761 tgcaagcggt aacgaaaacg atttgaatat gcattcagga acaatagaaa tcttcgtgcg
     8821 gtgttacgtt gaagtggagc ggattatgtc agcaatggac agaacaacct aatgaacaca
     8881 gaaccatgat gtggtctgtc cttttacagc cagtagtgct cgccgcagtc gagcgacagg
     8941 gcgaagcccg ctcttcgcag tcactccgct ttggtttaag aattactctt tctctaagtt
     9001 tatttgattt ttttcagtaa atttttaaaa tttcagcatt tatactgaac attataaatt
     9061 agaaacaatt acttatgtca aatactcaat cggttacagc tgtagcgaaa tacattcgca
     9121 tgtcacctca taagatcaga cgggttttag atcaaatccg tggtcgttct taccaagaag
     9181 ccctaatgat tttagaattt ttaccgtatg atgctggtgg gccgatttgg caagttattc
     9241 actccgcagc tgcgaatgct aaacacaata gtggtttaga taaaaaaaaa ctaattatcg
     9301 ataaggtttt tgccgacgaa ggaccaaagt taaagcgaat tcgcccaaga gctcaaggaa
     9361 gggcatacaa aatattaaaa cccacttgtc atattacagt ggttatgaag gccattgact
     9421 aaaaaactaa ataataaatt tgatttaaaa ttaaaaggat taactctatg gggcaaaaaa
     9481 cacatccttt aggatttaga ttaggtatta cacaagaaca taaatcaacg tggtatgcta
     9541 attttaatca atatgccaac attttgaagg aggatgataa aattcgaaca tatattcata
     9601 ctatttctaa agcaaatagt attgcaaacg ttcgaattaa tcgtaacggg ctaaatgatc
     9661 agattcaact aaatattgaa actggaaaac caggtatctt agttggtgac ttaggtgctg
     9721 gtcttgagac attgttaaat aatgttaaaa aatttttacc agcaaatcgt caattgacaa
     9781 tcaatgtttt ggaagttgaa aaagtcgatt tagatgcttc acttttagct gacttagttg
     9841 ccgaacaatt agagaaacgt attgcatttc gtcgcgcaat tcgtgaagca ctacaacgag
     9901 cacaaaaaca gaatgtaaat ggtattaaaa ttcaagtctc tggtcgttta aatggcgctg
     9961 aaattgctag aagcgaatgg attcgtgaag ggcgagttcc tttacaaaca ttacgagcag
    10021 atattgatta cgcaacacaa gaagccaata caatctatgg cgttttagga atcaaagttt
    10081 ggttatttaa aagtgaaatt ttatcaaagt aaaaaaattt atattcttat taaattaatt
    10141 tgttatgtta agtccaaaaa gaacaaaata tagaaaatat catcgtgggc gtatgagagg
    10201 ggcaaaaacg cgtggaaatg aaatttgctt tggaaatttt gggttacagg cacttgaacc
    10261 gacttggatt acctcacgtc aaattgaagc tgcacgtaga acaattactc gttatacgaa
    10321 acgtggggca aaattatgga tacgaatttt tcctgacaaa actgtaactg cgcgagctgc
    10381 agaatcaaga atgggttcag gtaaaggggc ggtagattat tgggttgccg tagtcaagcc
    10441 aggaacaatt atttttgaaa ttggttcagt accagaagaa attgcaaaga ctgcattaaa
    10501 tttggctgct tataaattac caattaaaac aaaatttatt attaaagata atattagtta
    10561 aaaattatga gtttacctgc atttactgag attagttcat tttcaaatac agaaatttca
    10621 gtagcaatta ttgaaactga aaatcaatta tttaatcttc gttttaaaaa agcgacgcgt
    10681 caaagcttta aatcccatga aattaaaaat gcaaaacgtc gtttagcaca attaaaaaca
    10741 cttttaagtt taagattgga aaatctggat caaaaagatg ataatttaat taatactcta
    10801 attacgaatt aagatcaaat gacaaaatta aatactattc aatgtaagat taaggagttt
    10861 tagatgccag taaaacaaca aattggtatt gtaattagta ataaaatgca aaaaacaatt
