LOCUS       sequence-42027-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength phosphonate sensor constructed from E. coli PhnD
            and cpGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Alicea I, Marvin JS, Miklos AE, Ellington AD, Looger LL, Schreiter 
            ER
  TITLE     Structure of the Escherichia coli Phosphonate Binding Protein PhnD 
            and Rationally Optimized Phosphonate Biosensors.
  JOURNAL   J Mol Biol. 2011 Oct 12.
  PUBMED    22019591
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Oct 12."
COMMENT     Sequence Label: EcPhnD90.L1∆∆AD.L297RL301R.pRSET
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..103)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(586..603)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             614..636
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(667..686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(771..788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctctcgccc ttgctcacca tgctccctcc ggtaccgccc ttgtacagct cgtccatgcc
       61 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      121 ggggtctttc gaaagtttgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
      181 gccgtcgccg atgggggtgt tctgctggta gtggtaggcg agctgcacgc cgccgtcctc
      241 gatgttgtgg cggatcttga agttcgcctt gatgccgttc ttctgcttgt cggccatgat
      301 atagacgttg tccgcgtccg ctgcaaccgt ctgcgcgaac acctgaccgt tggcacgatc
      361 gaccgcttcc attgcagaca gattgccgta ccaggcgata tcgactttgt tgaaacgcat
      421 accctggatg atgcctgcgt agtccggagc gaagaaagcg ttgactttaa cgcccagttt
      481 cttctccata tcctgcagga acggggtcca ctgcggcttc agattctgtt ggctttcagt
      541 ggagatgata ccgaagttca gagctttttc ttgttcttcg gatccatggt ggtggtgatg
      601 atgcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg
      661 tggtctccct atagtgagtc gtattaattt cgcgggatcg agatcctgca ttaatgaatc
      721 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc a
//
