LOCUS       sequence-420239-        9807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, 
            W102L, W166L mutations installed into EIF4E).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pPB_EF1a_Gag-EIF4E-TM
FEATURES             Location/Qualifiers
     source          1..9807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   127..189
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1357..2534
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1587..2525
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2482..2502
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2541..2550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2550..2966
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2896..2918
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2934..2950
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4827..4883
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      5133..5142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5630..5751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5667..5686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5721..5740
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6514..6548
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7039..7055)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7039..7055)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7052..7074)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7063..7079
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7087..7093,7094..7111,7112..7117))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7132..7153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7270..7287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7441..8029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7521..7540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8200..8991,8992..9060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8823..8842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9061..9165)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9191..9646)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9328..9349)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9773..9795
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9787..9804
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9788..9804
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat
       61 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagcta
      121 gattaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgtgtaaaat
      181 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
      241 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
      301 tttatttatt tattaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt
      361 ttatgaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
      421 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
      481 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
      541 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaggcc
      601 tccaccataa agatggttaa ttaacccacc caagatctgg atcgataagc ttgatatcga
      661 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
      721 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
      781 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
      841 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
      901 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
      961 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     1021 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     1081 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     1141 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg ccccgcgcgc
     1201 agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga gtcctgcagc
     1261 cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     1321 ctgtaggttt ggcaatcttg aaaggagtgg gaattagctc cggtgcccgt cagtgggcag
     1381 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
     1441 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     1501 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     1561 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     1621 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     1681 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     1741 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     1801 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     1861 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     1921 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     1981 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     2041 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     2101 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     2161 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     2221 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     2281 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     2341 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     2401 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     2461 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     2521 ttcaggtgtc gtgacgtacg gccaccatgg gccagactgt taccactccc ttaagtttga
     2581 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     2641 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     2701 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     2761 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     2821 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     2881 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     2941 cagccctcac tccttctcta ggcgccaaac ctaaacctca agttctttct gacagtgggg
     3001 ggccgctcat cgacctactt acagaagacc ccccgcctta tagggaccca agaccacccc
     3061 cttccgacag ggacggaaat ggtggagaag cgacccctgc gggagaggca ccggacccct
     3121 ccccaatggc atctcgccta cgtgggagac gggagccccc tgtggccgac tccactacct
