LOCUS       sequence-420433-        8893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express tagged cyclin E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Konagaya Y, Rosenthal D, Ratnayeke N, Fan Y, Meyer T
  TITLE     An intermediate Rb-E2F activity state safeguards proliferation 
            commitment.
  JOURNAL   Nature. 2024 Jul;631(8020):424-431. doi: 10.1038/s41586-024-07554-2.
            Epub 2024 Jun 26.
  PUBMED    38926571
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07554-2"; journalName: "Nature"; date: 
            "2024-07"; volume: "631"; issue: "8020"; pages: "424-431"
COMMENT     Sequence Label: pCW-CCNE1-HA-puro
FEATURES             Location/Qualifiers
     source          1..8893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1794..1820
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1835..2345
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2264..2282
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2355..2951
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2355..2374)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2851..2871
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2952..3005
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3006..3623,3624..3629,3630..3665,3666..3668,3669..3704,
                     3705..3707,3708..3743,3744..3752)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3792..3812)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4210..4221)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4426..4659
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4731..4852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4768..4787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4822..4841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4892..5027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5027..5098
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5099..5167,5168..5959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6130..6718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6619..6638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6872..6889
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7006..7027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7042..7047,7048..7065,7066..7072)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7080..7096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7085..7107
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7104..7120
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7104..7120
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7139..7159
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7141..7159
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7187..7413
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7414..7594
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7641..7766
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8259..8492
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8677..8721
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat gccgagggag cgcagggagc gggatgcgaa ggagcgggac accatgaagg
      601 aggacggcgg cgcggagttc tcggctcgct ccaggaagag gaaggcaaac gtgaccgttt
      661 ttttgcagga tccagatgaa gaaatggcca aaatcgacag gacggcgagg gaccagtgtg
      721 ggagccagcc ttgggacaat aatgcagtct gtgcagaccc ctgctccctg atccccacac
      781 ctgacaaaga agatgatgac cgggtttacc caaactcaac gtgcaagcct cggattattg
      841 caccatccag aggctccccg ctgcctgtac tgagctgggc aaatagagag gaagtctgga
      901 aaatcatgtt aaacaaggaa aagacatact taagggatca gcactttctt gagcaacacc
      961 ctcttctgca gccaaaaatg cgagcaattc ttctggattg gttaatggag gtgtgtgaag
     1021 tctataaact tcacagggag accttttact tggcacaaga tttctttgac cggtacatgg
     1081 ccacgcagga gaatgttgta aaaactcttt tacagcttat tgggatttca tctttattta
     1141 ttgcagccaa acttgaggaa atctatcctc caaagttgca ccagtttgcg tatgtgacag
     1201 atggagcttg ttcaggagat gaaattctca ccatggaatt aatgattatg aaggccctta
     1261 agtggcgttt aagtcccctg actattgtgt cctggctgaa tgtatacatg caggttgcat
     1321 atctaaatga cttacatgaa gtgctactgc cgcagtatcc ccagcaaatc tttatacaga
     1381 ttgcagagct gttggatctc tgtgtcctgg atgttgactg ccttgaattt ccttatggta
     1441 tacttgctgc ttcggccttg tatcatttct cgtcatctga attgatgcaa aaggtttcag
     1501 ggtatcagtg gtgcgacata gagaactgtg tcaagtggat ggttccattt gccatggtta
     1561 taagggagac ggggagctca aaactgaagc acttcagggg cgtcgctgat gaagatgcac
     1621 acaacataca gacccacaga gacagcttgg atttgctgga caaagcccga gcaaagaaag
     1681 ccatgttgtc tgaacaaaat agggcttctc ctctccccag tgggctcctc accccgccac
     1741 agagcggtaa gaagcagagc agcgggccgg aaatggcgag cgctggggga tcctatccgt
     1801 acgacgtacc agactacgca tgagaattcg atccggggtt ggggttgcgc cttttccaag
     1861 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     1921 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     1981 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     2041 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     2101 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     2161 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     2221 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     2281 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     2341 cccagcaatt caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     2401 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     2461 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     2521 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     2581 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     2641 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     2701 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     2761 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     2821 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     2881 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     2941 agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg
     3001 gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg
     3061 gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
     3121 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
     3181 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
     3241 tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag
     3301 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
     3361 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
     3421 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
     3481 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
     3541 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
     3601 aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca
     3661 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
     3721 acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc aagatatcgt
     3781 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3841 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3901 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3961 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4021 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4081 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     4141 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4201 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4261 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4321 tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca atgacttaca
     4381 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4441 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4501 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4561 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4621 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     4681 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     4741 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4801 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4861 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4921 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4981 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt caaatatgta
     5041 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5101 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5161 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5221 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5281 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5341 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5401 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5461 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5521 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5581 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5641 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5701 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5761 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5821 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5881 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5941 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6001 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6841 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6901 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6961 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7021 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7081 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     7141 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     7201 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     7261 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     7321 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     7381 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     7441 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7501 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7561 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7621 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7681 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7741 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     7801 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7861 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     7921 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     7981 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8041 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8101 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8161 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8221 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8281 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8341 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8401 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8461 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8521 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8581 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8641 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     8701 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     8761 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     8821 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     8881 accccgaggg gac
//
