LOCUS       sequence-420387-       12088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for Uni-Vector prime editing system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Huang CH, Chiu SY, Chou YC, Wu KJ
  TITLE     A refined Uni-vector prime editing system improves genome editing 
            outcomes in mammalian cells.
  JOURNAL   Biotechnol J. 2024 Feb;19(2):e2300353. doi: 10.1002/biot.202300353.
  PUBMED    38403398
REFERENCE   2  (bases 1 to 12088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2024-02"; volume: "19(2):e2300353. doi"; pages: " 
            10.1002/biot.202300353"
COMMENT     Sequence Label: pePEmax-pPuro
FEATURES             Location/Qualifiers
     source          1..12088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..123
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        130..378
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     130..150
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     301..320
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2470..2681
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2689..2698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2701..2721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2725..6825
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6883..6903
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9001..9021
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9031..9087
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9088..9684
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9581..9601
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9691..10279
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9744..9764)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10162..10173)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10286..10334
                     /note="synthetic polyadenylation signal"
     promoter        10356..10460
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10461..10529,10530..11321)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10679..10698)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11492..12080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11981..12000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagtttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
       61 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      121 tttactagtg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
      181 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
      241 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      301 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      361 tggaaaggac gaaacaccgg agacggtgtt gtaaatgagc acacaaaata cacatgctaa
      421 aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa taactttagt
      481 atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat gagcaaaata
      541 aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat agctactcca
      601 ccacttgttc ctaagcggtc agctcctgct tcaatcattt tttgagcatc ttcaaatgtt
      661 ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt cattagttta
      721 acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac aaattctaat
      781 ccagcttcaa cagctatttc acaagctttc atgatttctt cttttgttaa taaacaattt
      841 tccataatac atttaacaac atgtgatcca gctgcttttt ttacagcttt catgtcttct
      901 aaaactaatt cataattttt gtcttttaat gcaccaatat ttaataccat atcaatttct
      961 gttgcaccat ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt tgtgcatgca
     1021 cctagaggaa aacctacaac atttgttatt cctacatttg tgccttttaa taattcttta
     1081 caatagcttg ttcaatatga attaacacaa actgttgcaa aatcaaattc aattgcttca
     1141 tcacataatt gtttaatttc agctttcgta gcatcttgtt ttaataatgt gtgatctata
     1201 tatttgttta gtttcatttt ttctcctata tattcatttt taattttaat tctttaataa
     1261 tttcgtctac tttaacttta gcgttttgaa cagattcacc aacacctata aaataaattt
     1321 ttagtttagg ttcagttcca cttgggcgaa cagcaaatca tgacttatct tctaaataaa
     1381 attttagtaa gtcttgtcct ggcatattat acattccatc gatgtagtct tcaacattaa
     1441 caactttaag tccagcaatt tgagttaagg gtgttgctct caatgatttc attaatggtt
     1501 caatttttaa tttcttttct tctggtttaa aattcaagtt taaagtgaaa gtgtaatatg
     1561 cacccatttc tttaaataaa tcttctaaat agtctactaa tgttttattt tgttttttat
     1621 aaaatcaagc agcctctgct attaatatag aagcttgtat tccatcttta tctctagctg
     1681 agtcatcaat tacatatcca taactttctt cataagcaaa aacaaaattt aatccgttat
     1741 cttcttcttt agcaatttct ctacccattc atttaaatcc agttaaagtt tttacaatat
     1801 taactccata tttttcatga gcgattctat cacccaaatc acttgttaca aaacttgaat
     1861 atagagccgg attttttgga atgctattta agcgttttag atttgataat tttcaatcaa
     1921 ttaaaattgg tcctgtttga tttccatcta atcttacaaa atgaccatca tgttttattg
     1981 ccattccaaa tctgtcagca tctgggtcat tcataataat aatatctgca tcatgtttaa
     2041 taccatattc aagcggtatt tttcatgcag gatcaaattc tggatttgga tttacaacat
     2101 ttttaaatgt ttcatcttca aatgcatgct cttcaacctc aataacgtta tatcctgatt
     2161 cacgtaatat ttttggggta aatttagttc ctgttccatt aactgcgcta aaaataattt
     2221 ttaaatcttt tttagcttct tgctcttttt tgtacgtctc tgtttaagag ctatgctgga
     2281 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     2341 tcggtgcata ggcaggtgta taatagcacc tgctatacgc ggttctatct agttacgcgt
     2401 taaaccaact agaatttttt gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2461 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2521 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2581 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2641 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggtgc caccatggcc
     2701 ccaaagaaga agcggaaggt cggtgacaag aagtacagca tcggcctgga catcggcacc
     2761 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
     2821 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
     2881 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
     2941 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
     3001 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
     3061 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
     3121 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
     3181 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
     3241 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
     3301 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
     3361 gccagactga gcaagagcag aaagctggaa aatctgatcg cccagctgcc cggcgagaag
     3421 aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
     3481 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
     3541 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
     3601 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
     3661 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
     3721 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
     3781 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
     3841 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
     3901 aagagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
     3961 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
     4021 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
     4081 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
     4141 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
     4201 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
     4261 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
     4321 atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc
     4381 aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag
     4441 tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca
     4501 taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag
     4561 gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag
     4621 gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg
     4681 cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag
     4741 cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc
     4801 atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg
     4861 tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt
     4921 aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg
