LOCUS       sequence-420441-       17288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn7 with GmR and GUS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17288)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 17288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTSK65K_8G7
FEATURES             Location/Qualifiers
     source          1..17288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1204..1369
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             1789..3600
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        join(3772..3777,3778..3794,3795..3800)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3989..4522
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  10673..10897
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     misc_feature    11586..11878
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      12526..12979
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(13007..13029)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13115..13255
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13170..13189
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13270..13287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13441..14029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13521..13540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14208..15002)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14319..14338)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     14929..14948
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        15442..15657
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             15658..16461
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaatgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt ttttaaggat
     1261 tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa gctaatacga
     1321 aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggttt atctgcagca
     1381 ctcaacagat ctccgtctac ttgcgacacc tctcttaagg tagctggacc ggattcgggt
     1441 ctagcctata tcgtcaatcc gttgatctat gtcgggtgcg gagaaagagg taatgaaatg
     1501 ggcgatcgcc cacagcttac acagacctaa tcgtagagtt cctagtaata cttagtggct
     1561 cgttacctcg ttcttccagg ccagggggcc gtacgcgctc ggcgaacagt ataagcagat
     1621 tcgactcact agggataaca gggtaatctc tgggtatcga ctcaatctcg cgctattgtg
     1681 ctatatccgt ggacggttgc ggtcaattct gtcggaaaat caacggatac gaacaattac
     1741 tcgcgacttc ctcaatacgt gctgcaaaac tttagaagga gatataccat gttacgtcct
     1801 gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat
     1861 cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg cgttacaaga aagccgggca
     1921 attgctgtgc caggcagttt taacgatcag ttcgccgatg cagatattcg taattatgcg
     1981 ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc
     2041 gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt gggtcaataa tcaggaagtg
     2101 atggagcatc agggcggcta tacgccattt gaagccgatg tcacgccgta tgttattgcc
     2161 gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg
     2221 ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc
     2281 tttaactatg ccgggatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg
     2341 gacgatatca ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg
     2401 caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt
     2461 gcaactggac aaggcactag cgggactttg caagtggtga atccgcacct ctggcaaccg
     2521 ggtgaaggtt atctctatga actgtgcgtc acagccaaaa gccagacaga gtgtgatatc
     2581 tacccgcttc gcgtcggcat ccggtcagtg gcagtgaagg gcgaacagtt cctgattaac
     2641 cacaaaccgt tctactttac tggctttggt cgtcatgaag atgcggactt gcgtggcaaa
     2701 ggattcgata acgtgctgat ggtgcacgac cacgcattaa tggactggat tggggccaac
     2761 tcctaccgta cctcgcatta cccttacgct gaagagatgc tcgactgggc agatgaacat
     2821 ggcatcgtgg tgattgatga aactgctgct gtcggcttta acctctcttt aggcattggt
     2881 ttcgaagcgg gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa cggggaaact
     2941 cagcaagcgc acttacaggc gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc
     3001 gtggtgatgt ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc acgggaatat
     3061 ttcgcgccac tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc
     3121 aatgtaatgt tctgcgacgc tcacaccgat accatcagcg atctctttga tgtgctgtgc
     3181 ctgaaccgtt attacggatg gtatgtccaa agcggcgatt tggaaacggc agagaaggta
