LOCUS       sequence-420243-       10407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac vector to express HA-VSVG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pPB_EF1a_HA-VSVG
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   127..189
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1356..2534
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1587..2525
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2482..2502
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2625..2651
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4098..4130
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             4146..4202
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4920..4976
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4977..5576
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4977..4996)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5473..5493
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5592..6180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5645..5665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6063..6074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6230..6351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6267..6286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6321..6340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7114..7148
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7639..7655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7639..7655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7652..7674)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7663..7679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7687..7693,7694..7711,7712..7717))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7732..7753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7870..7887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8041..8629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8121..8140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8800..9591,9592..9660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9423..9442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9661..9765)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9791..10246)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9928..9949)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10140..10159
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10373..10395
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10387..10404
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10388..10404
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat
       61 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagcta
      121 gattaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgtgtaaaat
      181 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
      241 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
      301 tttatttatt tattaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt
      361 ttatgaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
      421 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
      481 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
      541 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaggcc
      601 tccaccataa agatggttaa ttaacccacc caagatctgg atcgataagc ttgatatcga
      661 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
      721 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
      781 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
      841 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
      901 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
      961 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     1021 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     1081 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     1141 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg ccccgcgcgc
     1201 agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga gtcctgcagc
     1261 cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     1321 ctgtaggttt ggcaatcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     1381 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
     1441 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     1501 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     1561 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     1621 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     1681 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     1741 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     1801 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     1861 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     1921 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     1981 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     2041 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     2101 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     2161 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     2221 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     2281 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     2341 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     2401 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     2461 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     2521 ttcaggtgtc gtgacgtacg gccaccatga agtgcctttt gtacttagcc tttttattca
     2581 ttggggtgaa ttgcaagttc accatagttt ttccatccgg aggataccca tacgatgttc
     2641 cagattacgc tggcggaagc ttgggacaca accaaaaagg aaactggaaa aatgttcctt
     2701 ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca
     2761 cagccttaca agtcaaaatg cccaagagtc acaaggctat tcaagcagat ggttggatgt
     2821 gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa
     2881 cacattccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa
     2941 cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg
     3001 tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat
     3061 acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc
     3121 ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt
     3181 ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg
     3241 gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct
     3301 gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga
     3361 tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta
     3421 tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct
     3481 tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc
     3541 cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa
     3601 tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa
     3661 tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg
     3721 actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag
     3781 gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta
     3841 gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg
     3901 atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag
     3961 aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa
     4021 tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca
     4081 ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag gaattcggat
     4141 ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac
     4201 cgatggtgtc caagggcgag gaagataaca tggccatcat caaggagttc atgaggttta
     4261 aggtccacat ggagggttca gtcaatggcc acgagttcga gattgaaggc gagggcgagg
     4321 gccgccccta cgaagggaca cagacggcga aattgaaggt gaccaaaggc gggccattgc
     4381 ccttcgcatg ggacatcttg tcccctcagt ttatgtatgg cagcaaggcc tatgttaagc
     4441 accccgctga tatcccggac tacttgaagc tgtcctttcc agaggggttt aaatgggagc
     4501 gcgttatgaa tttcgaagac ggaggagtgg ttacggtgac gcaggactca tccctgcagg
     4561 acggagaatt tatatataag gttaagttga gaggcacaaa cttcccaagc gacggccctg
     4621 tgatgcagaa gaaaacaatg gggtgggaag cttccagcga gcgcatgtac cccgaagatg
     4681 gcgccctcaa gggcgagata aagcaaaggc tgaaacttaa ggacggcggt cattacgacg
     4741 cggaggtcaa gacaacttac aaggctaaaa aacccgttca gttgcctggg gcttacaatg
     4801 ttaatatcaa acttgacatc acaagccaca atgaagacta tacgatcgtg gagcagtatg
     4861 aacgagcgga aggcaggcac tcaacggggg ggatggacga gctttacaag ggatccggcg
     4921 caacaaactt ctctctgctc aaacaagccg gcgacgtcga agagaatcct gggccgatga
     4981 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     5041 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     5101 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     5161 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     5221 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     5281 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     5341 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     5401 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5461 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     5521 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctaaacgc
     5581 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5641 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5701 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5761 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5821 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5881 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5941 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6001 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6061 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6121 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6181 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct aactaactaa acttgtttat
     6241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6361 gaattgactc aaatgatgtc aattagtcta tcagaagctc atctggtctc ccttccgggg
     6421 gacaagacat ccctgtttaa tatttaaaca gcagtgttcc caaactgggt tcttatatcc
     6481 cttgctctgg tcaaccaggt tgcagggttt cctgtcctca caggaacgaa gtccctaaag
     6541 aaacagtggc agccaggttt agccccggaa ttgactggat tcctttttta gggcccattg
     6601 gtatggcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
     6661 ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc
     6721 ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc
     6781 cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctg
     6841 atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
     6901 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
     6961 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
     7021 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
     7081 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
     7141 ttctagggtt aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc
     7201 atcgcccaag ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg
     7261 aggttgaagc ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc
     7321 gaaaacgcac gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg
     7381 aactgttcgt tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc
     7441 ggatggtcag cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc
     7501 gtgccggtgt gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg
     7561 ggtatcctgg ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc
     7621 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7681 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7741 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7801 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7861 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7921 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8461 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8701 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8761 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8821 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8881 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8941 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9001 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9061 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9781 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     9841 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     9901 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     9961 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
    10021 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
    10081 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
    10141 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
    10201 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
    10261 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    10321 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10381 cgacgttgta aaacgacggc cagtgag
//