    10921 gtggtaaaga ttgaaaatcg atacccacac ccaatgtatt ctaaaacact aataaaaaca
    10981 aaaaaatact tagctcatga tgagcttggc gaatgtaata ttggtgatca agttttagtg
    11041 gaagaatgcc gcccattaag taaaagaaaa cgctggaagt taatcaaaat tctttcaaaa
    11101 tcatcgttaa ttaattaaat gagattttta tgatttatcc tcaaacaatg ttaacagtag
    11161 ccgataatac gggtgctaaa aaagttatgt gtattcgggt attaggtggc aacaagaaat
    11221 atgcaaaaat tggtgataca attattgcag ttgttaaaga agccttacca aatatgcctg
    11281 taaaacgttc cgatgtggta agagccgttg ttgtgcgaac aaagaaaagt attcgtcgac
    11341 aagatggtat gtatattcga tttgatgata acgcggctgt aattgtgaat atggataata
    11401 atcctcgtgg gactcgagtt tttggacctg ttgctcgtga aattcgcgat aaaaattatt
    11461 caaaaattgt atccttagcg ccggaggttt tataaatgcc acaaaaacaa aaagtaaatg
    11521 ttaaaattgg cgattcggtg acagttattt cgggttttca caaaaatgag actggtgaag
    11581 ttcttaaaat taatcgtaag aatggaaaaa taatagtaaa aggaattaat tttaagttta
    11641 aacacgtgaa acccactact gaaaatgaaa ttggtgaaat taaacagttt gaagcaccac
    11701 ttcatcattc aaatgttaaa ttaaatttaa aaaaagtatt gtaatatgct aaatcaacat
    11761 tctctagagg aaattgctga aattcataat ttactgggca atattaaaca ggaatatgaa
    11821 atcgggatca agcccctttt aataaaaaac agcccaaaac tgtttaaaaa tccgcatact
    11881 gtacctaaac taaaaaaaat tcagattaat cgcggcttag ggttagctgc tcaaaatact
    11941 aatctattaa aaaaaaacat cgaagagttt gagaaaattg ctggtcaaaa accattaatt
    12001 acaagatcta aaaaagctat tgctggtttt aaaattcgcg aagatatgga attaggacta
    12061 agtgtgactc tacgcggtga aaaaatgtat acatttttaa ccaaactatt attttttacg
    12121 tttgcgcaaa ttcgtgattt tcgtggtttg tcattacgaa gttttgataa agctggcaat
    12181 tatacactag gtctgaaaga acaattaatt tttccagaaa ttgattacga tgatgttgac
    12241 caaattcaag gttttacaat taatataatt ctagaacatg gatcgccaaa atatagagca
    12301 gaatcaatag ataagatttt aaatggtatg attttattta aatttttacg gtttccctta
    12361 aatgattgtg gttattacga taagtatgaa gcattctctg atataaatcg taattgggat
    12421 aaaaaaaggc atttaaaacg taaacgctgg tcacaagaat aataataaat atattgtaga
    12481 aggagaaaat tgtggtaaac gatacgattt cagatatgtt aacacggatc cgaaacgcga
    12541 atatggtaaa acatcaaata gttcaaatcc ctgctagtaa gatgtcttta gcaattgcag
    12601 aaattttaaa gcaagaagga tttattggag attttgaaac ttacagtgaa aatgagaaga
    12661 aatatttgtt aatctcatta aaatatgttg gaaaactgag aaagcctgtt atttgtaaaa
    12721 ttgaacgagt gagcaaacca ggattacggg tgtattcaaa ctcaaaaaaa ttaccaagaa
    12781 tattagataa tttaggcatt gcaattattt cgacatcaaa aggagtaatg acaaatctaa
    12841 aggcaaaaga acttgggatt ggtggcgaag ttttgtgtta tatttggtaa acattaggga
    12901 gtttctaaaa tgtcacgtat aggaaaatta ccgattacta taccagaaaa tgtagatatt
    12961 aattacaatg attcagaaat tacagttaaa gggaagtttg gcacgttaca aacacaaatt
    13021 cctactgtca ttaaaattac tgaaagtgat aatattttaa ccgttggttt aagtgaacaa
    13081 acaagaagtg ttcgggcatt acatggttta tatagaactt taattaacaa tatggtaatt