     3241 ctggaaaaag aacttctggc ctggcaggag aaactgcatc agccgattat catcaccgaa
     3301 tacggcgtgg atacgttagc cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag
     3361 tatcagtgtg catggctgga tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc
     3421 ggtgaacagg tatggaattt cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc
     3481 ggtaacaaga aagggatctt cactcgcgac cgcaaaccga agtcggcggc ttttctgctg
     3541 caaaaacgct ggactggcat gaacttcggt gaaaaaccgc agcagggagg caaacaatga
     3601 cgcgcgcgta actataacgg tcctaaggta gcgagcttac ttgtgctgtt gtgtaaggac
     3661 acgcttaacc cagagaattc cagcgaccta ttctacgact acgcgtccat ggctcgagga
     3721 gctcggtacc gccccactac ccgatttatc aagatttggc ggagagtccc attgacataa
     3781 gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     3841 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     3901 tgtttttttg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     3961 cgatgtttga tgttatggag cagcaacgat gttacgcagc agcaacgatg ttacgcagca
     4021 gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc gcacatgtag
     4081 gctcggccct gaccaagtca aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt
     4141 cggagacgta gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct
     4201 ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct
     4261 cgcggcttac gttctgccca agtttgagca gccgcgtagt gagatctata tctatgatct
     4321 cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa
     4381 gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga
     4441 tcccgcagtg gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat
     4501 cgacccaagt accgccacct aagtcgactg gcaaacagct attatgggta ttatgggtgt
     4561 gtatccagaa cgacggatct tgacgcaatt tggcgtggct ctcttaaggt agccctccga
     4621 ccaattgagg tgcgctctga ccgatcagtt tagtcactct tccccataaa ccatttccaa
     4681 gaatctttgt gcttcctcag ttatccgctc tttagataac gttgcacttc ttaataatcg
     4741 ccagcgccta agctcaacat cttcctgttt aagcttaata aaagcttggc taatccgtct
     4801 aatttgataa tcttccacac tctctgagta acgctttaag caaagcgcta ccaaaggcag
     4861 tttctgtaga ttttttgcaa gagaggttcc gttaggagtt tgctttaaca gccagctcga
     4921 tgtcgctctt gggtgatcaa ggctactatc tagacgcttt atgattctta atagttgtcg
     4981 tacagcaatt cgatctcttt ggttccaatc aactctaggg gcgggtgcca gacgctcttg
     5041 ttgatgctgt tgattccagt gaactagcca atccctgtca tgtcggtaaa gccaagcgta
     5101 tagcacccca ccctctaaag actgtcttgc cgccttaatt ccttggtatt tatgcactag
     5161 ttgctgccag tctttatgct taacagataa atcttcagag ttaggttgca cagactggct
     5221 aacaggtctt gtcgttatag agtgctcagt aagagcactt gcctgctgta gcgcttctat
     5281 aaccgttagt tttggcaata aggcttgcca cacaatacta tgctgtaaat aactaaaggc
     5341 ttttctatgc ttacggaata tacttttcag ccaacacgtg tccttgttct ctgccaactt
     5401 taaatccagt ttctctagtg cctcatcact aaaagtctgc ctcactctct ccgccaccaa
     5461 gtcatgacga atgtgcttgc ttttggttag ccctagatcc tgcgctaagc gctgataaaa
     5521 cagcgtccac tgctcaaggc ttggggaaag ctcttgcgct cgtggagcat ctaacagagg
     5581 ggctatataa gcagctagtg ctgttaattg agatagggag tctttggggt agtctgaaag
     5641 cagctcagta tgacccaaag cccaaaattg atgtcggtga tcatctacag ctctatcaaa
     5701 gaagactaaa gcaccgtgtt ttggacaata tggcaaagcg ggcaaatacc aatctcgttg
     5761 ccaaaaggct tccccatacc tatttagctg aagagcaacg caatcagggc agtatctaaa
     5821 gcggttatcg ctcttaactc tagaagcagc gactcctagc attaaatgca ccgcaccttg
     5881 cgcttggtac tccattaacc gaatagcttc gtctcggcgc tccttgccta caaacggagc
     5941 atataaaggg aataaggtat gctcataaat aagctgctga acagcgtaac gtcctgtttg
     6001 atgtagatgt cttgctatca cacctaaatg cgagggcaga cctaaggtag cgaccacctt
     6061 gcggttgcca tacacctcat ccaacagctg cttaggacta acaatccctt gataaacgcc
     6121 tgcccgtgca atagtgctat aaatcagctc attcgagtac ggaacaggaa agtttctcat
     6181 ggctaacctg cttgtttaaa taagctcgcc atatccacta ttaccccttt cccttttaac
     6241 cgttcatgca tggttttttc aggttggcgt tgtgaataga tataacgtaa atccgtatca
     6301 ggcaagctat cccagtcgct gggcttgact acctttatag ccgaaacctt tttactcttg
     6361 gagggctttt ctagttctga cactaccgtt tcgccttcca tcaaccactg caaaacaaga
     6421 ggcagtaact tttgtcttgt catcgttgga ttctggctaa acgctttttt aatagtggga
     6481 attaacaggc ttgaatcgta atcctctttc agcatcagat ataaatgacg ctgatcttcg
     6541 gtatctaact cttgaagggc tttttcttct ggtgtttgtt cttgtatcgc tgcgatatct
