LOCUS       sequence-420388-       12048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for Uni-Vector prime editing system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Huang CH, Chiu SY, Chou YC, Wu KJ
  TITLE     A refined Uni-vector prime editing system improves genome editing 
            outcomes in mammalian cells.
  JOURNAL   Biotechnol J. 2024 Feb;19(2):e2300353. doi: 10.1002/biot.202300353.
  PUBMED    38403398
REFERENCE   2  (bases 1 to 12048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2024-02"; volume: "19(2):e2300353. doi"; pages: " 
            10.1002/biot.202300353"
COMMENT     Sequence Label: pPE3-pPuro
FEATURES             Location/Qualifiers
     source          1..12048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..123
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        130..378
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     130..150
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     301..320
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2433..2644
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2652..2661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2664..2684
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2688..5201,5202..5204,5205..6788)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2688..6788
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8961..8981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8991..9047
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9048..9644
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9541..9561
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9651..10239
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9704..9724)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10122..10133)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10246..10294
                     /note="synthetic polyadenylation signal"
     promoter        10316..10420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10421..10489,10490..11281)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10639..10658)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11452..12040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11941..11960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagtttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
       61 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      121 tttactagtg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
      181 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
      241 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      301 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      361 tggaaaggac gaaacaccgg agacggtgtt gtaaatgagc acacaaaata cacatgctaa
      421 aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa taactttagt
      481 atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat gagcaaaata
      541 aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat agctactcca
      601 ccacttgttc ctaagcggtc agctcctgct tcaatcattt tttgagcatc ttcaaatgtt
      661 ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt cattagttta
      721 acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac aaattctaat
      781 ccagcttcaa cagctatttc acaagctttc atgatttctt cttttgttaa taaacaattt
      841 tccataatac atttaacaac atgtgatcca gctgcttttt ttacagcttt catgtcttct
      901 aaaactaatt cataattttt gtcttttaat gcaccaatat ttaataccat atcaatttct
      961 gttgcaccat ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt tgtgcatgca
     1021 cctagaggaa aacctacaac atttgttatt cctacatttg tgccttttaa taattcttta
     1081 caatagcttg ttcaatatga attaacacaa actgttgcaa aatcaaattc aattgcttca
     1141 tcacataatt gtttaatttc agctttcgta gcatcttgtt ttaataatgt gtgatctata
     1201 tatttgttta gtttcatttt ttctcctata tattcatttt taattttaat tctttaataa
     1261 tttcgtctac tttaacttta gcgttttgaa cagattcacc aacacctata aaataaattt
     1321 ttagtttagg ttcagttcca cttgggcgaa cagcaaatca tgacttatct tctaaataaa
     1381 attttagtaa gtcttgtcct ggcatattat acattccatc gatgtagtct tcaacattaa
     1441 caactttaag tccagcaatt tgagttaagg gtgttgctct caatgatttc attaatggtt
     1501 caatttttaa tttcttttct tctggtttaa aattcaagtt taaagtgaaa gtgtaatatg
     1561 cacccatttc tttaaataaa tcttctaaat agtctactaa tgttttattt tgttttttat
     1621 aaaatcaagc agcctctgct attaatatag aagcttgtat tccatcttta tctctagctg
     1681 agtcatcaat tacatatcca taactttctt cataagcaaa aacaaaattt aatccgttat
     1741 cttcttcttt agcaatttct ctacccattc atttaaatcc agttaaagtt tttacaatat
     1801 taactccata tttttcatga gcgattctat cacccaaatc acttgttaca aaacttgaat
     1861 atagagccgg attttttgga atgctattta agcgttttag atttgataat tttcaatcaa
     1921 ttaaaattgg tcctgtttga tttccatcta atcttacaaa atgaccatca tgttttattg
     1981 ccattccaaa tctgtcagca tctgggtcat tcataataat aatatctgca tcatgtttaa
     2041 taccatattc aagcggtatt tttcatgcag gatcaaattc tggatttgga tttacaacat
     2101 ttttaaatgt ttcatcttca aatgcatgct cttcaacctc aataacgtta tatcctgatt
     2161 cacgtaatat ttttggggta aatttagttc ctgttccatt aactgcgcta aaaataattt
     2221 ttaaatcttt tttagcttct tgctcttttt tgtacgtctc tgtttaagag ctatgctgga
     2281 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     2341 tcggtgcata ggcaggtgta taatagcacc tgctatattt tttgctagct aggtcttgaa
     2401 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2461 cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt ggcgcggggt
     2521 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2581 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2641 acaggaccgg tgccaccatg gccccaaaga agaagcggaa ggtcggtgac aagaagtaca
     2701 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     2761 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2821 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2881 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     2941 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     3001 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     3061 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     3121 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     3181 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     3241 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     3301 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     3361 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     3421 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     3481 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     3541 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     3601 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     3661 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     3721 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3781 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3841 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3901 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     3961 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     4021 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     4081 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     4141 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     4201 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     4261 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     4321 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     4381 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     4441 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     4501 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     4561 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     4621 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     4681 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     4741 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4801 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4861 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4921 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     4981 