    13141 ggagtatcag aacaatttga gataatactt gaattgaaag gtgttggata tcgagcagca
    13201 gttcaaaata acgaaattat tttaaattta ggatatagtc atccggttaa tattaaaatt
    13261 cctaacatta tttcggttga ggttgttcaa aatacaacca taaatttaaa atcatgtgat
    13321 aaagaattgc ttggcttatt tgcagcaaat attagagctt ggcgtcaacc agaaccatac
    13381 aaaggaaaag gaattctata caaaggtgaa caaattattc gcaaagctgg taaatctgca
    13441 aagtaaaatt gaaatggttg tatgactgtt tttctaattt gatagaaaat cattcttttt
    13501 gattatagaa atgtctatcg aatgcttacc gttttaaaaa attaaaaatt ataaatatga
    13561 aattttcaca acgaactttg ttgaaattaa aaaataaaaa taaaaaatta agaccaaata
    13621 aatataaaaa attaaaacgt gaaactttgc gtggtcgaat caagggaact gttgaaagac
    13681 caaggttgtc agtataccgc tcaaatgaaa acatttatgc gcaaataatt gatgatacta
    13741 attcaaaaac tttaatttct tgttcaactt tagatcgatc tattaaactg gtgatttcga
    13801 cgggtcgaac ttgtgatgct tcaagattaa tgggcgaaaa attagccaaa ttaagtttaa
    13861 aaaaaaatat tacaaaaatt gtttttgatc gaggtcctta cttataccat ggccgtataa
    13921 aagcagtggc agatggggca agagctggcg gattacaatt ttaaattcaa atttgtaaat
    13981 aaatatacaa gttaaatatc ttctatgagt agtgatttaa aaaaaataaa taaagtgaag
    14041 aaaaattctc gacgcatttc aaacacacaa gagcctaaat tagtcgaaag gttaataaag
    14101 attagtcgag tttctaaagt aactaaaggt gggaaaaaat taagttttcg cgcaattgta
    14161 gtggttggag atgaaaatgg taaagttggg gtaggtgttg ccaaagctga tgatgttgtt
    14221 aatgcgttta aaaaagcaaa aactgatggt cgcaaaaatt taattgaact tcctattacg
    14281 aaagcattaa gtattcctca taatgttgcg ggaaattttg gagcctgtaa agtaattatg
    14341 cgaccatcaa tcgaaggttc tggtgtaatc gctggtggag cagttagaat agttttagaa
    14401 gttgcaggtg ttaaaaatgt aattgcgaaa caattaggat caaataactt attaaataat
    14461 gctagggcgt ccgtttgtgc tttacaaaat ttaacaacga aagctcaagt agcaaaaaaa
    14521 agaaatttag attagatata attcagcaaa tatcaattaa aaataaaatt gtgaaagtta
    14581 acaaagaaat tagagacatt atattaaaac gtttattagt gactctggga gtactgttac
    14641 taattcgcgt aggaacgttt ttacccgttc caggaattaa ccatagtgat ttagcatttt
    14701 atattcagag tcactcggtt acaaagagtt tagttagtac attttcaggt gatgatacgt
    14761 ttgtaattgg cttgtttact ttaaatatat tcccatatat caatgcttca attttagtgc
    14821 aattaatttt gggcttttct ccaaagttgt ctaaactaca aaaagaaggt gatttagagg
    14881 gtagacggtc aattaatcga ttaacacgtc tgattactct aatgtttgca cttattcaaa
    14941 gcgtaagctt agcactttat ctaaagcaaa tactatttaa ttggaactat agcctagcaa
    15001 ttgaaattgg gttgtggtta acgaccggag caatgattgt tttatggcta agtgaattaa
    15061 ttactgacta tggtttaggt aatggtgctt cattacttat ttatacaaat attatttcaa
    15121 gcttaccaaa tttaagtaaa acaatactta gtgaaaacag tcaaaatttt acacttggat
    15181 cagaaatcgg aattgtttta ttaattttta cttcactata tggaattgtt tttttacaag
    15241 aaggtgttcg aattattcca cttatttctt ctaaacaatt gaatcaatct tctttacagg
