LOCUS       sequence-420434-        8679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  halo donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Brambati A, Sacco O, Porcella S, Heyza J, Kareh M, Schmidt JC, Sfeir
            A
  TITLE     RHINO directs MMEJ to repair DNA breaks in mitosis.
  JOURNAL   Science. 2023 Aug 11;381(6658):653-660. doi: 
            10.1126/science.adh3694. Epub 2023 Jul 13.
  PUBMED    37440612
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adh3694"; journalName: "Science"; date: 
            "2023-08-11- 11"; volume: "381"; issue: "6658"; pages: "653-660"
COMMENT     Sequence Label: HDR-HaloPOLQ
FEATURES             Location/Qualifiers
     source          1..8679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    237..270
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             279..1169
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1182..1202
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     polyA_signal    1924..2058
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1961..1980)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2015..2034
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2087..2252
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2436..2890
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2523..2542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2732..2753
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2916..3020
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3021..3089,3090..3881)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3239..3258)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4052..4640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4541..4560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4794..4811
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4945..5169
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5236..5769)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5958..5963,5964..5980,5981..5986))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    6494..6542
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6567..6586
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(6678..6787)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        6811..6902
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     6827..6844
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             7832..7897
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    7898..7931
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    7938..7986
                     /note="synthetic polyadenylation signal"
     CDS             complement(7987..8586)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8070..8090)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8567..8586
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(8593..8679,1..230))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(8607..8679,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ggatccataa
      241 cttcgtataa tgtatgctat acgaagttat ccggtaccat ggcagaaatc ggtactggct
      301 ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg
      361 gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt
      421 ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg
      481 gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca
      541 tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg
      601 gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat
      661 ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga
      721 ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt
      781 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
      841 attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
      901 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
      961 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
     1021 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
     1081 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
     1141 gctggctgtc gacgctcgag atttccggcg agccaaccac tgaggatctg tactttcaga
     1201 gcgataacgc gatcgcttcc ggtacgatga atcttctgcg tcggagtggg aaacggcggc
     1261 gttcagaatc aggctcagat tcgttctcgg gaagcggcgg tgacagcagt gccagccccc
     1321 agttcctctc cgggtccgtg ctgagcccgc cgcccggcct tggtcgctgc ctgaaggccg
     1381 cagctgcagg tgctccagga acccgggagg ccgcgggcaa cgggggcagc tccgccgggc
     1441 catgggtcgg gaggtgtcct caccccacgg gacttgcttc gttgcatacc ccattgggct
     1501 tctactcccc ttctctgtga ctggggcctg gtgtggcagg tcactttctg ttcagacata
     1561 cgcgtgtttt cagtgatcgc ctggtattaa tgttcacgtg gcttctggat ctttcgacct
     1621 ttagagaaac aacttcctag ttagcttcat cccatcatct gttgggcggc tcgttcctgg
     1681 tagtacccag tactccttac agacccttaa gacaggattg ttgggcctgg agtacggcac
     1741 aggctgttcg gcgtccaggg gaaggctgga agggattcgt aatgagattg gttgggtgtc
     1801 ccgtggatgt cgacccagtt ttttaaaatc gttagttgtg cagtcgtcat ggaactaaaa
     1861 cttactataa atagaaactc acctaactta gctttgtaat gcttgcatat tgccctcctc
     1921 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1981 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2041 tcttatcatg tctggatctg atcactgctt gagcctagga gatccgaacc agataagtga
     2101 aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc
     2161 cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc
     2221 ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt
     2281 ttaatcaaac atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct
     2341 gtcacagaat gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat
     2401 atcaacgctt atttgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     2461 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2521 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2581 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2641 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2701 ccctttgacg ttggagtcca cgttcttaat agtggactct tgttccaaac tggaacaaca
     2761 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2821 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2881 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2941 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3001 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3061 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3121 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3181 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3241 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3301 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3361 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3421 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3481 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3541 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3601 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3661 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3721 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3781 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3841 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3901 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3961 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4021 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4081 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4141 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4201 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4261 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4321 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4381 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4441 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4501 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4561 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4621 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4681 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4741 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4801 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     4861 gtatttcaca ccgcatagac cagccgcgta acctggcaaa atcggttacg gttgagtaat
     4921 aaatggatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat
     4981 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
     5041 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
     5101 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catggtaaag
     5161 actatattcg cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc
     5221 ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc
     5281 ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac
     5341 gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt
     5401 ggcaatgccc tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact
     5461 acgcggctgc tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg
     5521 gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga
     5581 gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag
     5641 agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat
     5701 acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
     5761 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
     5821 gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg
     5881 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
     5941 tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct
     6001 tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat
     6061 ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt caggcattgg
     6121 cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgcccttg cttcaggaga
     6181 tcggtagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc
     6241 gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccaggactct agctatagtt
     6301 ctagtggttg gctacgtacc cgtagtggct atggcagggc ttgccgcccc gacgttggct
     6361 gcgagccctg ggccttcacc cgaacttggg ggttggggtg gggaaaagga agaaacgcgg
     6421 gcgtattggt cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga
     6481 accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
     6541 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca
     6601 tctcccggta ccgcatgcta tgcatcagct gctagcacca tggctcgaga tcccgggtga
     6661 tcaagtcttc gtcgagtgat tgtaaataaa atgtaattta cagtatagta ttttaattaa
     6721 tatacaaatg atttgataat aattcttatt taactataat atattgtgtt gggttgaatt
     6781 aaaggtccgt atactccgga atattaatag atcatggaga taattaaaat gataaccatc
     6841 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
     6901 atattccgga ttattcatac cgtcccacca tcgggcgcgg atcccggtcc gaagcgcgcg
     6961 gaattcaaag gcctacgtcg acgagctcac tagtcgcggc cgctttcgaa tctagagcct
     7021 gcagtctcga caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg
     7081 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     7141 tgaatgcaat tgttgttgtt ccctggggta tattcagagc cccttcctcg cggcgttcat
     7201 atagtgtctc aggggtctgt cagccagcaa tccccaattc ctcattacat attcctcaca
     7261 atgcatgctg gctaaaatgg aaattgtgtt cagaaatagc cctgccctac catcatatac
     7321 taggagaaag gaggaaatta tgctctatta gaaaccctag gctatcttgc ataatgtttc
     7381 tcaatctgaa ataatttttt acagaatacc aatacgttaa gaagagctac ttccctgatc
     7441 tacctattat gggcagccca ttttatgaag ttagcatggc cttcctattc aaaccagaaa
     7501 tggacaaaaa gagaaggaag gcttttaggt cagtatggtt ataactgtag attctgtatt
     7561 cttcctcttt aatctatctc tcatattaac caaccaggag gttggaggcc ggaagtctta
     7621 gacgccagcg agagccgctc cttccccacg agtctatggc tttcgggtta cttaagccac
     7681 ggagaactct atggttccgg ggcgggccgg gaggtttgag tttgaagact ggcgggaaga
     7741 tgtccgcagc tgttgccagg ccagggttct cccgagaggg aggacgctgg gactgtggct
     7801 tgccctgatc ggccgagaag agtttgccat ggactacaaa gaccatgacg gtgattataa
     7861 agatcatgat atcgattaca aggatgacga tgacaagata acttcgtata atgtatgcta
     7921 tacgaagtta taagcttcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat
     7981 tttatttcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc
     8041 gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga
     8101 ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac
     8161 ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca
     8221 cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag
     8281 ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc
     8341 gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt
     8401 gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat
     8461 gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag
     8521 ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc
     8581 ggtcatgagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     8641 tagctcagag gccgaggcgg cctcggcctc tgcataaat
//
