LOCUS       sequence-420337-       11174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of rKIF5B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11174)
  AUTHORS   Jiao H, Jiang D, Hu X, Du W, Ji L, Yang Y, Li X, Sho T, Wang X, Li 
            Y, Wu YT, Wei YH, Hu X, Yu L
  TITLE     Mitocytosis, a migrasome-mediated mitochondrial quality-control 
            process.
  JOURNAL   Cell. 2021 May 27;184(11):2896-2910.e13. doi: 
            10.1016/j.cell.2021.04.027.
  PUBMED    34048705
REFERENCE   2  (bases 1 to 11174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04"; journalName: "Cell"; date: "2021-05-27- 
            27"; volume: "184"; issue: "11"; pages: "2896-2910.e13"
COMMENT     Sequence Label: pB-Hygro-rKIF5B-GFP
FEATURES             Location/Qualifiers
     source          1..11174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..656
                     /label=chicken beta-actin promoter
     intron          657..1672
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1596..1618
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1680..1699
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1763..1772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4664..4666,4667..4669,4670..5380)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4664..4666,4667..4669,4670..5380)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4709..4730)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4970..4989)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5317..5338
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5428..5445)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5434..5658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5743..5860
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5906..5939
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   6110..6172
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6196..6214)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6235..6251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6235..6251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6248..6270)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6259..6275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6283..6289,6290..6307,6308..6313))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6328..6349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6466..6483)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6637..7225)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6717..6736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7396..8187,8188..8256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8019..8038
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8257..8361)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8387..8842)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8524..8545)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8736..8755
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8969..8991
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8983..9000
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8984..9000
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9007..9026
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9007..9025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9044..9078
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(9560..10585)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9560..10585)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10604..11103)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10652..10671)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     11045..11068
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    11126..11159
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      541 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      601 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      661 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      721 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      781 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
      841 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
      901 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
      961 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
     1021 gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1081 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     1141 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     1201 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     1261 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     1321 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     1381 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     1501 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     1561 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     1621 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     1681 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcctcg acggtatcga
     1741 taagctatca caagtttgta cagccaccat ggcggacccg gcggagtgca acatcaaagt
     1801 gatgtgtcgc ttcagacctc tcaacgagtc tgaagtgaac cgcggcgaca agtatgtcgc
     1861 caagttccag ggagaagaca cggtgatgat tgcgtccaag ccttatgcat ttgatcgtgt
     1921 gttccagtca agcacatctc aagagcaagt atacaacgat tgtgcaaaga agattgttaa
     1981 agatgttctt gagggctata atggaacaat atttgcatat ggacaaacat catctggaaa
     2041 gacccacaca atggagggta aacttcatga tccagaaggc atggggatta ttccaagaat
     2101 agtgcaagat atttttaatt atatttactc catggatgaa aatttggaat ttcatattaa
     2161 ggtttcatat tttgaaatat atttggataa gataagggac ctgttagatg tttcaaagac
     2221 taacctttca gtccatgaag acaaaaaccg tgttccctat gtaaaggggt gcacagagcg
     2281 ttttgtgtgt agtccagatg aagttatgga taccatagat gaagggaaat ccaacagaca
     2341 cgtagcagtt acaaatatga atgaacatag ctctaggagt cacagtatat ttcttatcaa
     2401 tgtaaagcaa gagaatacac agacagaaca gaaactcagt gggaagcttt atctggttga
     2461 tttagctgga agtgaaaagg ttagtaaaac tggagctgag ggtgccgtgc tggatgaagc
     2521 taagaacatc aacaagtcac tttctgcact tggaaatgtc atttctgctt tggcagaagg
     2581 cagtacctat gttccttatc gagacagtaa aatgaccaga attcttcaag attcattagg
     2641 tggcaactgt aggaccacta ttgtcatttg ctgctctccg tcatcttaca atgagtctga
     2701 gacaaagtca acactcctct ttggtcaaag ggccaaaaca ataaagaaca cagtctgtgt
     2761 caacgtagag ttaactgcag agcagtggaa aaagaagtat gaaaaagaaa aggaaaaaaa
     2821 taagactcta cggaacacca ttcagtggct ggaaaacgag ctcaaccgat ggcgtaacgg
     2881 ggaaacagtg cctattgatg agcagtttga caaagagaag gctaatttgg aagccttcac
     2941 agcagataaa gatgttgcta ttaccaatga caaaccggct gctgcaatcg gaatggctgg
     3001 tagttttaca gatgctgaga gaagaaagtg tgaagaagaa attgctaaat tgtataaaca
     3061 acttgatgac aaggatgaag agattaacca acagagccaa ctggtggaga aattgaagac
     3121 acaaatgttg gatcaggaag agcttctggc atcaaccaga agggatcaag ataatatgca
     3181 agctgaactg aatcgcctcc aagcagaaaa tgatgcttct aaagaagaag tcaaagaagt
     3241 tttacaggcc ttagaggagc tggctgttaa ttatgatcag aagtctcagg aagttgaaga
     3301 caaaacaaag gaatatgaat tgcttagtga tgaattgaat caaaaatctg caactttagc
     3361 aagtattgat gctgagcttc agaagctgaa ggaaatgacc aaccaccaga agaaacgagc
     3421 agctgaaatg atggcatcat tactaaaaga ccttgcagaa ataggaattg ctgtgggaaa
     3481 taatgatgta aagcaaccgg aggggactgg tatgatagat gaagagttta ctgttgcaag
     3541 gctctacatt agcaaaatga aatcagaagt aaaaaccatg gtgaaacgct gcaaacagct
     3601 agaaagcaca cagactgaga gcaacaaaaa aatggaagaa aatgagaagg agttagcagc
     3661 atgccagctt cgtatctccc aacatgaagc caaaatcaag tcactgactg aataccttca
     3721 aaatgtagaa caaaagaaga gacagctgga ggaatctgtg gattcccttg gtgaggagct
     3781 agtccaactt cgagcacaag agaaagtcca tgaaatggaa aaagagcact tgaacaaggt
     3841 tcagactgca aatgaagtca agcaagctgt tgaacagcag atccagagtc acagagaaac
     3901 ccaccaaaaa caaatcagta gtttacgaga tgaagttgag gcaaaggaaa agctaatcac