    15301 attcagtaac aagcaataat tatattccat taagatttaa tcaagctggc gtaatgccaa
    15361 taattttgac aactacaatt ttagtagttc caaattatgt aaataactta ggcttattac
    15421 ccaaaattga tcttccgata aacttcgaat cactaaattt tgtttattgg attgggtatt
    15481 ttgtattaat tttagtgttt agttcatttt attccacgat tgttttaaat ccgaaagata
    15541 tttcggacca attacagaag atggccgtaa ctattccagg cattcgacca ggtattcaaa
    15601 caacttttta tttaaaacaa gtaatgaaac gaattacttt attagggaca acaatgcttg
    15661 ctacattagc tacgatacca aattttgtgg agtcaacttt aagtattaca agtttaaatg
    15721 gtctaagtac gacttccctg ttaattttag caggtgtagt tttagactta gttcgtgagg
    15781 tgaaaaatat ttactattcg aatgtttata acaatatgta tcaataaaat aaaaattact
    15841 tttaaaaggt taaaatgaaa gttcgtcctt cagtcaaaaa aatgtgtgat aaatgtcgag
    15901 taattaaacg acatggtaaa attatggtaa tttgttcaaa tccaaaacat aaacaacgtc
    15961 aaggataaaa ttttaaagga gtaaaaaatg gtcagattag ttggtgtgga tttaccaaga
    16021 aataaaagaa ttgcttatgc tcttacttat attcatggaa tcggtcttac ttctgcaaga
    16081 aaaattattg agattgcaaa tattaaccca gaaacaagaa ctgttgattt aacaacagaa
    16141 gaaaccgttg ctttacgtca aactttagaa gaaagtgatt taaaattgga aggtgactta
    16201 agaagattta atggtttaaa tattaaacga ttaaatgaga taaactgtca tagggggaaa
    16261 agacaccgta ataatttacc tgtgcgcggt caacgaactc gaactaatgc tcgttcaaga
    16321 cgtgggtcga aaaaaactgt tacgggtaag aaaaaatagt tttctactaa tattttagat
    16381 ttatttggaa tttttatatg gcacaaacaa cgagaaaatc aacaattaga aaagagaaaa
    16441 atagttttac aagtggggtt gttcatattc aatcaacttt taacaataca attgtgacga
    16501 ttactaattt aactggagat acaatttcct gggcatctgc tggaagttca ggctttaaag
    16561 gtgccagaaa gagtacacca tttgcagctc aaacagctgc tgaaaaagct gccctagaag
    16621 ctttaagtac gggtatgaaa actgttgaaa ttttagtaaa agggcaagga tcaggacgtg
    16681 agacagccat tcgagcaatt gaaggtgcag gttttgatat tatttctata caagatataa
    16741 catcagttcc tcataatggt tgtcgaccac caaaaagacg tcgcgtttag aagttctatt
    16801 tctaataaat taaattatga ataacatttc tataaagtgc cttaagtctg agaagattca
    16861 atcaggtgct tgtcatggtc aatttttaat aaattctttg aatccaggtc aaggtataac
    16921 cattggaaac caattaagac gagtactttt aggtgattta ggtggtgtag ctatttcagc
    16981 agtacgaatt gctggtataa cacatgaatt ttcaacaatt ccgggtgttc gagaagatat
    17041 tctcgaaatt ttgttaaatt tgaagggaat tatttgcaca agtcaaatac aagacactca
    17101 atttggacat ttaaaagttc aaggaccaag cgttgtaact gcagatttaa ttcaattacc
    17161 acccggagta gaaattatta atccaaatca ttatattgcc acaatatcga cgtctaatat
    17221 tcttgagatt gaatttaaat ttgagtatgg aagcggctat catttagcgt ctcaaaattt
    17281 tgtagaggaa gatgaaaatt atctacaact tgatacaatt ttcatgccag ttcaaaaagt
    17341 agactttaaa atcgagaata tttatgatgc tacaaataat attagcgaac gattatttct
    17401 agatatatgg actaatggta gtatttcacc aaatgatgct ctcgaatctg ctgctcaagt
    17461 tattattgac ttatttactt tattgattaa caataaaaat ataaataata ataatcgact