     6601 agctgaagtt ggattaaccg tttatcaatc tcgggaacga ctagatcaga ataacgagca
     6661 atgcgttctg ggatacccga gcgtaatgcc tctagcatgg ggtgcacagg ctttaactca
     6721 tcttgataca cttgccgcaa taaaccagcg gtaatacgct cattgcctaa agctagcgca
     6781 cggagctgag cgagtacaaa aagttttact acaatgtcca tcactccttg gcttagctca
     6841 taccacacat cacggacctc atccgataac agcgcatctt tgcgttgtaa aagctgtaat
     6901 tgccaaagat tatccgtaaa agcgatccac tcttgattgg gctttccacg ttgcgtttgt
     6961 tgtataggat cccagaatat agctccaaac cctgcccctc tacgtgcaga ccgcaaatca
     7021 gcctcaaaaa tctctcgtgc tttaggggta ccaatcaaca tcactggtac gccaataata
     7081 ttcaccatcg tcacaaaaaa gttcagcatc tcttgagatc cacccgaacg agagcggctt
     7141 aaatgctgaa tttcatcaat aaccaacaac cctaaagcat gtgcattggc tatttgcgac
     7201 atcaaagcca acatggtttc tataccatga cgttttaagc cataacgacg ctcatagttc
     7261 gagcccaagg ctcgatccaa cgctctgaaa aaattcaagc agatttcttt tagcgaacca
     7321 ttatgcgagc agtctatttt caaatacacc acctgctcta cattgagttc acgatggtaa
     7381 atcacctgag gatacgtggc tagaatacga tgaagagagg tcgtcttccc actaccagaa
     7441 caaccaatta acaataagct ttgtgccgta gatcgtgcct cctcaaagcg aaatgtctcc
     7501 aactctcccg tttgaacacg ctcataacca ttttgtaaat gcttttgtaa atctcctgtt
     7561 ttaggatttc tgcctacgta gccacctcga atcatgaccg aaatacgctc acttagtagc
     7621 aaatgcgtac ctaatggctg aaaatagtca tctggaatac gacaaatggt atgagctctg
     7681 ataacacggg acttttgcaa gtcagaggaa gtaagctgta aagaggattt cagtgatgca
     7741 gcactattca ctgactcttg taatggtggt aaggcctcga taaaaggatt atcacgataa
     7801 gcctctaccc ccgtatcacg ataaactgct tgaatccggg tagcactcat gactcatcct
     7861 tttctggtgg atcctgaaat aattcaggca cgtatgtggg taggctgtaa tcttcttgat
     7921 catccgcttc cactgcgttg aaaggaataa ctttagcctc atcacctgaa gagcttggct
     7981 tcaaatgctc cgcacgtttt ttacgctccg aggtcacggc ttctttttta ttagttttaa
     8041 tctgcttaat gcgtgttgat ttgggctcag tagtactggg cgttaactta ttcgctttct
     8101 gaatggtttg ctgaataaac gcctcaagct ccctgcgttt agttaactca tcctgcttcg
     8161 cattggcttt attgtgtttt tcttgtgctt gtatatccca aacctcccaa aatgagagac
     8221 ctttaaactg ccgactacgt tccgtcagat tacagcgcca aaatacacgg ctgccaactt
     8281 gcggaaacaa ataaatcgta tcaaccagca ctgggtcata agccgcttct aaatgttgag
     8341 gtctagctat atcagtgctc cgctgcaacc aaccctcacg cagaatctct gaccccgagt
     8401 aatacaaacc ccacaaatta acgccaaatg aagaaataga gacctttcgg cgaggcagta
     8461 acgctactcg caactgctct tgctccacag cccttaaact acctgtacga tgctgcatac
     8521 cccattgcca tagctggaca ggaatagacg gtaaatctgt aggaaaatca gcatctcgat
     8581 cgtatttatc catcaccaga tggttatttc tgaataagat cgtacgcaaa ataatttgtg
     8641 tgaactcaaa taccgacaga gatgcatcta acctatagtc tgtttcacca tggcttttga
     8701 tccgactgcc ctctacaatg ccaggtgcaa aggacttaaa ctcggcttgt agtgttctaa
     8761 aagtgctttc cactatgcct ttagcatcgc cacgtctagg tggagcactt tccactcgca
     8821 cattaaaact agaaactaag gcttcgacct gatgactcat taattcgcca cggtccgcta
     8881 gcaacacatc tggcaaacct acacacggcc agtctgagct actaatctca atatcatgct
     8941 gggcacaaat ggccgttttg tcagagcaag catttacaaa agcctgcatc gccaccacat
     9001 aagacggatt ttcaaagccg atataaaagc ccgtgatcat ccgactaaac acatcaatca
     9061 caatgtaaag cgttggtctt cctatgattt tttggcgatc atgatgatcc actaaataaa
     9121 tatcagcaat cgtggcatca atctcataac gactcccagg gcctaacgcc tgagaagtgg
     9181 ctgtactact taagggtcgt acgtcttttt tatatacccc tgctttaact ctagacttta
     9241 agcgctgagc tttagggtat tctcgatcat aaaaataacg aaactgacgt agtgttgggt
     9301 aatcctcttg gggaatgcga ggaaaatact gagcaaacaa gtccacaaat cgtctatagg
     9361 caacggtggt ctttgtacct ttttgattta acaggtgctt ttctatggtc aacctaaaaa
     9421 ggcgttcaat ctcgggcgtt accttggttc cttcaccctt accatattct ctggctcggc
     9481 caatctttgc tgttcctgtc gctgaacgtc tttcccctgg tgcaccgctg tttttgtagt
     9541 caggaattaa tgcattaggc gtttgaccac gctgccagta acggcgtaac aacttataaa
     9601 ctgtagcctt agtaacctta tgttcttgga ccacatgctc aacgagttcg cttctgactt
     9661 tagggtcgaa acgatcctta ctattaataa taggaagaat ttttcgataa tcctcctcgc
     9721 gcttctggaa gctgacagaa tctacagacg gctcttctaa atcaagatgt acataagggt
     9781 catcagttct aactaagcgg ccttcatcta aatactgcat tagcaacaac tcagccctag
     9841 cttgtggaac tcctttatca gcgcttattt gcatccagac aacttggcca tcctctatag
     9901 ccaaaatgcg atacggatca ttatcaaata gcacaacctc attaatttgc cacatagcgc
     9961 aactcctcca tattcactac ttggctaata cagagatctg cacacttatt tgccctgaaa
