LOCUS       sequence-42044-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PHF20 (3QII)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             329..346
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(399..417)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      413..460
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(584..603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     794..815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attccctctg aatattttgt ttaactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcga tggatcttct gaatttcaaa
      121 taaatgagca ggtccttgct tgctggtctg attgtcgttt ttacccggcc aaagtcactg
      181 ctgttaacaa ggatggtact tacactgtga aattttatga tggagtagtt cagactgtca
      241 aacatattca tgtcaaagct ttttccaaag atcagaatat tgtgggtaat gctaggcctt
      301 gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      361 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      421 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      481 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      541 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      601 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      781 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
      841 tattc
//
