LOCUS       sequence-420314-       10222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell 
            surface via a GPI anchor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q,
            Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C
  TITLE     GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 
            mediating Zika virus entry.
  JOURNAL   iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481.
            eCollection 2022 Dec 22.
  PUBMED    36404916
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-2"; volume: "25(12):105481. doi"; pages: " 
            10.1016/j.isci.2022.105481. eCollection 2022 Dec 22"
COMMENT     Sequence Label: pHAGE-PrP (1-22)-BioID2-HA-13 x Linker-
            RBP(V)...
FEATURES             Location/Qualifiers
     source          1..10222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2894..3589
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             3590..3616
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5321..5889
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5339..5891
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5483..5500)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5710..5729
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5903..6598
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    6612..7200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6665..6685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7083..7094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7275..7508
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7627..7645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7713..7817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7818..7886,7887..8678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8036..8055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8849..9437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9338..9357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9591..9608
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9848..9869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9884..9889,9890..9907,9908..9914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9922..9938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9927..9949
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9946..9962
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9946..9962
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10012..10031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccgc accggtctgc
     2821 agctagcatg gcgaaccttg gctgctggat gctggttctc tttgtggcca catggagtga
     2881 cctgggcctc tgcttcaaga acctgatctg gctgaaggag gtggacagca cccaggagag
     2941 actgaaggag tggaacgtta gctacggcac cgccctggtg gccgacagac agaccaaggg
     3001 cagaggcggc ctgggcagaa agtggctgag ccaggagggc ggcctgtact tcagcttcct
     3061 gctgaacccc aaggagttcg agaacctgct gcagctgccc ctggtgctgg gcctgagcgt
     3121 gagcgaggcc ctggaggaga tcaccgagat ccccttcagc ctgaagtggc ccaacgacgt
     3181 gtacttccag gagaagaagg tgagcggcgt gctgtgcgag ctgagcaagg acaagctgat
     3241 cgtgggcatc ggcatcaacg tgaaccagag agagatcccc gaggagatca aggacagagc
     3301 caccaccctg tacgagatca ccggcaagga ctgggacaga aaggaggtgc tgctgaaggt
     3361 gctgaagaga atcagcgaga acctgaagaa gttcaaggag aagagcttca aggagttcaa
     3421 gggcaagatc gagagcaaga tgctgtacct gggcgaggag gtgaagctgc tgggcgaggg
     3481 caagatcacc ggcaagctgg tgggcctgag cgagaagggc ggcgccctga tcctgaccga
     3541 ggagggcatc aaggagatcc tgagcggcga gttcagcctg agaagaagct acccttacga
     3601 tgtaccggat tacgcagaat tcggatccgg tggaggcggg tctggaggcg ggggtagtgg
     3661 cgggggtgga agcgggggtg gaggcgggtc gggtggcgga ggtagcggag gcggtggaag
     3721 tggaggcggt ggaagtggtg gaggaggttc tggtggcgga ggtagcggtg gcggaggtag
     3781 cggaggcggt ggaagtggtg gcggaggtag cggaggcggt ggatcggcgc gccgttctag
     3841 aaagttcacc atagtttttc cacacaacca aaaaggaaac tggaaaaatg ttccttctaa
     3901 ttaccattat tgcccgtcaa gctcagattt aaattggcat aatgacttaa taggcacagc
     3961 cttacaagtc aaaatgccca agagtcacaa ggctattcaa gcagacggtt ggatgtgtca
     4021 tgcttccaaa tgggtcacta cttgtgattt ccgctggtat ggaccgaagt atataacaca
     4081 gtccatccga tccttcactc catctgtaga acaatgcaag gaaagcattg aacaaacgaa
     4141 acaaggaact tggctgaatc caggcttccc tcctcaaagt tgtggatatg caactgtgac
     4201 ggatgccgaa gcagtgattg tccaggtgac tcctcaccat gtgctggttg atgaatacac
     4261 aggagaatgg gttgattcac agttcatcaa cggaaaatgc agcaattaca tatgccccac
     4321 tgtccataac tctacaacct ggcattctga ctataaggtc aaagggctat gtgattctaa
     4381 cctcatttcc atggacatca ccttcttctc agaggacgga gagctatcat ccctgggaaa
     4441 ggagggcaca gggttcagaa gtaactactt tgcttatgaa actggaggca aggcctgcaa
     4501 aatgcaatac tgcaagcatt ggggagtcag actcccatca ggtgtctggt tcgagatggc
     4561 tgataaggat ctctttgctg cagccagatt ccctgaatgc ccagaagggt caagtatctc
     4621 tgctccatct cagacctcag tggatgtaag tctaattcag gacgttgaga ggatcttgga
     4681 ttattccctc tgccaagaaa cctggagcaa aatcagagcg ggtcttccaa tctctccagt
     4741 ggatctcagc tatcttgctc ctaaaaaccc aggaaccggt cctgctttca ccataatcaa
     4801 tggtacccta aaatactttg agaccagata catcagagtc gatattgctg ctccaatcct
     4861 ctcaagaatg gtcggaatga tcagtggaac taccacagaa agggaactgt gggatgactg
     4921 ggcaccatat gaagacgtgg aaattggacc caatggagtt ctgaggacca gttcaggata
     4981 taagtttcct ttatacatga ttggacatgg tatgttggac tccgatcttc atcttagctc
     5041 aaaggctcag gtgttcgaac atcctcacat tcaagacgct gcttcgcaac ttcctgatga
     5101 tgagagttta ttttttggtg atactgggct atccaaaaat ccaatcgagc ttgtagaagg
     5161 ttggttcagt agttggaaaa gctctattgc ctcttctaga ggaagtggaa ccacttcagg
     5221 tactacccgt cttctatctg ggcacacgtg tttcacgttg acaggtttgc ttgggacgct
     5281 agtaaccatg ggcttgctga cttagctcga gtctagagga tccctccccc cccccctaac
     5341 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5401 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5461 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5521 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     5581 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     5641 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     5701 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     5761 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     5821 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     5881 cgatgataat atggccacac atatggccca gtccaagcac ggcctgacca aggagatgac
     5941 catgaagtac cgcatggagg gctgcgtgga cggccacaag ttcgtgatca ccggcgaggg
     6001 catcggctac cccttcaagg gcaagcaggc catcaacctg tgcgtggtgg agggcggccc
     6061 cttgcccttc gccgaggaca tcttgtccgc cgccttcatg tacggcaacc gcgtgttcac
     6121 cgagtacccc caggacatcg tcgactactt caagaactcc tgccccgccg gctacacctg
     6181 ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc tgcaacgccg acatcaccgt
     6241 gagcgtggag gagaactgca tgtaccacga gtccaagttc tacggcgtga acttccccgc
     6301 cgacggcccc gtgatgaaga agatgaccga caactgggag ccctcctgcg agaagatcat
     6361 ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc atgtacctgc tgctgaagga
     6421 cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag gccaagtccg tgccccgcaa
     6481 gatgcccgac tggcacttca tccagcacaa gctgacccgc gaggaccgca gcgacgccaa
     6541 gaaccagaag tggcacctga ccgagcacgc catcgcctcc ggctccgcct tgccctgaat
     6601 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6661 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6721 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6781 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6841 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6901 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6961 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7021 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7081 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7141 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7201 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7261 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7321 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7381 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7441 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7501 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7561 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac
     7621 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7681 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7741 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8041 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     8101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8341 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8521 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9421 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9481 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9541 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9601 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9661 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc
     9721 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9781 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9841 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9901 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9961 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10021 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    10081 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    10141 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10201 tatgtactac aaacttagta gt
//
