LOCUS       sequence-420313-       10216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell 
            surface via a GPI anchor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q,
            Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C
  TITLE     GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 
            mediating Zika virus entry.
  JOURNAL   iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481.
            eCollection 2022 Dec 22.
  PUBMED    36404916
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-2"; volume: "25(12):105481. doi"; pages: " 
            10.1016/j.isci.2022.105481. eCollection 2022 Dec 22"
COMMENT     Sequence Label: pHAGE-PrP (1-22)-RBP(V)-13 x
            Linker-BioID2-HA-...
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4466..5161
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             5162..5188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5315..5883
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5333..5885
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5477..5494)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5704..5723
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5897..6592
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    6606..7194
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6659..6679)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7077..7088)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7269..7502
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7621..7639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7707..7811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7812..7880,7881..8672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8030..8049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8843..9431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9332..9351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9585..9602
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9842..9863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9878..9883,9884..9901,9902..9908)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9916..9932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9921..9943
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9940..9956
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9940..9956
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10006..10025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccgc accggtctgc
     2821 agctagcatg gcgaaccttg gctgctggat gctggttctc tttgtggcca catggagtga
     2881 cctgggcctc tgcaagttca ccatagtttt tccacacaac caaaaaggaa actggaaaaa
     2941 tgttccttct aattaccatt attgcccgtc aagctcagat ttaaattggc ataatgactt
     3001 aataggcaca gccttacaag tcaaaatgcc caagagtcac aaggctattc aagcagacgg
     3061 ttggatgtgt catgcttcca aatgggtcac tacttgtgat ttccgctggt atggaccgaa
     3121 gtatataaca cagtccatcc gatccttcac tccatctgta gaacaatgca aggaaagcat
     3181 tgaacaaacg aaacaaggaa cttggctgaa tccaggcttc cctcctcaaa gttgtggata
     3241 tgcaactgtg acggatgccg aagcagtgat tgtccaggtg actcctcacc atgtgctggt
     3301 tgatgaatac acaggagaat gggttgattc acagttcatc aacggaaaat gcagcaatta
     3361 catatgcccc actgtccata actctacaac ctggcattct gactataagg tcaaagggct
     3421 atgtgattct aacctcattt ccatggacat caccttcttc tcagaggacg gagagctatc
     3481 atccctggga aaggagggca cagggttcag aagtaactac tttgcttatg aaactggagg
     3541 caaggcctgc aaaatgcaat actgcaagca ttggggagtc agactcccat caggtgtctg
     3601 gttcgagatg gctgataagg atctctttgc tgcagccaga ttccctgaat gcccagaagg
     3661 gtcaagtatc tctgctccat ctcagacctc agtggatgta agtctaattc aggacgttga
     3721 gaggatcttg gattattccc tctgccaaga aacctggagc aaaatcagag cgggtcttcc
     3781 aatctctcca gtggatctca gctatcttgc tcctaaaaac ccaggaaccg gtcctgcttt
     3841 caccataatc aatggtaccc taaaatactt tgagaccaga tacatcagag tcgatattgc
     3901 tgctccaatc ctctcaagaa tggtcggaat gatcagtgga actaccacag aaagggaact
     3961 gtgggatgac tgggcaccat atgaagacgt ggaaattgga cccaatggag ttctgaggac
     4021 cagttcagga tataagtttc ctttatacat gattggacat ggtatgttgg actccgatct
     4081 tcatcttagc tcaaaggctc aggtgttcga acatcctcac attcaagacg ctgcttcgca
     4141 acttcctgat gatgagagtt tattttttgg tgatactggg ctatccaaaa atccaatcga
     4201 gcttgtagaa ggttggttca gtagttggaa aagctctatt gcctctgaat tcggatccgg
     4261 tggaggcggg tctggaggcg ggggtagtgg cgggggtgga agcgggggtg gaggcgggtc
     4321 gggtggcgga ggtagcggag gcggtggaag tggaggcggt ggaagtggtg gaggaggttc
     4381 tggtggcgga ggtagcggtg gcggaggtag cggaggcggt ggaagtggtg gcggaggtag
     4441 cggaggcggt ggatcggcgc gccgtttcaa gaacctgatc tggctgaagg aggtggacag
     4501 cacccaggag agactgaagg agtggaacgt tagctacggc accgccctgg tggccgacag
     4561 acagaccaag ggcagaggcg gcctgggcag aaagtggctg agccaggagg gcggcctgta
     4621 cttcagcttc ctgctgaacc ccaaggagtt cgagaacctg ctgcagctgc ccctggtgct
     4681 gggcctgagc gtgagcgagg ccctggagga gatcaccgag atccccttca gcctgaagtg
     4741 gcccaacgac gtgtacttcc aggagaagaa ggtgagcggc gtgctgtgcg agctgagcaa
     4801 ggacaagctg atcgtgggca tcggcatcaa cgtgaaccag agagagatcc ccgaggagat
     4861 caaggacaga gccaccaccc tgtacgagat caccggcaag gactgggaca gaaaggaggt
     4921 gctgctgaag gtgctgaaga gaatcagcga gaacctgaag aagttcaagg agaagagctt
     4981 caaggagttc aagggcaaga tcgagagcaa gatgctgtac ctgggcgagg aggtgaagct
     5041 gctgggcgag ggcaagatca ccggcaagct ggtgggcctg agcgagaagg gcggcgccct