    10021 gacttataaa tattgaactt aataaaacca tttgcggtta aggctcgtat ctcactcaat
    10081 gttttgccta actccaaatc ataagcaata tcaacctgct tacagacatt gataatgttt
    10141 tcatctcctt tttcttgcag gatatgggcc aatggggata gttgtgctaa aagctccgca
    10201 gaaacttctt ctgttttcac tgaataaagc cattcaatat tttcttttac tacgggattt
    10261 atttctttat cagtaaaaat gaaccaagga atttgctttt gctgccaata gcgacgctct
    10321 agttctagtt tttctaaggt acgctcgtct tgtaaggctg ctgcaggttt gacttgaata
    10381 gcaaactgct caaaaggacc atctttgcag tccactaaaa aatcagtaga cataacctga
    10441 tctacaccac gaataacagg atgcttaata ccactatcta ttgcaatctg cctggtatca
    10501 ctaggtaata aggggaactg ctcgcgtata tctagcacgc tgctctccca ctcaagactg
    10561 agaaaaacag caagctctaa gtcagatagc aaatgatgga ctcgtcccgt cttatgagaa
    10621 taaatacggt gggaacgacc tgaagaagga acttcttgta ctgttagcca tggaatatag
    10681 tctttaccat gcccttggcc acgcccctct ttaatacgac gggcaatttg cacttcagaa
    10741 aatgaagagt ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg
    10801 actgatttca gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc
    10861 tattttgttc agtttaagac tttattgtcc gcccacaccc gcttacgcag ggcatccatt
    10921 tattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg
    10981 atcagcgcga catggtaagc cagcagctgc agcggaacgg tgtagaagat cggtgcaatc
    11041 acctcttcca catgcggcat ctcgacccgg gactagttgg tgtggaagtc ctaatatcgg
    11101 tgatcaatat agtggttgac atgctggcta gtcaacattg agccttttga tcatgcaaat
    11161 atattacggt attttacaat caaatatcaa acttaactat tgactttata acttatttag
    11221 gtggtaacat tcttataaaa aagaaaaaaa ttactgcaaa acagtactag cttttaactt
    11281 gtatcctagg ttatctatgc tgtctcacca tagagaatat tacctatttc agaatgtatg
    11341 tccatgattc gccgggtaaa tacatataat acacaaatct ggcttaataa agtctataat
    11401 atatctcata aagaagtgct aaattggcta gtgctatata tttttaagaa aatttctttt
    11461 gactaagtcc atatcgactt tgtaaaagtt cacattagca tacatatatt acacgagcca
    11521 gaaatagtaa cttttgccta aatcacaaat tgcaaaattt aattgcttgc aaaaggtcac
    11581 atgcttataa tcaacttttt taaaaattta aaatactttt ttatttttta tttttaaaca
    11641 taaatgaaat aatttattta ttgtttatga ttaccgaaac ataaaacctg ctcaagaaaa
    11701 agaaactgtt ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa tgccgaggct
    11761 ttcatagctt aaactcttta cagaaaatag gcattataga tcagttcgag ttttcttatt
    11821 cttccttccg gttttatcgt cacagtttta cagtaaataa gtatcacctc ttagagttaa
    11881 cctatactaa aatttatgcc agttaactat gagataagca agtatcatct catttcatta
    11941 cctgaagtcg agtaaacaga aaatccaatt gttgatgaac ctcaatgact tagaactatc
    12001 tatcggcaga tcatataaag aggatttagg tactcgagaa cgctccgtac atgcagctaa
    12061 acctccaagc atctctaact tgggtagtgc tttctccacc attttctgtg cttgctcctt
    12121 cgtggcaagt ccagcccata atgcccagaa tgtagttgcg gattcgtatg acgttctgtg
    12181 cttgattttt gtgttgtagt caaagaaaag gtccggttaa acggatctcg cattgatgag
    12241 gcaacgctaa ttatcaacat atagattgtt atctatctgc atgaacacga aatctttact
    12301 tgacgacttg aggctgatgg tgtttatgca aagaaaccac tgtgtttaat atgtgtcact
    12361 gtttgatatt actgtcagcg tagaagataa tagtaaaagc ggttaataag tgtatttgag
    12421 ataagtgtga taaagttttt acagcgaaaa gacgataaat acaagaaaat gattacgagg
    12481 atacggagag aggtatgtac atgtgtattt atatactaag ctgccggcgg ttgtttgcaa
    12541 gaccgagaaa aggctagcaa gaatcgggtc attgtagcgt atgcgcctgt gaacattctc
    12601 ttcaacaagt ttgattccat tgcggtgaaa tggtaaaagt caaccccctg cgatgtatat
    12661 tttcctgtac aatcaatcaa aaagccaaat gatttagcat tatctttaca tcttgttatt
    12721 ttacagattt tatgtttaga tcttttatgc ttgcttttca aaaggcctgc aggcaagtgc
    12781 acaaacaata cttaaataaa tactactcag taataaccta tttcttagca tttttgacga
    12841 aatttgctat tttgttagag tcttttacac catttgtctc cacacctccg cttacatcaa
    12901 caccaataac gccatttaat ctaagcgcat caccaacatt ttctggcgtc agtccaccag
    12961 ctaacataaa atgttgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    13021 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    13081 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
    13141 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    13201 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
