LOCUS       sequence-420461-       14018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            mNeonGreen fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 14018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pJRA107
FEATURES             Location/Qualifiers
     source          1..14018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    3688..3812
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3837..3842,3843..3860,3861..3867)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3921..4580
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3987..4006)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4922..5227
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5106..5125
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5268..5392)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5451..6158
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6187..6204)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6187..6203)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6196..6218)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6406..6430
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7731..8360
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8789..9862
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9928..10122
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10358..10380)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10466..10606
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10521..10540
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10621..10638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10792..11380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10872..10891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12859..13650
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gaagcaccca ccctaagcca cattttaatc cttctgttgg ccatgcccca
     1381 tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg tctgtcccga
     1441 cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc
     1501 tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac
     1561 cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt
     1621 cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga
     1681 tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg
     1741 tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg
     1801 agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag
     1861 aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata
     1921 caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa
     1981 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     2041 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     2101 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     2161 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     2221 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     2281 gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg
     2341 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     2401 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     2461 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     2521 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     2581 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     2641 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     2701 tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag
     2761 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     2821 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     2881 ctagtttgtg cgatcgaatt tgtcctcgag atggttagta agggggaaga ggataacatg
     2941 gcatcactcc cggctaccca tgaactgcac atatttggaa gtattaacgg cgttgatttt
     3001 gacatggtcg gccagggcac gggaaacccc aacgacggat atgaggagct taatcttaaa
     3061 agtacgaagg gagatttgca gttctctccc tggatactgg tcccgcatat cgggtacggt
     3121 ttccatcaat atctgcccta ccccgacggt atgagtcctt tccaggctgc aatggtggac
     3181 ggctctggct accaagttca taggacgatg caattcgagg acggtgcaag tcttacagtc
     3241 aattatagat acacatatga gggttctcac ataaagggag aggcccaggt gaaagggacg
     3301 ggcttccccg ctgatggccc cgtgatgaca aactcactca cggcagccga ctggtgtagg
     3361 tctaaaaaga cttaccctaa cgacaagacg attatatcaa cattcaaatg gagctacacc
     3421 actgggaatg gcaagcgtta cagaagtact gccaggacaa catacacttt tgcgaagccc
     3481 atggctgcta actatctaaa aaaccaacca atgtatgttt tcagaaaaac cgagcttaag
     3541 cacagtaaga ctgaactaaa ctttaaagag tggcaaaagg cttttacgga tgtcatgggt
     3601 atggacgagc tttacaagtc cggactcaga tcacgagctc aagcttcaaa ttctgcagtc
     3661 gacggtaccg cgggcccggg atcatcaaca agtttgtaca aaaaagctga acgagaaacg
     3721 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     3781 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
     3841 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
     3901 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     3961 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     4021 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     4081 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggagttcc
     4141 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     4201 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     4261 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     4321 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     4381 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     4441 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     4501 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     4561 agtggcaggg cggggcgtaa agatctggat ccggcttact aaaagccaga taacagtatg
     4621 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     4681 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     4741 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     4801 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     4861 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
     4921 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     4981 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
     5041 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     5101 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     5161 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     5221 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
     5281 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     5341 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gttgatgatc
     5401 ctaggtgagt ctagagagtt aattaagacc cgggactagt ccctagagtc ctgctttaat
     5461 gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg
     5521 taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga
     5581 atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt
     5641 gtaccctact acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt
     5701 ttatagcgac atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa
     5761 atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct
     5821 tattcaaatt tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta
     5881 ataacgctca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga
     5941 agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc
     6001 acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg
     6061 tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc
     6121 cagggcgaat tttgcgacaa catgtcgagg ctcagcagga cctgcatgca ggcatgcaag
     6181 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     6241 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     6301 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt
     6361 ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg
