LOCUS       sequence-420458-       14201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            HaloTag fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14201)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 14201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pJRA104
FEATURES             Location/Qualifiers
     source          1..14201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2911..3801
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     protein_bind    3871..3995
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4020..4025,4026..4043,4044..4050)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4104..4763
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4170..4189)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5105..5410
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5289..5308
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5451..5575)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5634..6341
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6370..6387)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6370..6386)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6379..6401)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6589..6613
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7914..8543
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8972..10045
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10111..10305
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10541..10563)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10649..10789
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10704..10723
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10804..10821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10975..11563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11055..11074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13042..13833
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gaagcaccca ccctaagcca cattttaatc cttctgttgg ccatgcccca
     1381 tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg tctgtcccga
     1441 cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc
     1501 tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac
     1561 cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt
     1621 cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga
     1681 tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg
     1741 tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg
     1801 agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag
     1861 aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata
     1921 caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa
     1981 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     2041 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     2101 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     2161 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     2221 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     2281 gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg
     2341 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     2401 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     2461 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     2521 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     2581 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     2641 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     2701 tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag
     2761 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     2821 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     2881 ctagtttgtg cgatcgaatt tgtcctcgag atggcagaaa tcggtactgg ctttccattc
     2941 gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc
     3001 gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac
     3061 atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc
     3121 aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc
     3181 ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct
     3241 ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag
     3301 ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag
     3361 gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag
     3421 ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc
     3481 gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca
     3541 atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac
     3601 cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc
     3661 gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt
     3721 ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg
     3781 tcgacgcttg agatttccgg ctccggactc agatcacgag ctcaagcttc aaattctgca
     3841 gtcgacggta ccgcgggccc gggatcatca acaagtttgt acaaaaaagc tgaacgagaa
     3901 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     3961 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     4021 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     4081 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     4141 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     4201 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     4261 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggagt
     4321 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     4381 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     4441 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     4501 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     4561 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     4621 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     4681 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     4741 atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt
     4801 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     4861 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     4921 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     4981 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     5041 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     5101 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     5161 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     5221 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     5281 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     5341 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     5401 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     5461 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     5521 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgatg
     5581 atcctaggtg agtctagaga gttaattaag acccgggact agtccctaga gtcctgcttt
     5641 aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg
     5701 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa
     5761 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg
     5821 attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata
     5881 ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta
     5941 caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa
     6001 tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa
     6061 ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct
     6121 tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc
     6181 agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt
     6241 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
     6301 gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcat gcaggcatgc
     6361 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6421 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6481 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     6541 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     6601 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     6661 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     6721 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     6781 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     6841 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     6901 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     6961 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     7021 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     7081 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     7141 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     7201 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     7261 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     7321 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     7381 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     7441 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     7501 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     7561 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     7621 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     7681 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     7741 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     7801 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     7861 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     7921 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     7981 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     8041 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     8101 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     8161 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     8221 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     8281 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     8341 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     8401 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     8461 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     8521 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     8581 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     8641 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     8701 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     8761 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     8821 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     8881 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
     8941 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     9001 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     9061 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     9121 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     9181 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     9241 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     9301 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     9361 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     9421 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     9481 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     9541 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     9601 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     9661 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     9721 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     9781 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     9841 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     9901 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     9961 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
    10021 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
    10081 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
    10141 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
    10201 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
    10261 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
    10321 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
    10381 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
    10441 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
    10501 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    10561 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    10621 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
    10681 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10741 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
    10801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11161 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11401 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11581 ttctacgggg tcctgatgaa tcccctaatg attttggtaa aaatcattaa gttaaggtgg
    11641 atacacatct tgtcatatga tcaaatggtt tcgcgaaaaa tcaataatca gacaacaaga
    11701 tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa
    11761 aacgactcaa cagcttaacg ttggcttgcc acgcattact tgactgtaaa actctcactc
    11821 ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa
    11881 tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc
    11941 atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata
    12001 ttcgtgagca aaaacgactt atggtattgc gagcttcagt cgcactacac ggtcgttctg
    12061 ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc
    12121 aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg
    12181 atgctggctt taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa
    12241 gtcgagtaag aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca
    12301 gcaacttaca tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta
    12361 aaagcaatcc aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa
    12421 atcagcgctc gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg
    12481 caaaggagcc atgggttcta gcaactaact tacctgttga aattcgaaca cccaaacaac
    12541 ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc
    12601 ctgcctacgg actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca
    12661 tgctgctaat cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga
    12721 aacaaggttg ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa
    12781 cagttcgctt aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact
    12841 tactcgtggc tgcaacccta ctagctcaaa atttattcac acatggttac gctttgggga
    12901 aattatgagg ggatctctca gtacggggtc tgacgctcag tggaacgaaa actcacgtta
    12961 agggattttg gtcatgcaat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
    13021 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
    13081 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
    13141 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
    13201 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
    13261 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
    13321 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
    13381 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
    13441 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
    13501 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
    13561 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
    13621 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
    13681 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
    13741 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
    13801 aaaggcgaga tcaccaaggt agtcggcaaa taataacaag acgaactcca attcactgtt
    13861 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    13921 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
    13981 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
    14041 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
    14101 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
    14161 tttgtgccga gctgccggtc ggggagctgt tggctggctg g
//