     3961 tgatctccaa gaccaaaacc agaagatggt gttggaacag gagcggctaa gggtggagca
     4021 tgagaggcta aaggctgtag accaggagaa gagcaggaag ctgcatgagc tcacggttat
     4081 gcaagacaga cgagaacaag caaggcaaga tttgaagggg ttggaagaga cggtggcaaa
     4141 agaacttcag actttacaca acctgcgcaa gctctttgtt caggacttgg ctaccagggt
     4201 gaaaaagagc gctgaggtgg actcagacga cactggcggc agtgctgcac agaagcagaa
     4261 aatctccttc cttgaaaaca atctggagca gctcaccaaa gtgcacaagc agttggtgcg
     4321 tgataatgca gatcttcgct gtgaacttcc taagttagag aaacgactta gagctactgc
     4381 agagagagtg aaagctttgg agtcagccct gaaagaagcc aaagaaaatg catctcgaga
     4441 ccgcaaacgc tatcagcaag aagtagacag gataaaggaa gcagtcaggt caaagaatat
     4501 ggccagaagg ggacactctg cacagattgc aaaacctatc cgtcctggac agcatccagc
     4561 agcctctcca actcaccccg gtgcagttcg tggaggaggc tcatttgttc agaacaacca
     4621 gccagtgggg cttcgaggtg gtggaggcaa gcaggcgccc gggatggtga gcaagggcga
     4681 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4741 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4801 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     4861 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4921 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4981 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     5041 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     5101 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     5161 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     5221 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     5281 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     5341 cgccgccggg atcactctcg gcatggacga gctatacaag ggatccacgc gtctcgagtt
     5401 aacggtacct ctagagtaat tcgctagcct cgactgtgcc ttctagttgc cagccatctg
     5461 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5521 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5581 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5641 atgcggtggg ctctatggct tctgaggcgg ttaattaaaa taaaactaga gcgggcccgg
     5701 tactaaccaa actggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
     5761 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     5821 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
     5881 ttgtctgcag agcggccgcg tcgagataac ttcgtataat gtatgctata cgaagttatg
     5941 ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca
     6001 taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa
     6061 tatctattca aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat
     6121 tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag ggttaaagag
     6181 ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     6241 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6301 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6421 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6481 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6541 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6601 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6661 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6721 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6781 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6841 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6901 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6961 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7021 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7081 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7141 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7201 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7261 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7321 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7381 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7441 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7501 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7561 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7621 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7681 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7741 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7801 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7861 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7921 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7981 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8041 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8101 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8161 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8221 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8281 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8341 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     8401 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     8461 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     8521 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     8581 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     8641 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     8701 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     8761 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     8821 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     8881 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     8941 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     9001 gaattgtaat acgactcact atagggcgaa ttgggtacct taaccctaga aagatagtct
     9061 gcgtaaaatt gacgcatgca ttcttgaaat attgctctct ctttctaaat agcgcgaatc
     9121 cgtcgctgtg catttaggac atctcagtcg ccgcttggag ctcccgtgag gcgtgcttgt
     9181 caatgcggta agtgtcactg attttgaact ataacgaccg cgtgagtcaa aatgacgcat
     9241 gattatcttt tacgtgactt ttaagattta actcatacga taattatatt gttatttcat
     9301 gttctactta cgtgataact tattatatat atattttctt gttatagata tccaactatg
     9361 aaacattatc ataaagcaat agtaaagagc cttcagtaaa gagcaggcat ttatctaatc
     9421 ccaccccacc cccacccccg tagctccaat ccttccattc aaaatgtagg tactctgttc
     9481 tcacccttct taacaaagta tgacaggaaa aacttccatg tggacatctt tattgtttaa
     9541 tagatcatca atttctcgac tattcctttg ccctcggacg agtgctgggg cgtcggtttc
     9601 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     9661 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     9721 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     9781 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     9841 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     9901 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     9961 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
    10021 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
    10081 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
    10141 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
    10201 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
    10261 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
    10321 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
    10381 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
    10441 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
    10501 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
    10561 tcgcggtgag ttcaggcttt ttcatggtgg cggctggatc ggtcgaaagg cccggagatg
    10621 aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc
    10681 cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg cccccggacc
    10741 caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc
    10801 cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga
    10861 gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact
    10921 tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt
    10981 ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg tagcgccaag
    11041 tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc gcctccccta
    11101 cccggtagaa ttcatcgatg atatcataac ttcgtataat gtatgctata cgaagttatg
    11161 cggccctcga ggtc
//