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     5041 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     5101 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     5161 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     5221 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     5281 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     5341 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     5401 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     5461 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     5521 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     5581 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     5641 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     5701 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     5761 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5821 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5881 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     5941 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     6001 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     6061 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     6121 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     6181 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     6241 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     6301 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     6361 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     6421 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     6481 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     6541 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     6601 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     6661 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     6721 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6781 gaggtgactc tggaggatct agcggaggat cctctggcag cgagacacca ggaacaagcg
     6841 agtcagcaac accagagagc agtggcggca gcagcggcgg cagcagcacc ctaaatatag
     6901 aagatgagta tcggctacat gagacctcaa aagagccaga tgtttctcta gggtccacat
     6961 ggctgtctga ttttcctcag gcctgggcgg aaaccggggg catgggactg gcagttcgcc
     7021 aagctcctct gatcatacct ctgaaagcaa cctctacccc cgtgtccata aaacaatacc
     7081 ccatgtcaca agaagccaga ctggggatca agccccacat acagagactg ttggaccagg
     7141 gaatactggt accctgccag tccccctgga acacgcccct gctacccgtt aagaaaccag
     7201 ggactaatga ttataggcct gtccaggatc tgagagaagt caacaagcgg gtggaagaca
     7261 tccaccccac cgtgcccaac ccttacaacc tcttgagcgg gctcccaccg tcccaccagt
     7321 ggtacactgt gcttgattta aaggatgcct ttttctgcct gagactccac cccaccagtc
     7381 agcctctctt cgcctttgag tggagagatc cagagatggg aatctcagga caattgacct
     7441 ggaccagact cccacagggt ttcaaaaaca gtcccaccct gtttaatgag gcactgcaca
     7501 gagacctagc agacttccgg atccagcacc cagacttgat cctgctacag tacgtggatg
     7561 acttactgct ggccgccact tctgagctag actgccaaca aggtactcgg gccctgttac
     7621 aaaccctagg gaacctcggg tatcgggcct cggccaagaa agcccaaatt tgccagaaac
     7681 aggtcaagta tctggggtat cttctaaaag agggtcagag atggctgact gaggccagaa
     7741 aagagactgt gatggggcag cctactccga agacccctcg acaactaagg gagttcctag
     7801 ggaaggcagg cttctgtcgc ctcttcatcc ctgggtttgc agaaatggca gcccccctgt
     7861 accctctcac caaaccgggg actctgttta attggggccc agaccaacaa aaggcctatc
     7921 aagaaatcaa gcaagctctt ctaactgccc cagccctggg gttgccagat ttgactaagc
     7981 cctttgaact ctttgtcgac gagaagcagg gctacgccaa aggtgtccta acgcaaaaac
     8041 tgggaccttg gcgtcggccg gtggcctacc tgtccaaaaa gctagaccca gtagcagctg
     8101 ggtggccccc ttgcctacgg atggtagcag ccattgccgt actgacaaag gatgcaggca
     8161 agctaaccat gggacagcca ctagtcattc tggcccccca tgcagtagag gcactagtca
     8221 aacaaccccc cgaccgctgg ctttccaacg cccggatgac tcactatcag gccttgcttt
     8281 tggacacgga ccgggtccag ttcggaccgg tggtagccct gaacccggct acgctgctcc
     8341 cactgcctga ggaagggctg caacacaact gccttgatat cctggccgaa gcccacggaa
     8401 cccgacccga cctaacggac cagccgctcc cagacgccga ccacacctgg tacacggatg
     8461 gaagcagtct cttacaagag ggacagcgta aggcgggagc tgcggtgacc accgagaccg
     8521 aggtaatctg ggctaaagcc ctgccagccg ggacatccgc tcagcgggct gaactgatag
     8581 cactcaccca ggccctaaag atggcagaag gtaagaagct aaatgtttat actgatagcc
     8641 gttatgcttt tgctactgcc catatccatg gagaaatata cagaaggcgt gggtggctca
     8701 catcagaagg caaagagatc aaaaataaag acgagatctt ggccctacta aaagccctct
     8761 ttctgcccaa aagacttagc ataatccatt gtccaggaca tcaaaaggga cacagcgccg
     8821 aggctagagg caaccggatg gctgaccaag cggcccgaaa ggcagccatc acagagactc
     8881 cagacacctc taccctcctc atagaaaatt catcaccctc tggcggctca aaaagaaccg
     8941 ccgacggcag cgaattcgag cccaagaaga agaggaaagt cggatccggc gcaacaaact
     9001 tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgacc gagtacaagc
     9061 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     9121 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     9181 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     9241 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     9301 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     9361 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     9421 ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     9481 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     9541 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     9601 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc
     9661 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     9721 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     9781 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     9841 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     9901 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     9961 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
    10021 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
    10081 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
    10141 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
    10201 ctcagacgag tcggatctcc ctttgggccg cctccccgca cgcgtaataa aagatcttta
    10261 ttttcattag atctgtgtgt tggttttttg tgtgctctag attcggggaa atgtgcgcgg
    10321 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    10381 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    10441 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    10501 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    10561 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    10621 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    10681 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    10741 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    10801 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    10861 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    10921 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    10981 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    11041 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11101 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    11161 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    11221 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    11281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    11341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    11401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    11461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    11521 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    11581 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    11641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    11701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    11761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    11821 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    11881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    11941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    12001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccac
//