     5101 gatcctgacc gaggagggca tcaaggagat cctgagcggc gagttcagcc tgagaagaag
     5161 ctacccttac gatgtaccgg attacgcatc tagaggaagt ggaaccactt caggtactac
     5221 ccgtcttcta tctgggcaca cgtgtttcac gttgacaggt ttgcttggga cgctagtaac
     5281 catgggcttg ctgacttagc tcgagtctag aggatccctc cccccccccc taacgttact
     5341 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     5401 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     5461 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     5521 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     5581 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     5641 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     5701 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     5761 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     5821 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     5881 taatatggcc acacatatgg cccagtccaa gcacggcctg accaaggaga tgaccatgaa
     5941 gtaccgcatg gagggctgcg tggacggcca caagttcgtg atcaccggcg agggcatcgg
     6001 ctaccccttc aagggcaagc aggccatcaa cctgtgcgtg gtggagggcg gccccttgcc
     6061 cttcgccgag gacatcttgt ccgccgcctt catgtacggc aaccgcgtgt tcaccgagta
     6121 cccccaggac atcgtcgact acttcaagaa ctcctgcccc gccggctaca cctgggaccg
     6181 ctccttcctg ttcgaggacg gcgccgtgtg catctgcaac gccgacatca ccgtgagcgt
     6241 ggaggagaac tgcatgtacc acgagtccaa gttctacggc gtgaacttcc ccgccgacgg
     6301 ccccgtgatg aagaagatga ccgacaactg ggagccctcc tgcgagaaga tcatccccgt
     6361 gcccaagcag ggcatcttga agggcgacgt gagcatgtac ctgctgctga aggacggtgg
     6421 ccgcttgcgc tgccagttcg acaccgtgta caaggccaag tccgtgcccc gcaagatgcc
     6481 cgactggcac ttcatccagc acaagctgac ccgcgaggac cgcagcgacg ccaagaacca
     6541 gaagtggcac ctgaccgagc acgccatcgc ctccggctcc gccttgccct gaatcgatag
     6601 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6661 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6721 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6781 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6841 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6901 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6961 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7021 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7081 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7141 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga
     7201 tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7261 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7321 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7381 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7441 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7501 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7561 agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg
     7621 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7681 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7741 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7801 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7861 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7921 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7981 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8041 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8101 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8161 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8221 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8281 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8341 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8401 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8461 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8521 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8581 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8641 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8701 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8761 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8821 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8881 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8941 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9001 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9061 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9121 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9181 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9241 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9301 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9361 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9421 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9481 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9541 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9601 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9661 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9721 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9781 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9841 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9901 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
     9961 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10021 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    10081 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    10141 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    10201 ctacaaactt agtagt
//