    17521 agagttaaag ccagagacaa ttagtattga accatatact aatattgcaa ttgaagaatt
    17581 acaattatct gttcgggcat ataactgttt gaaaaaagct caaataaata ccgttggtga
    17641 cttattacaa tactcaccag aaaaattaca agaactgaaa aattttggtc gaaaatctgc
    17701 tgatgaagtt ttttctacat taaaaaataa attaggcatt attctaaaat aactaatagt
    17761 tttatttggg attttttaat tcgtcagcat ttttcattat taatttaaaa aaaattatga
    17821 attcattaaa taaaacattt ttaccaacta aaaattataa aaatcgaaat tggtttatta
    17881 ttgattgtaa aaatcaaaaa ctaggtcgac ttgcaacaat tattgtttca ttattaaaag
    17941 gaaaaacaaa accacattat tatccatcta ttgacatcgg tgattatgtt attctactaa
    18001 atgctgattc aattatcatc aatgaaaaca gcaagcatta tattgttaat aagcccggta
    18061 aaccgggtag ttcattaaag attaaaaatg tttctgattg tcttcctaaa tttactatcg
    18121 aacgtgctgt caaaggtatg ttgtctgaaa cagaaacaaa aagattgatg cgtcgattaa
    18181 acatttatag tgagagtagt catcctcatc aggctcaaaa acccatcgaa attaatattt
    18241 cgaactttca ttctgataat cagtttaata cgcaaatggt ttaaataata caaaagtata
    18301 attataaata ttgtaaattt atggattcta aaaaagagtt aattttcaag aaagagaaca
    18361 tcgggctcgg aaaaagaaaa cagtcaattg ctcgcgtttt cttacttccg ggcgacggta
    18421 aaattattat taataaaact tcgggcaata aatatctaca atacaatgat aattatttaa
    18481 atactgtttg gtctccattg gaaaaactaa acttagagaa acaatttgat attgtagctt
    18541 tagttcaagg tggtggatta acagggcaag ctcaagcaat tcaattggga gttgcaagat
    18601 tactatgtac gatggataaa gaaaatcgga gtattttaaa accatttgga ttcttaacac
    18661 gtgattcaag aattaaagaa cgtaaaaaat atggtttacg aaaagcacga aaagctccac
    18721 aatattcaaa acgttaaata atatatttgt ttgaaaaaaa tatgccaaaa tctgatatac
    18781 atccaacctg gtttgaaaat acaaaagttt tatgtgatgg taaaccactt tgtttaatcg
    18841 gttcaacaaa aagtgaacta caaattgata tgtggttagc taatcatccg ttttatacaa
    18901 attcacaagt tcttattgat agtgaaggtc gagttgaaaa atttatgaaa aaatatcgtt
    18961 tagatataac tgaacaataa ttaaacaatt aatttatatg taaatatagt aatttatgcc
    19021 aacgattcaa caattagtcc gttcaagacg tatacaaatt aaaaaaaaaa caaaatcacc
    19081 agcacttgtt aattgtccac aacgacgtgg agtttgtact cgcgtttaca caacaacacc
    19141 aaaaaaaccg aactcagcaa ttcgaaaagt agctcgtgta agattaactt caggctttga
    19201 agttacagcc tacattcctg gagttggtca taatttacaa gagcattcag ttgttttaat
    19261 acgtggaggt cgagttaaag atttaccagg ggttcgctat catattatcc gtggtgcttt
    19321 ggatactggt ggtgtgaaag gtcgtacaca aggccgatca aagtatggtg ttaaaaagcc
    19381 aaaagctgaa aaataaacaa aaatattaat tatttgactc aaaagtgagt acaagttctc
    19441 ttctttaatc acttttgttt cagataattc ggtaaaaaat ttataaatta tattatgtct
    19501 cgtcgaaata tctcaaaaaa acgttttcca gaaaccgatg caatttataa tagttattta
    19561 gttagtttat taatttcaag aattttaaaa tctggtaaaa aaacaattgc caagaaaatt
    19621 gtttatcaag catttgatat tattaagaaa aaaacaaatg aagatccatt aacagttttt
