LOCUS       sequence-420457-       13856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            SNAP-tag fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13856)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 13856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pJRA103
FEATURES             Location/Qualifiers
     source          1..13856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2911..3456
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     protein_bind    3526..3650
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3675..3680,3681..3698,3699..3705)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3759..4418
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3825..3844)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4760..5065
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4944..4963
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5106..5230)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5289..5996
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6025..6042)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6025..6041)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6034..6056)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6244..6268
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7569..8198
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8627..9700
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9766..9960
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10196..10218)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10304..10444
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10359..10378
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10459..10476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10630..11218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10710..10729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12697..13488
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gaagcaccca ccctaagcca cattttaatc cttctgttgg ccatgcccca
     1381 tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg tctgtcccga
     1441 cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc
     1501 tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac
     1561 cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt
     1621 cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga
     1681 tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg
     1741 tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg
     1801 agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag
     1861 aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata
     1921 caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa
     1981 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     2041 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     2101 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     2161 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     2221 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     2281 gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg
     2341 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     2401 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     2461 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     2521 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     2581 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     2641 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     2701 tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag
     2761 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     2821 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     2881 ctagtttgtg cgatcgaatt tgtcctcgag atggacaaag actgcgaaat gaagcgcacc
     2941 accctggata gccctctggg caagctggaa ctgtctgggt gcgaacaggg cctgcaccgt
     3001 atcatcttcc tgggcaaagg aacatctgcc gccgacgccg tggaagtgcc tgccccagcc
     3061 gccgtgctgg gcggaccaga gccactgatg caggccaccg cctggctcaa cgcctacttt
     3121 caccagcctg aggccatcga ggagttccct gtgccagccc tgcaccaccc agtgttccag
     3181 caggagagct ttacccgcca ggtgctgtgg aaactgctga aagtggtgaa gttcggagag
     3241 gtcatcagct acagccacct ggccgccctg gccggcaatc ccgccgccac cgccgccgtg
     3301 aaaaccgccc tgagcggaaa tcccgtgccc attctgatcc cctgccaccg ggtggtgcag
     3361 ggcgacctgg acgtgggggg ctacgagggc gggctcgccg tgaaagagtg gctgctggcc
     3421 cacgagggcc acagactggg caagcctggg ctgggttccg gactcagatc acgagctcaa
     3481 gcttcaaatt ctgcagtcga cggtaccgcg ggcccgggat catcaacaag tttgtacaaa
     3541 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     3601 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
     3661 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     3721 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     3781 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     3841 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     3901 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     3961 atgctcatcc ggagttccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     4021 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     4081 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     4141 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     4201 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     4261 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     4321 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     4381 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa
     4441 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     4501 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
     4561 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     4621 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     4681 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     4741 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     4801 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     4861 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
     4921 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     4981 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     5041 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     5101 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     5161 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     5221 acaaagtggt tgatgatcct aggtgagtct agagagttaa ttaagacccg ggactagtcc
     5281 ctagagtcct gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca
     5341 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt
     5401 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct
     5461 ccgttcaatt tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat
     5521 atattgtgct gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat
     5581 tgcgttttat tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa
     5641 gactgattac ataaatctta ttcaaatttc aaaagtgccc caggggctag tatctacgac
     5701 acaccgagcg gcgaactaat aacgctcact gaagggaact ccggttcccc gccggcgcgc
     5761 atgggtgaga ttccttgaag ttgagtattg gccgtccgct ctaccgaaag ttacgggcac
     5821 cattcaaccc ggtccagcac ggcggccggg taaccgactt gctgccccga gaattatgca
     5881 gcattttttt ggtgtatgtg ggccccaaat gaagtgcagg tcaaaccttg acagtgacga
     5941 caaatcgttg ggcgggtcca gggcgaattt tgcgacaaca tgtcgaggct cagcaggacc
     6001 tgcatgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6061 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6121 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6181 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
