LOCUS       sequence-420278-       10371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MS2 hairpin-containing expression vector and sgRNA entry plasmid for
            the directed export of barcode transcripts in Gag-MCP virus-like 
            particles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: CROPseq-Puro-2xMS2
FEATURES             Location/Qualifiers
     source          1..10371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        288..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1778..1822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2003..2120
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2259..3437
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2490..3428
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3385..3405
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3450..4049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3450..3469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3946..3966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_RNA        4106..4124
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4145..4163
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_feature    4212..4800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4265..4285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4683..4694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4956..5204
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4956..4976
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5127..5146
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             7224..7404
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7482..7603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7519..7538)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7573..7592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7643..7778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7798..7817)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7799..7817)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7827..7844)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7827..7843)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7836..7858)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7985..8440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8072..8091)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8282..8303
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8466..8570
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8571..8639,8640..9431)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8789..8808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9602..10190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10091..10110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10344..10361
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgac
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga atcttgagac
     1981 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2041 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2101 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     2161 ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca
     2221 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2281 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
     2341 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2401 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2461 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2521 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2581 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2641 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2701 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2761 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2821 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2881 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2941 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     3001 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     3061 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3121 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3181 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     3241 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3301 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3361 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3421 catttcaggt gtcgtgacgt acggccacca tgaccgagta caagcccacg gtgcgcctcg
     3481 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3541 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     3601 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     3661 ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     3721 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     3781 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct
     3841 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     3901 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     3961 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4021 ggtgcatgac ccgcaagccc ggtgcctgat ggacgagctg tacaagtaaa gcggccggat
     4081 cctaaggtac ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcta
     4141 gaaaacatga ggatcaccca tgtctgcagt attcccgggt tcattagatc tgcgcgcgat
     4201 cgatagatcc taatcaaccc ctggattaca aaatttgtga aagattgact ggtattctta
     4261 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4321 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4381 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4441 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4501 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4561 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     4621 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4681 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4741 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4801 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4861 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatttca ccattatcgt
     4921 ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc atgattcctt
     4981 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     5041 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     5101 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     5161 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggagac ggttgtaaat
     5221 gagcacacaa aatacacatg ctaaaatatt atattctatg acctttataa aatcaaccaa
     5281 aatcttcttt ttaataactt tagtatcaat aattagaatt tttatgttcc tttttgcaaa
     5341 cttttaataa aaatgagcaa aataaaaaaa cgctagtttt agtaactcgc gttgttttct
     5401 tcacctttaa taatagctac tccaccactt gttcctaagc ggtcagctcc tgcttcaatc
     5461 attttttgag catcttcaaa tgttctaact ccaccagctg ctttaactaa agcattgtct
     5521 ttaacaactg acttcattag tttaacatct tcaaatgttg cacctgattt tgaaaatcct
     5581 gttgatgttt taacaaattc taatccagct tcaacagcta tttcacaagc tttcatgatt
     5641 tcttcttttg ttaataaaca attttccata atacatttaa caacatgtga tccagctgct
     5701 ttttttacag ctttcatgtc ttctaaaact aattcataat ttttgtcttt taatgcacca
     5761 atatttaata ccatatcaat ttctgttgca ccatctttaa ttgcttcaga aacttcgaat
     5821 gcttttgtag ctgttgtgca tgcacctaga ggaaaaccta caacatttgt tattcctaca
     5881 tttgtgcctt ttaataattc tttacaatag cttgttcaat atgaattaac acaaactgtt
     5941 gcaaaatcaa attcaattgc ttcatcacat aattgtttaa tttcagcttt cgtagcatct
     6001 tgttttaata atgtgtgatc tatatatttg tttagtttca ttttttctcc tatatattca
     6061 tttttaattt taattcttta ataatttcgt ctactttaac tttagcgttt tgaacagatt
     6121 caccaacacc tataaaataa atttttagtt taggttcagt tccacttggg cgaacagcaa
     6181 atcatgactt atcttctaaa taaaatttta gtaagtcttg tcctggcata ttatacattc
     6241 catcgatgta gtcttcaaca ttaacaactt taagtccagc aatttgagtt aagggtgttg
     6301 ctctcaatga tttcattaat ggttcaattt ttaatttctt ttcttctggt ttaaaattca
     6361 agtttaaagt gaaagtgtaa tatgcaccca tttctttaaa taaatcttct aaatagtcta
     6421 ctaatgtttt attttgtttt ttataaaatc aagcagcctc tgctattaat atagaagctt
     6481 gtattccatc tttatctcta gctgagtcat caattacata tccataactt tcttcataag
     6541 caaaaacaaa atttaatccg ttatcttctt ctttagcaat ttctctaccc attcatttaa
     6601 atccagttaa agtttttaca atattaactc catatttttc atgagcgatt ctatcaccca
     6661 aatcacttgt tacaaaactt gaatatagag ccggattttt tggaatgcta tttaagcgtt
     6721 ttagatttga taattttcaa tcaattaaaa ttggtcctgt ttgatttcca tctaatctta
     6781 caaaatgacc atcatgtttt attgccattc caaatctgtc agcatctggg tcattcataa
     6841 taataatatc tgcatcatgt ttaataccat attcaagcgg tatttttcat gcaggatcaa
     6901 attctggatt tggatttaca acatttttaa atgtttcatc ttcaaatgca tgctcttcaa
     6961 cctcaataac gttatatcct gattcacgta atatttttgg ggtaaattta gttcctgttc
     7021 cattaactgc gctaaaaata atttttaaat cttttttagc ttcttgctct tttttgtacg
     7081 tctctgtttc agagctatgc tggaaacagc atagcaagtt gaaataaggc tagtccgtta
     7141 tcaacttgaa aaagtggcac cgagtcggtg cttttttaag cttggcgtaa ctagatcttg
     7201 agacactgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7261 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7321 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7381 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     7441 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7501 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7561 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7621 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7681 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7741 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7801 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7861 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7921 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7981 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8041 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8101 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8161 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8221 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8281 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8341 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8401 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8461 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8521 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8581 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8641 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8701 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8761 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8821 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8881 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8941 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9001 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9061 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9121 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9181 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9241 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9301 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9361 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9421 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9481 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9541 cttaacgtga gttttcgttc cactgagcgc cagaccccgt agaaaagatc aaaggatctt
     9601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9721 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9961 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10261 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10321 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