    19681 gaaaaagcaa ttcggaatgc tagtccaatt gtcgaagtaa aagcacgtag agtaggtggt
    19741 tcaacatatc aagtaccagt agaagttact ggttttcgag ctactaattt atcgttaaga
    19801 tggattatac gatatggaga tcaacgtgtt ggccgaagca tggctattaa actagctaat
    19861 gaaattattg acaccgctaa cgatatcggt aatactatta aaaagaaaga agaaactcat
    19921 aaaatggcag aagctaataa agcctttgca catttccgat attaactctt tcattaatta
    19981 atctcgctaa aagcttaaaa atataaatat acttttaaaa aaattttaag gaattttata
    20041 atggcacgtg aaaaatttga acgtacaaaa ccacatgtta atattggtac tattggtcac
    20101 gtagatcatg gaaagacaac tttaactgca gcaatcactg caactttagc cctagatggt
    20161 ggtgcacaag caaaagctta cgctgatatt gatggtgctc ctgaagaacg tgcacgtggt
    20221 attactatta atacagcaca cgttgaatat gaaacaaaag atcgtcatta tgcacatgta
    20281 gattgtccag gtcacgctga ctacgttaaa aatatgatta ctggtgctgc acaaatggat
    20341 ggtgcaatct tagtagtttc tgctgccgat ggtccgatgc ctcaaacacg tgagcatatc
    20401 ttattatcaa aacaagttgg agttccaaac attgttgtat ttttaaataa agaagaccaa
    20461 gtagatgatg aggaattatt agaattagta gaattagaag ttcgtgaatt actttctgct
    20521 tatgatttcc caggtgatga tattccaatc tgtcccggat cagctttaca agctatcgaa
    20581 gcaattacag caaatccaac tgtaaaacgt ggtgataata aatgggttga taaaatttat
    20641 gcattaatgg atgcggtaga cgaatatatt cctaccccgg agcgtgatgt agaaaaaaca
    20701 ttcttaatgg caattgaaga tgtgttttca atcacgggcc gtggtacagt tgcgacagga
    20761 cgtatcgaac gtggtgtagt aaaagttggt gaaaacgttg aaatcgtggg tgttaccgat
    20821 acacaaacaa caacaattac gggtattgaa atgttccaaa aaactttaga ggaaggtttt
    20881 gccggtgata aaaaacttta gaggaaggtt ttgccggtga taatgaagag cctggttgcc
    20941 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
    21001 ccgtgtgcga gacaccgcgg ccggccgccg gcgttgtgga tacctcgcgg aaaacttggc
    21061 cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc
    21121 gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca
    21181 aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg
    21241 gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg
    21301 atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga
    21361 ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca
    21421 ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca
    21481 gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac
    21541 cctcccggtc gagtgagcga ggaagcacca gggaacagca cttatatatt ctgcttacac
    21601 acgatgcctg aaaaaacttc ccttggggtt atccacttat ccacggggat atttttataa
    21661 ttattttttt tatagttttt agatcttctt ttttagagcg ccttgtaggc ctttatccat
    21721 gctggttcta gagaaggtgt tgtgacaaat tgccctttaa gtgtgacaaa tcaccctcaa
    21781 atgacagtca tgtctgtgac aaattgccct taaccctgtg acaaattgcc ctcagaagaa
    21841 gctgtttttt cacaaagtta tccctgctta ttgactcttt tttatttagt gtgacaatct
    21901 aaaaacttgt cacacttcac atggatctgt c
//
