LOCUS       sequence-42025-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength phosphonate sensor constructed from E. coli PhnD
            and cpGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Alicea I, Marvin JS, Miklos AE, Ellington AD, Looger LL, Schreiter 
            ER
  TITLE     Structure of the Escherichia coli Phosphonate Binding Protein PhnD 
            and Rationally Optimized Phosphonate Biosensors.
  JOURNAL   J Mol Biol. 2011 Oct 12.
  PUBMED    22019591
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Oct 12."
COMMENT     Sequence Label: EcPhnD90.L1∆∆AD.L297RL301R.pRSET
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     539..560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(666..687)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgca tcatcaccac caccatggat
       61 ccgaagaaca agaaaaagct ctgaacttcg gtatcatctc cactgaaagc caacagaatc
      121 tgaagccgca gtggaccccg ttcctgcagg atatggagaa gaaactgggc gttaaagtca
      181 acgctttctt cgctccggac tacgcaggca tcatccaggg tatgcgtttc aacaaagtcg
      241 atatcgcctg gtacggcaat ctgtctgcaa tggaagcggt cgatcgtgcc aacggtcagg
      301 tgttcgcgca gacggttgca gcggacgcgg acaacgtcta tatcatggcc gacaagcaga
      361 agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
      421 tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      481 accactacct gagcacccag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca
      541 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
      601 agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      661 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      721 gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      781 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagc
//
