LOCUS       sequence-42034-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: USP08 (2GWF)
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             543..560
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(613..631)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      627..674
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcctctga atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggttccg cgtggtagtg gagcaatcac agcaaaggaa
      121 ctatacacaa tgatgacgga taaaaacatc agcttgatta taatggatgc tcgaagaatg
      181 caggattatc aggattcctg tattttacat tctctcagtg ttcctgaaga agccatcagt
      241 ccaggagtca ctgctagttg gattgaagca cacctgccag atgattctaa agacacatgg
      301 aagaagaggg ggaatgtgga gtatgtggta cttcttgact ggtttagttc tgccaaagat
      361 ttacagattg gaacaactct ccggagtctg aaagatgcac ttttcaagtg ggaaagtaaa
      421 actgtcctgc gcaatgagcc tttggtttta gagggaggct atgaaaactg gctcctttgt
      481 tatccccagt atacaacaaa tgctaaggtc acttgatgac gaagcttgcg gccgcactcg
      541 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      601 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      661 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      721 ta
//