     6241 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     6301 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     6361 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     6421 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     6481 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     6541 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     6601 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     6661 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     6721 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     6781 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     6841 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     6901 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     6961 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     7021 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     7081 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     7141 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     7201 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     7261 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     7321 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     7381 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     7441 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     7501 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     7561 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     7621 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     7681 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     7741 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     7801 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     7861 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     7921 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     7981 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     8041 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     8101 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     8161 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     8221 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     8281 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     8341 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     8401 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     8461 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     8521 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     8581 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     8641 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     8701 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     8761 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     8821 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     8881 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     8941 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     9001 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     9061 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     9121 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     9181 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     9241 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     9301 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     9361 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     9421 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     9481 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     9541 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     9601 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     9661 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     9721 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
     9781 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     9841 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     9901 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     9961 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
    10021 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
    10081 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
    10141 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
    10201 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    10261 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
    10321 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
    10381 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10441 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10501 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10801 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11041 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    11101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11221 agatcctttg atcttttcta cggggtcctg atgaatcccc taatgatttt ggtaaaaatc
    11281 attaagttaa ggtggataca catcttgtca tatgatcaaa tggtttcgcg aaaaatcaat
    11341 aatcagacaa caagatgtgc gaactcgata ttttacacga ctctctttac caattctgcc
    11401 ccgaattaca cttaaaacga ctcaacagct taacgttggc ttgccacgca ttacttgact
    11461 gtaaaactct cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac
    11521 ataacatcaa acgaatcgac cgattgttag gtaatcgtca cctccacaaa gagcgactcg
    11581 ctgtataccg ttggcatgct agctttatct gttcgggcaa tacgatgccc attgtacttg
    11641 ttgactggtc tgatattcgt gagcaaaaac gacttatggt attgcgagct tcagtcgcac
    11701 tacacggtcg ttctgttact ctttatgaga aagcgttccc gctttcagag caatgttcaa
    11761 agaaagctca tgaccaattt ctagccgacc ttgcgagcat tctaccgagt aacaccacac
    11821 cgctcattgt cagtgatgct ggctttaaag tgccatggta taaatccgtt gagaagctgg
    11881 gttggtactg gttaagtcga gtaagaggaa aagtacaata tgcagaccta ggagcggaaa
    11941 actggaaacc tatcagcaac ttacatgata tgtcatctag tcactcaaag actttaggct
    12001 ataagaggct gactaaaagc aatccaatct catgccaaat tctattgtat aaatctcgct
    12061 ctaaaggccg aaaaaatcag cgctcgacac ggactcattg tcaccacccg tcacctaaaa
    12121 tctactcagc gtcggcaaag gagccatggg ttctagcaac taacttacct gttgaaattc
    12181 gaacacccaa acaacttgtt aatatctatt cgaagcgaat gcagattgaa gaaaccttcc
    12241 gagacttgaa aagtcctgcc tacggactag gcctacgcca tagccgaacg agcagctcag
    12301 agcgttttga tatcatgctg ctaatcgccc tgatgcttca actaacatgt tggcttgcgg
    12361 gcgttcatgc tcagaaacaa ggttgggaca agcacttcca ggctaacaca gtcagaaatc
    12421 gaaacgtact ctcaacagtt cgcttaggca tggaagtttt gcggcattct ggctacacaa
    12481 taacaaggga agacttactc gtggctgcaa ccctactagc tcaaaattta ttcacacatg
    12541 gttacgcttt ggggaaatta tgaggggatc tctcagtacg gggtctgacg ctcagtggaa
    12601 cgaaaactca cgttaaggga ttttggtcat gcaatgttat ggagcagcaa cgatgttacg
    12661 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
    12721 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
    12781 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
    12841 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
    12901 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
    12961 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
    13021 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
    13081 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
    13141 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
    13201 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
    13261 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
    13321 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
    13381 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
    13441 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataata acaagacgaa
    13501 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
    13561 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
    13621 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
    13681 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
    13741 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
    13801 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctgg
//