     4981 cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga
     5041 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
     5101 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
     5161 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
     5221 tccgactacg atgtggacgc tatcgtgcct cagagctttc tgaaggacga ctccatcgac
     5281 aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa
     5341 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
     5401 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
     5461 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
     5521 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
     5581 aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac
     5641 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
     5701 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac
     5761 aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc
     5821 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
     5881 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
     5941 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
     6001 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
     6061 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
     6121 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
     6181 tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc
     6241 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
     6301 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
     6361 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
     6421 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
     6481 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
     6541 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
     6601 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
     6661 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
     6721 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
     6781 ggcctgtacg agacacggat cgacctgtct cagctgggag gtgactccgg cggaagctct
     6841 ggtggcagca agcggaccgc cgacggctct gaattcgaga gccctaagaa gaaaagaaag
     6901 gtgagcggag gctctagcgg cggaagcacc ctgaacattg aagacgagta tagactgcat
     6961 gaaacaagca aggaacccga cgtgtccctg ggctccacct ggctgtccga ctttccccag
     7021 gcctgggccg agacaggagg aatgggcctg gccgtgcggc aggcacccct gatcatccct
     7081 ctgaaggcca cctctacacc cgtgagcatc aagcagtacc ctatgtctca ggaggccaga
     7141 ctgggcatca agcctcacat ccagaggctg ctggaccagg gcatcctggt gccatgccag
     7201 agcccctgga acacaccact gctgcccgtg aagaagccag gcaccaatga ctatagaccc
     7261 gtgcaggatc tgagagaggt gaacaagagg gtggaggata tccaccccac cgtgcccaac
     7321 ccttacaatc tgctgtccgg cctgccccct tctcaccagt ggtatacagt gctggacctg
     7381 aaggatgcct tcttttgtct gagactgcac cctaccagcc agccactgtt cgcctttgag
     7441 tggagggacc ctgagatggg catctctggc cagctgacct ggacacgcct gcctcagggc
     7501 ttcaagaata gcccaacact gtttaacgag gccctgcacc gcgacctggc agatttccgg
     7561 atccagcacc cagatctgat cctgctgcag tacgtggacg atctgctgct ggccgccacc
     7621 agcgagctgg attgccagca gggaacacgc gccctgctgc agaccctggg aaacctggga
     7681 tatagggcat ccgccaagaa ggcccagatc tgtcagaagc aagtgaagta cctgggctat
     7741 ctgctgaagg agggccagag atggctgaca gaggccagga aggagacagt gatgggccag
     7801 ccaacaccca agaccccaag acagctgagg gagttcctgg gcaaagcagg attttgcagg
     7861 ctgttcatcc caggattcgc agagatggca gcacctctgt acccactgac caagccgggc
     7921 accctgttta attggggccc tgaccagcag aaggcctatc aggagatcaa gcaggccctg
     7981 ctgacagcac cagccctggg cctgccagac ctgaccaagc ctttcgagct gtttgtggat
     8041 gagaagcagg gctacgccaa gggcgtgctg acccagaagc tgggaccatg gaggaggccc
     8101 gtggcctatc tgtccaagaa gctggaccca gtggcagcag gatggccacc atgcctgagg
     8161 atggtggcag caatcgccgt gctgacaaag gatgccggca agctgaccat gggacagcca
     8221 ctggtcatcc tggcaccaca cgcagtggag gccctggtga agcagcctcc agatcgctgg
     8281 ctgtctaacg cccggatgac acactaccag gccctgctgc tggacaccga tcgcgtgcag
     8341 tttggccctg tggtggccct gaatccagcc accctgctgc ctctgccaga ggagggcctg
     8401 cagcacaact gtctggacat cctggcagag gcacacggaa caaggccaga cctgaccgat
     8461 cagcccctgc ctgacgccga tcacacatgg tataccgatg gaagctccct gctgcaggag
     8521 ggccagagga aggcaggagc agcagtgacc acagagacag aagtgatctg ggccaaggcc
     8581 ctgccagcag gcacatccgc ccagcgggcc gagctgatcg ccctgaccca ggccctgaag
     8641 atggccgagg gcaagaagct gaacgtgtac acagactcca gatatgcctt cgccaccgca
     8701 cacatccacg gagagatcta caggcgccgg ggctggctga cctctgaggg caaggagatc
     8761 aagaacaagg atgagatcct ggccctgctg aaggccctgt ttctgcccaa gcggctgagc
     8821 atcatccact gtcctggaca ccagaaggga cactccgccg aggcaagggg caatcggatg
     8881 gccgaccagg ccgccagaaa ggctgctatt actgaaactc ccgacacttc cactctgctg
     8941 attgaaaact cctccccttc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
     9001 cccaagaaga agaggaaagt cggatccggc gcaacaaact tctctctgct gaaacaagcc
     9061 ggagatgtcg aagagaatcc tggaccgacc gagtacaagc ccacggtgcg cctcgccacc
     9121 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     9181 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     9241 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     9301 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     9361 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     9421 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc
     9481 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     9541 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     9601 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     9661 atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa
     9721 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     9781 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     9841 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     9901 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9961 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    10021 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    10081 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
    10141 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    10201 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    10261 ctttgggccg cctccccgca cgcgtaataa aagatcttta ttttcattag atctgtgtgt
    10321 tggttttttg tgtgctctag attcggggaa atgtgcgcgg aacccctatt tgtttatttt
    10381 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    10441 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    10501 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    10561 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    10621 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10681 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    10741 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10801 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10861 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10921 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10981 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    11041 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    11101 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    11161 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    11221 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    11281 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    11341 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    11401 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    11461 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    11521 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    11581 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    11641 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    11701 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    11761 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    11821 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    11881 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    11941 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    12001 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    12061 gggggcggag cctatggaaa aacgccac
//
