LOCUS       sequence-420423-        6733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generation of Bacmid for insect cell expression of C17orf53 
            C-terminally fused to Strep-Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6733)
  TITLE     Pospiech Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBac1-C17orf53-Strep
FEATURES             Location/Qualifiers
     source          1..6733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..471
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(104..123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     313..334
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        497..601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(602..670,671..1462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(820..839)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1633..2221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2122..2141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2375..2392
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2526..2750
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2817..3350)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3539..3544,3545..3561,3562..3567))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3919..4010
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3935..3952
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             6015..6038
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    6238..6372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6275..6294)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6329..6348
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6401..6566
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
       61 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      121 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      181 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      241 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      301 acgttcttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      361 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      421 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
      481 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      541 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      601 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      661 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      721 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      781 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      841 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      901 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      961 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1021 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1081 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1141 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1201 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1261 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1321 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1381 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1441 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1501 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1561 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1621 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1681 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1741 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1801 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1861 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1921 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1981 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2041 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2101 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2161 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2221 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2281 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2341 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2401 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga
     2461 ccagccgcgt aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa
     2521 gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat
     2581 aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa
     2641 agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc
     2701 ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt
     2761 gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag
     2821 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
     2881 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
     2941 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
     3001 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
     3061 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     3121 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     3181 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     3241 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     3301 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
     3361 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     3421 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
     3481 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
     3541 tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt
     3601 gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa
     3661 cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta
     3721 cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc
     3781 gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga
     3841 aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
     3901 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
     3961 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
     4021 attcataccg tcccaccatc gggcgcggat cccggtccga agcgcgcgga attctcatgg
     4081 cgtgcagttt gcagaagctg tttgctgtgg aagaggagtt tgaagatgag gatttcttgt
     4141 ctgctgtgga ggatgcagag aaccggttta ctggctcact gcctgtgaat gctgggcgcc
     4201 tgagacctgt ctcttctagg ccacaggaga ctgtgcaggc acagtcctcc aggctgctgc
     4261 tgttacaccc cactgctccc tcagaggctt tgggcctgcc agacttggac ctctgcctcc
     4321 ctgcctccag cacgcccagt gctgacagcc gtccatcatg cataggagca gctcccctaa
     4381 ggcctgtctc tacttccagc agctggattg gcaatcagag aagagtgaca gtgacagaag
     4441 tgctcagaga gacagcaaga cctcagtcct cagccttaca ccccctactc acctttgaga
     4501 gccaacagca gcaagttggt ggctttgagg ggcctgaaca agacgaattt gataaagtcc
     4561 tggcaagcat ggagttggag gagcctggca tggagctgga atgtggagtc agcagtgagg
     4621 ccataccaat cctgcctgcc cagcagcggg agggttcagt attggctaaa aaagcccggg
     4681 tagttgatct gagtggatct tgccagaagg ggcctgtgcc tgccatccac aaagcgggta
     4741 tcatgtccgc ccaggatgag tctctagatc ctgtcatcca atgtaggact ccacgacccc
     4801 ccttgagacc tggtgctgtg ggtcaccttc ctgttccaac tgccttaaca gttcccactc
     4861 agcaactcca ctgggaagtc tgtccgcaac gctcccctgt tcaagcactt cagcctctcc
     4921 aagctgctag agggaccatt cagagcagcc ctcaaaatcg tttcccttgt cagccattcc
     4981 agtctccaag ttcctggtta agtggcaaag ctcatttacc cagacctcga actcccaact
     5041 caagctgttc tactccctca aggactagct ctggattatt tcctcggata cccttacaac
     5101 cgcaagctcc agtgtcttcc attgggtctc ctgttggtac cccaaaaggt ccccagggag
     5161 ctctgcagac acccatagtc accaaccacc tggtgcagct agtcactgct gccagccgga
     5221 caccccagca gcccacccat ccctccaccc gagccaaaac tcgccgtttc cctggcccag
     5281 ctgggatcct gcctcaccag cagagtggga gaagtctgga ggacatcatg gtttccgcgc
     5341 cccaaactcc aacccatggt gctctggcta aattccagac agagattgtt gctagttccc
     5401 aggcatctgt ggaggaggat tttgggcgag ggccctggct gaccatgaaa tccacgctag
     5461 gcctggatga gagagaccct agctgcttcc tctgtaccta cagcattgtc atggtgctgc
     5521 gcaaggcagc cctgaagcag cttcctagga acaaggtccc caacatggcg gtgatgatca
     5581 agtccctgac tcggagcaca atggacgcca gtgtggtttt caaggacccc acgggagaga
     5641 tgcaggggac ggtgcacagg ttgctgctgg agacgtgcca gaatgagctg aagcctggct
     5701 cagtgctgct gctgaagcag attggagtgt tttctccttc acttcgaaat cactacctca
     5761 acgtgacacc caacaacctg gtccatattt acagcccgga ttctggggat gggagcttcc
     5821 tcaagccatc tcagcccttc cccaaggatt cagggagctt ccagcatgat gtggctgcaa
     5881 agcccgagaa aggcttcaga acagcacaga acctagaggc agaggcgtcc cctgaggaag
     5941 aactcccaga agcagatgac ctggatggac tcctgagtga gcttcctgaa gacttcttct
     6001 gtgggaccag tagttggagc cacccgcagt tcgaaaaatg agtcgacgag ctcactagtc
     6061 gcggccgctt tcgaatctag agcctgcagt ctcgaggcat gcggtaccaa gcttgtcgag
     6121 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     6181 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     6241 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6301 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6361 catgtctgga tctgatcact gcttgagcct aggagatccg aaccagataa gtgaaatcta
     6421 gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc
     6481 ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca
     6541 gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc
     6601 aaacatcctg ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca
     6661 gaatgaaaat ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac
     6721 gcttatttgc agc
//
