LOCUS       sequence-420459-       14000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            Dendra2 fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14000)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 14000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pJRA105
FEATURES             Location/Qualifiers
     source          1..14000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2911..3600
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     protein_bind    3670..3794
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3819..3824,3825..3842,3843..3849)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3903..4562
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3969..3988)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4904..5209
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5088..5107
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5250..5374)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5433..6140
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6169..6186)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6169..6185)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6178..6200)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6388..6412
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7713..8342
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8771..9844
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9910..10104
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10340..10362)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10448..10588
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10503..10522
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10603..10620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10774..11362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10854..10873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12841..13632
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gaagcaccca ccctaagcca cattttaatc cttctgttgg ccatgcccca
     1381 tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg tctgtcccga
     1441 cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc
     1501 tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac
     1561 cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt
     1621 cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga
     1681 tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg
     1741 tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg
     1801 agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag
     1861 aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata
     1921 caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa
     1981 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     2041 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     2101 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     2161 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     2221 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     2281 gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg
     2341 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     2401 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     2461 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     2521 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     2581 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     2641 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     2701 tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag
     2761 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     2821 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     2881 ctagtttgtg cgatcgaatt tgtcctcgag atgaacaccc cgggaattaa cctgatcaag
     2941 gaggacatgc gcgtgaaggt gcacatggag ggcaacgtga acggccacgc cttcgtgatc
     3001 gagggcgagg gcaagggcaa gccctacgag ggcacccaga ccgccaacct gaccgtgaag
     3061 gagggcgccc ccctgccctt cagctacgac atcctgacca ccgccgtgca ctacggcaac
     3121 cgggtgttca ccaagtaccc cgaggacatc cccgactact tcaagcagag cttccccgag
     3181 ggctacagct gggagcgcac catgaccttc gaggacaagg gcatctgcac catccgcagc
     3241 gacatcagcc tggagggcga ctgcttcttc cagaacgtgc gcttcaaggg caccaacttc
     3301 ccccccaacg gccccgtgat gcagaagaag accctgaagt gggagcccag caccgagaag
     3361 ctgcacgtgc gcgacggcct gctggtgggc aacatcaaca tggccctgct gctggagggc
     3421 ggcggccact acctgtgcga cttcaagacc acctacaagg ccaagaaggt ggtgcagctg
     3481 cccgacgccc acttcgtgga ccaccgcatc gagatcctgg gcaacgacag cgactacaac
     3541 aaggtgaagc tgtacgagca cgccgtggcc cgctacagcc ccctgcccag ccaggtgtgg
     3601 tccggactca gatcacgagc tcaagcttca aattctgcag tcgacggtac cgcgggcccg
     3661 ggatcatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     3721 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     3781 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     3841 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     3901 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     3961 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     4021 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     4081 ttcacattct tgcccgcctg atgaatgctc atccggagtt ccgtatggca atgaaagacg
     4141 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     4201 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     4261 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     4321 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     4381 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     4441 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     4501 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     4561 aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     4621 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     4681 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     4741 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     4801 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     4861 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     4921 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     4981 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     5041 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     5101 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     5161 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     5221 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     5281 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     5341 acgtttctcg ttcagctttc ttgtacaaag tggttgatga tcctaggtga gtctagagag
     5401 ttaattaaga cccgggacta gtccctagag tcctgcttta atgagatatg cgagacgcct
     5461 atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt
     5521 gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta
     5581 tcgtattttt atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat
     5641 gtacaatatt aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga
     5701 tagagcgcca caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag
     5761 cggcagaacc ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt
     5821 gccccagggg ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg
     5881 aactccggtt ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc
     5941 cgctctaccg aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg
     6001 acttgctgcc ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg
     6061 caggtcaaac cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac
     6121 aacatgtcga ggctcagcag gacctgcatg caggcatgca agcttggcac tggccgtcgt
     6181 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6241 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6301 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
     6361 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     6421 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     6481 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     6541 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
     6601 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     6661 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     6721 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     6781 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     6841 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     6901 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     6961 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     7021 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     7081 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     7141 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     7201 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     7261 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     7321 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     7381 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     7441 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     7501 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     7561 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     7621 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     7681 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     7741 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     7801 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     7861 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     7921 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     7981 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     8041 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     8101 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     8161 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     8221 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     8281 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     8341 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     8401 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     8461 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     8521 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     8581 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     8641 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     8701 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
     8761 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     8821 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     8881 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     8941 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     9001 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     9061 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     9121 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     9181 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     9241 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     9301 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     9361 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     9421 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     9481 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     9541 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     9601 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     9661 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     9721 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     9781 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     9841 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     9901 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     9961 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
    10021 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
    10081 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
    10141 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
    10201 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
    10261 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
    10321 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10381 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10441 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
    10501 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10561 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
    10621 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10681 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10741 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10801 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10861 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10921 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10981 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    11041 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    11101 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    11161 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    11221 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    11281 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    11341 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt cctgatgaat
    11401 cccctaatga ttttggtaaa aatcattaag ttaaggtgga tacacatctt gtcatatgat
    11461 caaatggttt cgcgaaaaat caataatcag acaacaagat gtgcgaactc gatattttac
    11521 acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac agcttaacgt
    11581 tggcttgcca cgcattactt gactgtaaaa ctctcactct taccgaactt ggccgtaacc
    11641 tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg ttaggtaatc
    11701 gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt atctgttcgg
    11761 gcaatacgat gcccattgta cttgttgact ggtctgatat tcgtgagcaa aaacgactta
    11821 tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat gagaaagcgt
    11881 tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc gaccttgcga
    11941 gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt aaagtgccat
    12001 ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga ggaaaagtac
    12061 aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat gatatgtcat
    12121 ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca atctcatgcc
    12181 aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg acacggactc
    12241 attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca tgggttctag
    12301 caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc tattcgaagc
    12361 gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga ctaggcctac
    12421 gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc gccctgatgc
    12481 ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg gacaagcact
    12541 tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta ggcatggaag
    12601 ttttgcggca ttctggctac acaataacaa gggaagactt actcgtggct gcaaccctac
    12661 tagctcaaaa tttattcaca catggttacg ctttggggaa attatgaggg gatctctcag
    12721 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcaatg
    12781 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
    12841 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
    12901 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
    12961 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
    13021 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
    13081 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
    13141 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
    13201 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
    13261 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
    13321 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
    13381 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
    13441 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
    13501 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
    13561 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
    13621 gtcggcaaat aataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
    13681 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
    13741 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
    13801 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
    13861 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
    13921 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
    13981 gggagctgtt ggctggctgg
//