     6421 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     6481 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     6541 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     6601 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     6661 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     6721 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     6781 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     6841 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     6901 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     6961 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     7021 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     7081 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     7141 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     7201 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     7261 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     7321 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     7381 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     7441 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     7501 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     7561 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     7621 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     7681 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     7741 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     7801 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     7861 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     7921 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     7981 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     8041 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     8101 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     8161 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     8221 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     8281 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     8341 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     8401 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     8461 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     8521 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     8581 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     8641 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     8701 gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca
     8761 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
     8821 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     8881 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     8941 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     9001 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     9061 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     9121 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     9181 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     9241 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     9301 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     9361 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     9421 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     9481 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     9541 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     9601 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     9661 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     9721 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     9781 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     9841 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     9901 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     9961 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
    10021 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
    10081 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
    10141 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
    10201 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
    10261 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
    10321 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10381 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10441 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
    10501 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10561 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
    10621 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10681 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10741 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10801 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10861 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10921 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10981 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11041 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11101 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11161 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11221 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11281 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11341 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11401 tacggggtcc tgatgaatcc cctaatgatt ttggtaaaaa tcattaagtt aaggtggata
    11461 cacatcttgt catatgatca aatggtttcg cgaaaaatca ataatcagac aacaagatgt
    11521 gcgaactcga tattttacac gactctcttt accaattctg ccccgaatta cacttaaaac
    11581 gactcaacag cttaacgttg gcttgccacg cattacttga ctgtaaaact ctcactctta
    11641 ccgaacttgg ccgtaacctg ccaaccaaag cgagaacaaa acataacatc aaacgaatcg
    11701 accgattgtt aggtaatcgt cacctccaca aagagcgact cgctgtatac cgttggcatg
    11761 ctagctttat ctgttcgggc aatacgatgc ccattgtact tgttgactgg tctgatattc
    11821 gtgagcaaaa acgacttatg gtattgcgag cttcagtcgc actacacggt cgttctgtta
    11881 ctctttatga gaaagcgttc ccgctttcag agcaatgttc aaagaaagct catgaccaat
    11941 ttctagccga ccttgcgagc attctaccga gtaacaccac accgctcatt gtcagtgatg
    12001 ctggctttaa agtgccatgg tataaatccg ttgagaagct gggttggtac tggttaagtc
    12061 gagtaagagg aaaagtacaa tatgcagacc taggagcgga aaactggaaa cctatcagca
    12121 acttacatga tatgtcatct agtcactcaa agactttagg ctataagagg ctgactaaaa
    12181 gcaatccaat ctcatgccaa attctattgt ataaatctcg ctctaaaggc cgaaaaaatc
    12241 agcgctcgac acggactcat tgtcaccacc cgtcacctaa aatctactca gcgtcggcaa
    12301 aggagccatg ggttctagca actaacttac ctgttgaaat tcgaacaccc aaacaacttg
    12361 ttaatatcta ttcgaagcga atgcagattg aagaaacctt ccgagacttg aaaagtcctg
    12421 cctacggact aggcctacgc catagccgaa cgagcagctc agagcgtttt gatatcatgc
    12481 tgctaatcgc cctgatgctt caactaacat gttggcttgc gggcgttcat gctcagaaac
    12541 aaggttggga caagcacttc caggctaaca cagtcagaaa tcgaaacgta ctctcaacag
    12601 ttcgcttagg catggaagtt ttgcggcatt ctggctacac aataacaagg gaagacttac
    12661 tcgtggctgc aaccctacta gctcaaaatt tattcacaca tggttacgct ttggggaaat
    12721 tatgagggga tctctcagta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    12781 gattttggtc atgcaatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
    12841 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
    12901 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
    12961 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
    13021 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
    13081 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
    13141 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
    13201 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
    13261 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
    13321 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
    13381 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
    13441 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
    13501 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
    13561 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
    13621 ggcgagatca ccaaggtagt cggcaaataa taacaagacg aactccaatt cactgttcct
    13681 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
    13741 gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct
    13801 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
    13861 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
    13921 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
    13981 gtgccgagct gccggtcggg gagctgttgg ctggctgg
//