    13261 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    13321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    13381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    13441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    13501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    13561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    13621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    13681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    13741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    13801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    13861 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    13921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    13981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    14041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    14101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    14161 atcaatctaa agtatatatg agtaaacttg gtctgacagg agctcgctca gaagaactcg
    14221 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
    14281 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
    14341 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
    14401 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
    14461 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
    14521 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
    14581 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
    14641 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
    14701 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
    14761 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
    14821 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
    14881 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
    14941 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
    15001 atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc
    15061 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
    15121 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
    15181 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
    15241 atagcccagt agctgacatt catccgggcg atcgatggaa gccggcggca cctcgctaac
    15301 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa
    15361 accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc
    15421 atcggggggg gggggggggg tttcaattca tcattttttt tttattcttt tttttgattt
    15481 cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag
    15541 gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc
    15601 ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg
    15661 tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta
    15721 tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc
    15781 aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact aaaaacacat
    15841 gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa ggcattatcc
    15901 gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg taatacagtc
    15961 aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat tacgaatgca
    16021 cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga agaagtaaca
    16081 aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc cctatctact
    16141 ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt tgttatcggc
    16201 tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt gattatgaca
    16261 cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag aaccgtggat
    16321 gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt tgcaaaggga
    16381 agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga
    16441 agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca
    16501 caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg tcgtaatgat
    16561 ttttataatg acgaaaaaaa aaaaattgga aagaaaaccc cccccccccg cagcgttggg
    16621 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
    16681 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
    16741 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
    16801 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
    16861 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
    16921 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
    16981 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
    17041 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
    17101 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
    17161 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
    17221 gaccacgctg atgagcttta ccgctgggcc attctcatga agaatatctt gaatttattg
    17281 tcatatta
//
