LOCUS       sequence-420274-       10480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MS2 hairpin-containing expression vector and sgRNA entry plasmid for
            the directed export of barcode transcripts in Gag-MCP virus-like 
            particles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: CROPseq-Puro-4xMS2
FEATURES             Location/Qualifiers
     source          1..10480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        288..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1778..1822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2003..2120
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2259..3437
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2490..3428
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3385..3405
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3450..4049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3450..3469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3946..3966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_RNA        4106..4124
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4145..4163
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4215..4233
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4254..4272
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_feature    4321..4909
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4374..4394)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4792..4803)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5065..5313
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5065..5085
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5236..5255
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             7333..7513
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7591..7712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7628..7647)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7682..7701
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7752..7887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7907..7926)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7908..7926)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7936..7953)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7936..7952)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7945..7967)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8094..8549
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8181..8200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8391..8412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8575..8679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8680..8748,8749..9540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8898..8917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9711..10299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10200..10219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10453..10470
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgac
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga atcttgagac
     1981 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2041 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2101 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     2161 ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca
     2221 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2281 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
     2341 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2401 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2461 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2521 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2581 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2641 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2701 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2761 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2821 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2881 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2941 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     3001 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     3061 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3121 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3181 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     3241 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3301 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3361 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3421 catttcaggt gtcgtgacgt acggccacca tgaccgagta caagcccacg gtgcgcctcg
     3481 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3541 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     3601 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     3661 ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     3721 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     3781 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct
     3841 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     3901 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     3961 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4021 ggtgcatgac ccgcaagccc ggtgcctgat ggacgagctg tacaagtaaa gcggccggat
     4081 cctaaggtac ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcta
     4141 gaaaacatga ggatcaccca tgtctgcagt attcccgggt tcattagatc ctaaggtacc
     4201 taattgccta gaaaacatga ggatcaccca tgtctgcagg tcgactccag aaaacatgag
     4261 gatcacccat gtctgcagta ttcccgggtt cattagatct gcgcgcgatc gatagatcct
     4321 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4381 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4441 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4501 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4561 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4621 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4681 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4741 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4801 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4861 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     4921 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4981 tggaagggct aattcactcc caacgaagac aagatttcac cattatcgtt tcagacccac
     5041 ctcccaaccc cgaggggacc cagagagggc ctatttccca tgattccttc atatttgcat
     5101 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
     5161 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     5221 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     5281 gctttatata tcttgtggaa aggacgaaac accggagacg gttgtaaatg agcacacaaa
     5341 atacacatgc taaaatatta tattctatga cctttataaa atcaaccaaa atcttctttt
     5401 taataacttt agtatcaata attagaattt ttatgttcct ttttgcaaac ttttaataaa
     5461 aatgagcaaa ataaaaaaac gctagtttta gtaactcgcg ttgttttctt cacctttaat
     5521 aatagctact ccaccacttg ttcctaagcg gtcagctcct gcttcaatca ttttttgagc
     5581 atcttcaaat gttctaactc caccagctgc tttaactaaa gcattgtctt taacaactga
     5641 cttcattagt ttaacatctt caaatgttgc acctgatttt gaaaatcctg ttgatgtttt
     5701 aacaaattct aatccagctt caacagctat ttcacaagct ttcatgattt cttcttttgt
     5761 taataaacaa ttttccataa tacatttaac aacatgtgat ccagctgctt tttttacagc
     5821 tttcatgtct tctaaaacta attcataatt tttgtctttt aatgcaccaa tatttaatac
     5881 catatcaatt tctgttgcac catctttaat tgcttcagaa acttcgaatg cttttgtagc
     5941 tgttgtgcat gcacctagag gaaaacctac aacatttgtt attcctacat ttgtgccttt
     6001 taataattct ttacaatagc ttgttcaata tgaattaaca caaactgttg caaaatcaaa
     6061 ttcaattgct tcatcacata attgtttaat ttcagctttc gtagcatctt gttttaataa
     6121 tgtgtgatct atatatttgt ttagtttcat tttttctcct atatattcat ttttaatttt
     6181 aattctttaa taatttcgtc tactttaact ttagcgtttt gaacagattc accaacacct
     6241 ataaaataaa tttttagttt aggttcagtt ccacttgggc gaacagcaaa tcatgactta
     6301 tcttctaaat aaaattttag taagtcttgt cctggcatat tatacattcc atcgatgtag
     6361 tcttcaacat taacaacttt aagtccagca atttgagtta agggtgttgc tctcaatgat
     6421 ttcattaatg gttcaatttt taatttcttt tcttctggtt taaaattcaa gtttaaagtg
     6481 aaagtgtaat atgcacccat ttctttaaat aaatcttcta aatagtctac taatgtttta
     6541 ttttgttttt tataaaatca agcagcctct gctattaata tagaagcttg tattccatct
     6601 ttatctctag ctgagtcatc aattacatat ccataacttt cttcataagc aaaaacaaaa
     6661 tttaatccgt tatcttcttc tttagcaatt tctctaccca ttcatttaaa tccagttaaa
     6721 gtttttacaa tattaactcc atatttttca tgagcgattc tatcacccaa atcacttgtt
     6781 acaaaacttg aatatagagc cggatttttt ggaatgctat ttaagcgttt tagatttgat
     6841 aattttcaat caattaaaat tggtcctgtt tgatttccat ctaatcttac aaaatgacca
     6901 tcatgtttta ttgccattcc aaatctgtca gcatctgggt cattcataat aataatatct
     6961 gcatcatgtt taataccata ttcaagcggt atttttcatg caggatcaaa ttctggattt
     7021 ggatttacaa catttttaaa tgtttcatct tcaaatgcat gctcttcaac ctcaataacg
     7081 ttatatcctg attcacgtaa tatttttggg gtaaatttag ttcctgttcc attaactgcg
     7141 ctaaaaataa tttttaaatc ttttttagct tcttgctctt ttttgtacgt ctctgtttca
     7201 gagctatgct ggaaacagca tagcaagttg aaataaggct agtccgttat caacttgaaa
     7261 aagtggcacc gagtcggtgc ttttttaagc ttggcgtaac tagatcttga gacactgctt
     7321 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7381 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7441 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7501 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     7561 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7621 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7681 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7741 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7801 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7861 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     7921 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7981 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8041 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     8101 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8161 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8221 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8281 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8341 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8401 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8461 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8521 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8581 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8641 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8701 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8761 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8821 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8881 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8941 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9001 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9061 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9121 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9181 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9241 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9301 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9361 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9421 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9481 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9541 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9601 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9661 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9721 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9781 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9841 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     9901 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9961 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10021 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10081 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10141 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10201 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10261 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10321 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    10381 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    10441 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
