LOCUS       sequence-420456-       14027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            mVenus fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14027)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 14027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pJRA101
FEATURES             Location/Qualifiers
     source          1..14027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             join(2911..2913,2914..2916,2917..3627)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2956..2977)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3217..3236)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3564..3585
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3697..3821
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3846..3851,3852..3869,3870..3876)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3930..4589
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3996..4015)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4931..5236
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5115..5134
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5277..5401)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5460..6167
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6196..6213)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6196..6212)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6205..6227)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6415..6439
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7740..8369
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8798..9871
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9937..10131
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10367..10389)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10475..10615
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10530..10549
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10630..10647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10801..11389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10881..10900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12868..13659
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gaagcaccca ccctaagcca cattttaatc cttctgttgg ccatgcccca
     1381 tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg tctgtcccga
     1441 cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc
     1501 tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac
     1561 cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt
     1621 cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga
     1681 tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg
     1741 tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg
     1801 agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag
     1861 aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata
     1921 caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa
     1981 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     2041 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     2101 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     2161 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     2221 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     2281 gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg
     2341 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     2401 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     2461 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     2521 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     2581 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     2641 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     2701 tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag
     2761 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     2821 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     2881 ctagtttgtg cgatcgaatt tgtcctcgag atggtgagca agggcgagga gctgttcacc
     2941 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3001 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     3061 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag
     3121 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     3181 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     3241 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     3301 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     3361 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     3421 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     3481 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa actgagcaaa
     3541 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     3601 actctcggca tggacgagct gtacaagtcc ggactcagat cacgagctca agcttcaaat
     3661 tctgcagtcg acggtaccgc gggcccggga tcatcaacaa gtttgtacaa aaaagctgaa
     3721 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     3781 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
     3841 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
     3901 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     3961 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     4021 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     4081 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     4141 cggagttccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     4201 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     4261 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     4321 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     4381 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     4441 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     4501 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     4561 actgcgatga gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat
     4621 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     4681 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     4741 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     4801 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     4861 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     4921 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     4981 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     5041 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     5101 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     5161 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     5221 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
     5281 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     5341 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
     5401 ttgatgatcc taggtgagtc tagagagtta attaagaccc gggactagtc cctagagtcc
     5461 tgctttaatg agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg
     5521 tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca
     5581 ttctaatgaa tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat
     5641 ttactgattg taccctacta cttatatgta caatattaaa atgaaaacaa tatattgtgc
     5701 tgaataggtt tatagcgaca tctatgatag agcgccacaa taacaaacaa ttgcgtttta
     5761 ttattacaaa tccaatttta aaaaaagcgg cagaaccggt caaacctaaa agactgatta
     5821 cataaatctt attcaaattt caaaagtgcc ccaggggcta gtatctacga cacaccgagc
     5881 ggcgaactaa taacgctcac tgaagggaac tccggttccc cgccggcgcg catgggtgag
     5941 attccttgaa gttgagtatt ggccgtccgc tctaccgaaa gttacgggca ccattcaacc
     6001 cggtccagca cggcggccgg gtaaccgact tgctgccccg agaattatgc agcatttttt
     6061 tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt
     6121 gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc tcagcaggac ctgcatgcag
     6181 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6241 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6301 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     6361 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     6421 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     6481 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     6541 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     6601 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     6661 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     6721 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     6781 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     6841 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     6901 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     6961 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     7021 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     7081 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     7141 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     7201 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     7261 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     7321 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     7381 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     7441 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     7501 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     7561 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     7621 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     7681 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     7741 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     7801 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     7861 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     7921 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     7981 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     8041 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     8101 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     8161 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     8221 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     8281 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     8341 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     8401 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     8461 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     8521 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     8581 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     8641 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     8701 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
     8761 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     8821 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     8881 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     8941 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     9001 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     9061 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     9121 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     9181 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     9241 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     9301 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     9361 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     9421 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     9481 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     9541 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     9601 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     9661 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     9721 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     9781 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     9841 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     9901 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     9961 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
    10021 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
    10081 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
    10141 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
    10201 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
    10261 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
    10321 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10381 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10441 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
    10501 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10561 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
    10621 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11221 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    11281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11401 gatcttttct acggggtcct gatgaatccc ctaatgattt tggtaaaaat cattaagtta
    11461 aggtggatac acatcttgtc atatgatcaa atggtttcgc gaaaaatcaa taatcagaca
    11521 acaagatgtg cgaactcgat attttacacg actctcttta ccaattctgc cccgaattac
    11581 acttaaaacg actcaacagc ttaacgttgg cttgccacgc attacttgac tgtaaaactc
    11641 tcactcttac cgaacttggc cgtaacctgc caaccaaagc gagaacaaaa cataacatca
    11701 aacgaatcga ccgattgtta ggtaatcgtc acctccacaa agagcgactc gctgtatacc
    11761 gttggcatgc tagctttatc tgttcgggca atacgatgcc cattgtactt gttgactggt
    11821 ctgatattcg tgagcaaaaa cgacttatgg tattgcgagc ttcagtcgca ctacacggtc
    11881 gttctgttac tctttatgag aaagcgttcc cgctttcaga gcaatgttca aagaaagctc
    11941 atgaccaatt tctagccgac cttgcgagca ttctaccgag taacaccaca ccgctcattg
    12001 tcagtgatgc tggctttaaa gtgccatggt ataaatccgt tgagaagctg ggttggtact
    12061 ggttaagtcg agtaagagga aaagtacaat atgcagacct aggagcggaa aactggaaac
    12121 ctatcagcaa cttacatgat atgtcatcta gtcactcaaa gactttaggc tataagaggc
    12181 tgactaaaag caatccaatc tcatgccaaa ttctattgta taaatctcgc tctaaaggcc
    12241 gaaaaaatca gcgctcgaca cggactcatt gtcaccaccc gtcacctaaa atctactcag
    12301 cgtcggcaaa ggagccatgg gttctagcaa ctaacttacc tgttgaaatt cgaacaccca
    12361 aacaacttgt taatatctat tcgaagcgaa tgcagattga agaaaccttc cgagacttga
    12421 aaagtcctgc ctacggacta ggcctacgcc atagccgaac gagcagctca gagcgttttg
    12481 atatcatgct gctaatcgcc ctgatgcttc aactaacatg ttggcttgcg ggcgttcatg
    12541 ctcagaaaca aggttgggac aagcacttcc aggctaacac agtcagaaat cgaaacgtac
    12601 tctcaacagt tcgcttaggc atggaagttt tgcggcattc tggctacaca ataacaaggg
    12661 aagacttact cgtggctgca accctactag ctcaaaattt attcacacat ggttacgctt
    12721 tggggaaatt atgaggggat ctctcagtac ggggtctgac gctcagtgga acgaaaactc
    12781 acgttaaggg attttggtca tgcaatgtta tggagcagca acgatgttac gcagcagggc
    12841 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
    12901 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
    12961 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
    13021 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
    13081 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
    13141 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
    13201 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
    13261 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
    13321 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
    13381 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
    13441 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
    13501 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
    13561 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
    13621 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat aacaagacga actccaattc
    13681 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    13741 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
    13801 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
    13861 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
    13921 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
    13981 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctgg
//