     3181 cgcaggcatt ccccctccgc gcaggaggaa acggacagct tcaatactgg ccgttctcct
     3241 cttctgacct ttacaactgg aaaaataata acccttcttt ttctgaagat ccaggtaaac
     3301 tgacagctct gatcgagtct gtcctcatca cccatcagcc cacctgggac gactgtcagc
     3361 agctgttggg gactctgctg accggagaag aaaaacaacg ggtgctctta gaggctagaa
     3421 aggcggtgcg gggcgatgat gggcgcccca ctcaactgcc caatgaagtc gatgccgctt
     3481 ttcccctcga gcgcccagac tgggattaca ccacccaggc aggtaggaac cacctagtcc
     3541 actatcgcca gttgctccta gcgggtctcc aaaacgcggg cagaagcccc accaatttgg
     3601 ccaaggtaaa aggaataaca caagggccca atgagtctcc ctcggccttc ctagagagac
     3661 ttaaggaagc ctatcgcagg tacactcctt atgaccctga ggacccaggg caagaaacta
     3721 atgtgtctat gtctttcatt tggcagtctg ccccagacat tgggagaaag ttagagaggt
     3781 tagaagattt aaaaaacaag acgcttggag atttggttag agaggcagaa aagatcttta
     3841 ataaacgaga aaccccggaa gaaagagagg aacgtatcag gagagaaaca gaggaaaaag
     3901 aagaacgccg taggacagag gatgagcaga aagagaaaga aagagatcgt aggagacata
     3961 gagagatgag caagctattg gccactgtcg ttagtggaca gaaacaggat agacagggag
     4021 gagaacgaag gaggtcccaa ctcgatcgcg accagtgtgc ctactgcaaa gaaaaggggc
     4081 actgggctaa agattgtccc aagaaaccac gaggacctcg gggaccaaga ccgcagggat
     4141 caggatcagg atcaggatca ggatctatgg cgactgtcga accggaaacc acccctactc
     4201 ctaatccccc gactacagaa gaggagaaaa cggaatctaa tcaggaggtt gctaacccag
     4261 aacactatat taaacatccc ctacagaaca gatgggcact ctggtttttt aaaaatgata
     4321 aaagcaaaac tttgcaagca aacctgcggc tgatctccaa gtttgatact gttgaagact
     4381 tttgggctct gtacaaccat atccagttgt ctagtaattt aatgcctggc tgtgactact
     4441 cactttttaa ggatggtatt gagcctatgt tggaagatga gaaaaacaaa cggggaggac
     4501 gatggctaat tacattgaac aaacagcaga gacgaagtga cctcgatcgc ttttggctag
     4561 agacacttct gtgccttatt ggagaatctt ttgatgacta cagtgatgat gtatgtggcg
     4621 ctgttgttaa tgttagagct aaaggtgata agatagcaat attgactact gaatgtgaaa
     4681 acagagaagc tgttacacat atagggaggg tatacaagga aaggttagga cttcctccaa
     4741 agatagtgat tggttatcag tcccacgcag acacagctac taagagcggc tccaccacta
     4801 aaaataggtt tgttgttgga tctggcgcaa caaacttctc tctgctgaaa caagccggag
     4861 atgtcgaaga gaatcctgga ccggtcagca agggcgagga ggacaacgcc tctctcccag
     4921 cgacacacga gttacacatc tttggctcca tcaacggtgt ggacttcgac ctcgtgggtc
     4981 agggcaccgg caatccaaac gacggttacg aggagctcaa cctcaagtcc accaagggcg
     5041 acctccagtt ctccccctgg attctggtcc ctcatatcgg gtacggcttc catcagtacc
     5101 tgccctaccc agacgggatg tcgcctttcc aggccgccat ggtggacggc tccggatacc
     5161 aagtccatcg cacaatgcag ttcgaagacg gtgcctccct tactgtcaac taccgctaca
     5221 cctacgaggg aagccacatc aaaggagagg cccaggtcaa ggggactggt ttccctgccg
     5281 acggtcctgt cctcaccaac tcgctcaccg ctgcggactg gtgcaggtcg aagaagactt
     5341 accccaacga caaaaccatc atcagtacct tcaagtggag ttacaccact ggaaacggca
     5401 agcgctaccg gagcactgcg cggaccacct acacctttgc caagccaatg gcggccaact
     5461 atctcaagaa ccagccgatg tacgtgttcc gcaagacgga gctcaagcac tccaagaccg
     5521 agctcaactt caaggagtgg caaaaggcct ttaccgacgt catgggcatg gacgagctct
     5581 acaagtaaca atcaacctct ggattacaaa atttgtggtt aactaactaa acttgtttat
     5641 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5701 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5761 gaattgactc aaatgatgtc aattagtcta tcagaagctc atctggtctc ccttccgggg
     5821 gacaagacat ccctgtttaa tatttaaaca gcagtgttcc caaactgggt tcttatatcc
     5881 cttgctctgg tcaaccaggt tgcagggttt cctgtcctca caggaacgaa gtccctaaag
     5941 aaacagtggc agccaggttt agccccggaa ttgactggat tcctttttta gggcccattg
     6001 gtatggcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
     6061 ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc
     6121 ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc
     6181 cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctg
     6241 atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
     6301 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
     6361 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
     6421 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
     6481 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
     6541 ttctagggtt aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc
     6601 atcgcccaag ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg
     6661 aggttgaagc ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc
     6721 gaaaacgcac gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg
     6781 aactgttcgt tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc
     6841 ggatggtcag cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc
     6901 gtgccggtgt gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg
     6961 ggtatcctgg ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc
     7021 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7081 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7141 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7201 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7261 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7861 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8161 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9181 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     9241 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     9301 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     9361 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     9421 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     9481 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     9541 gggaagaaag cgacaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     9601 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     9661 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     9721 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     9781 cgacgttgta aaacgacggc cagtgag
//
