LOCUS       sequence-420455-       13310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of untagged 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13310)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 13310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pJRA99
FEATURES             Location/Qualifiers
     source          1..13310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    2980..3104
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3129..3134,3135..3152,3153..3159)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3213..3872
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3279..3298)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4214..4519
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4398..4417
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4560..4684)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4743..5450
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5479..5496)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5479..5495)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5488..5510)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5698..5722
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7023..7652
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8081..9154
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9220..9414
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9650..9672)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9758..9898
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9813..9832
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9913..9930)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10084..10672)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10164..10183)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12151..12942
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gaagcaccca ccctaagcca cattttaatc cttctgttgg ccatgcccca
     1381 tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg tctgtcccga
     1441 cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc
     1501 tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac
     1561 cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt
     1621 cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga
     1681 tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg
     1741 tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg
     1801 agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag
     1861 aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata
     1921 caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa
     1981 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     2041 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     2101 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     2161 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     2221 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     2281 gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg
     2341 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     2401 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     2461 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     2521 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     2581 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     2641 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     2701 tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag
     2761 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     2821 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     2881 ctagtttgtg cgatcgaatt tgtcctcgag tccggactca gatcacgagc tcaagcttca
     2941 aattctgcag tcgacggtac cgcgggcccg ggatcatcaa caagtttgta caaaaaagct
     3001 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     3061 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
     3121 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     3181 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     3241 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     3301 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     3361 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     3421 atccggagtt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     3481 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     3541 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     3601 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     3661 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     3721 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     3781 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     3841 agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca
     3901 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     3961 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     4021 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     4081 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     4141 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     4201 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     4261 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     4321 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     4381 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     4441 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     4501 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     4561 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     4621 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     4681 tggttgatga tcctaggtga gtctagagag ttaattaaga cccgggacta gtccctagag
     4741 tcctgcttta atgagatatg cgagacgcct atgatcgcat gatatttgct ttcaattctg
     4801 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
     4861 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
     4921 aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg
     4981 tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt
     5041 ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga
     5101 ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg
     5161 agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt
     5221 gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca
     5281 acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt
     5341 ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc
     5401 gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag gacctgcatg
     5461 caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5521 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5581 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag
     5641 cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
     5701 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     5761 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     5821 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     5881 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     5941 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     6001 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     6061 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     6121 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     6181 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
     6241 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
     6301 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
     6361 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
     6421 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
     6481 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
     6541 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
     6601 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
     6661 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
     6721 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
     6781 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg
     6841 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
     6901 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     6961 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     7021 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     7081 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     7141 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     7201 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     7261 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     7321 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
     7381 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
     7441 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
     7501 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
     7561 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
     7621 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
     7681 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
     7741 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
     7801 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
     7861 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
     7921 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
     7981 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc
     8041 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc
     8101 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc
     8161 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
     8221 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
     8281 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
     8341 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
     8401 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
     8461 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca
     8521 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
     8581 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
     8641 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
     8701 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
     8761 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
     8821 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
     8881 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
     8941 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
     9001 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
     9061 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
     9121 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
     9181 tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg
     9241 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat
     9301 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc
     9361 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
     9421 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct
     9481 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct
     9541 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg
     9601 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     9661 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     9721 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     9781 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     9841 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     9901 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9961 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    10021 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10081 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10141 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10201 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10261 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10321 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10381 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10441 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10501 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    10561 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10621 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10681 tttgatcttt tctacggggt cctgatgaat cccctaatga ttttggtaaa aatcattaag
    10741 ttaaggtgga tacacatctt gtcatatgat caaatggttt cgcgaaaaat caataatcag
    10801 acaacaagat gtgcgaactc gatattttac acgactctct ttaccaattc tgccccgaat
    10861 tacacttaaa acgactcaac agcttaacgt tggcttgcca cgcattactt gactgtaaaa
    10921 ctctcactct taccgaactt ggccgtaacc tgccaaccaa agcgagaaca aaacataaca
    10981 tcaaacgaat cgaccgattg ttaggtaatc gtcacctcca caaagagcga ctcgctgtat
    11041 accgttggca tgctagcttt atctgttcgg gcaatacgat gcccattgta cttgttgact
    11101 ggtctgatat tcgtgagcaa aaacgactta tggtattgcg agcttcagtc gcactacacg
    11161 gtcgttctgt tactctttat gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag
    11221 ctcatgacca atttctagcc gaccttgcga gcattctacc gagtaacacc acaccgctca
    11281 ttgtcagtga tgctggcttt aaagtgccat ggtataaatc cgttgagaag ctgggttggt
    11341 actggttaag tcgagtaaga ggaaaagtac aatatgcaga cctaggagcg gaaaactgga
    11401 aacctatcag caacttacat gatatgtcat ctagtcactc aaagacttta ggctataaga
    11461 ggctgactaa aagcaatcca atctcatgcc aaattctatt gtataaatct cgctctaaag
    11521 gccgaaaaaa tcagcgctcg acacggactc attgtcacca cccgtcacct aaaatctact
    11581 cagcgtcggc aaaggagcca tgggttctag caactaactt acctgttgaa attcgaacac
    11641 ccaaacaact tgttaatatc tattcgaagc gaatgcagat tgaagaaacc ttccgagact
    11701 tgaaaagtcc tgcctacgga ctaggcctac gccatagccg aacgagcagc tcagagcgtt
    11761 ttgatatcat gctgctaatc gccctgatgc ttcaactaac atgttggctt gcgggcgttc
    11821 atgctcagaa acaaggttgg gacaagcact tccaggctaa cacagtcaga aatcgaaacg
    11881 tactctcaac agttcgctta ggcatggaag ttttgcggca ttctggctac acaataacaa
    11941 gggaagactt actcgtggct gcaaccctac tagctcaaaa tttattcaca catggttacg
    12001 ctttggggaa attatgaggg gatctctcag tacggggtct gacgctcagt ggaacgaaaa
    12061 ctcacgttaa gggattttgg tcatgcaatg ttatggagca gcaacgatgt tacgcagcag
    12121 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
    12181 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
    12241 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
    12301 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
    12361 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
    12421 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
    12481 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
    12541 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
    12601 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
    12661 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
    12721 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
    12781 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
    12841 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
    12901 cactacgtga aaggcgagat caccaaggta gtcggcaaat aataacaaga cgaactccaa
    12961 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    13021 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
    13081 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
    13141 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
    13201 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    13261 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
//
