LOCUS       sequence-420462-       14019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            mCherry fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 14019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pJRA108
FEATURES             Location/Qualifiers
     source          1..14019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2912..3619
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3325..3344
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    3689..3813
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3838..3843,3844..3861,3862..3868)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3922..4581
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3988..4007)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4923..5228
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5107..5126
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5269..5393)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5452..6159
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6188..6205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6188..6204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6197..6219)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6407..6431
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7732..8361
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8790..9863
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9929..10123
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10359..10381)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10467..10607
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10522..10541
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10622..10639)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10793..11381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10873..10892)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12860..13651
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gaagcaccca ccctaagcca cattttaatc cttctgttgg ccatgcccca
     1381 tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg tctgtcccga
     1441 cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc
     1501 tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac
     1561 cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt
     1621 cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga
     1681 tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg
     1741 tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg
     1801 agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag
     1861 aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata
     1921 caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa
     1981 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     2041 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     2101 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     2161 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     2221 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     2281 gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg
     2341 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     2401 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     2461 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     2521 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     2581 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     2641 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     2701 tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag
     2761 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     2821 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     2881 ctagtttgtg cgatcgaatt tgtcctcgag aatggtgagc aagggcgagg aggataacat
     2941 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3001 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3061 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3121 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     3181 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     3241 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     3301 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3361 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     3421 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     3481 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     3541 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     3601 catggacgag ctgtacaagt ccggactcag atcacgagct caagcttcaa attctgcagt
     3661 cgacggtacc gcgggcccgg gatcatcaac aagtttgtac aaaaaagctg aacgagaaac
     3721 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     3781 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt
     3841 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
     3901 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     3961 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     4021 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     4081 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggagttc
     4141 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     4201 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     4261 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     4321 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     4381 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     4441 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     4501 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     4561 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     4621 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     4681 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct
     4741 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     4801 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     4861 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg
     4921 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     4981 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     5041 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     5101 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     5161 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     5221 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     5281 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     5341 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgatgat
     5401 cctaggtgag tctagagagt taattaagac ccgggactag tccctagagt cctgctttaa
     5461 tgagatatgc gagacgccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt
     5521 gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg
     5581 aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca atttactgat
     5641 tgtaccctac tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg
     5701 tttatagcga catctatgat agagcgccac aataacaaac aattgcgttt tattattaca
     5761 aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc
     5821 ttattcaaat ttcaaaagtg ccccaggggc tagtatctac gacacaccga gcggcgaact
     5881 aataacgctc actgaaggga actccggttc cccgccggcg cgcatgggtg agattccttg
     5941 aagttgagta ttggccgtcc gctctaccga aagttacggg caccattcaa cccggtccag
     6001 cacggcggcc gggtaaccga cttgctgccc cgagaattat gcagcatttt tttggtgtat
     6061 gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt
     6121 ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg acctgcatgc aggcatgcaa
     6181 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     6241 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     6301 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc agcttgagct
     6361 tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt
     6421 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
     6481 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     6541 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     6601 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     6661 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     6721 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     6781 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     6841 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     6901 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     6961 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     7021 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     7081 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     7141 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     7201 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     7261 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     7321 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     7381 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     7441 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     7501 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     7561 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     7621 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     7681 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     7741 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     7801 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     7861 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     7921 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     7981 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     8041 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     8101 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     8161 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     8221 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     8281 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     8341 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     8401 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     8461 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     8521 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     8581 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     8641 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     8701 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc
     8761 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
     8821 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     8881 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     8941 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     9001 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     9061 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     9121 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     9181 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     9241 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     9301 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     9361 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     9421 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     9481 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     9541 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     9601 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     9661 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     9721 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     9781 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     9841 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     9901 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     9961 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
    10021 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
    10081 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
    10141 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
    10201 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
    10261 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
    10321 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
    10381 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    10441 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
    10501 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10561 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
    10621 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10681 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10741 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10801 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10861 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10921 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10981 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    11041 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    11101 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    11161 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    11221 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    11281 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    11341 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    11401 ctacggggtc ctgatgaatc ccctaatgat tttggtaaaa atcattaagt taaggtggat
    11461 acacatcttg tcatatgatc aaatggtttc gcgaaaaatc aataatcaga caacaagatg
    11521 tgcgaactcg atattttaca cgactctctt taccaattct gccccgaatt acacttaaaa
    11581 cgactcaaca gcttaacgtt ggcttgccac gcattacttg actgtaaaac tctcactctt
    11641 accgaacttg gccgtaacct gccaaccaaa gcgagaacaa aacataacat caaacgaatc
    11701 gaccgattgt taggtaatcg tcacctccac aaagagcgac tcgctgtata ccgttggcat
    11761 gctagcttta tctgttcggg caatacgatg cccattgtac ttgttgactg gtctgatatt
    11821 cgtgagcaaa aacgacttat ggtattgcga gcttcagtcg cactacacgg tcgttctgtt
    11881 actctttatg agaaagcgtt cccgctttca gagcaatgtt caaagaaagc tcatgaccaa
    11941 tttctagccg accttgcgag cattctaccg agtaacacca caccgctcat tgtcagtgat
    12001 gctggcttta aagtgccatg gtataaatcc gttgagaagc tgggttggta ctggttaagt
    12061 cgagtaagag gaaaagtaca atatgcagac ctaggagcgg aaaactggaa acctatcagc
    12121 aacttacatg atatgtcatc tagtcactca aagactttag gctataagag gctgactaaa
    12181 agcaatccaa tctcatgcca aattctattg tataaatctc gctctaaagg ccgaaaaaat
    12241 cagcgctcga cacggactca ttgtcaccac ccgtcaccta aaatctactc agcgtcggca
    12301 aaggagccat gggttctagc aactaactta cctgttgaaa ttcgaacacc caaacaactt
    12361 gttaatatct attcgaagcg aatgcagatt gaagaaacct tccgagactt gaaaagtcct
    12421 gcctacggac taggcctacg ccatagccga acgagcagct cagagcgttt tgatatcatg
    12481 ctgctaatcg ccctgatgct tcaactaaca tgttggcttg cgggcgttca tgctcagaaa
    12541 caaggttggg acaagcactt ccaggctaac acagtcagaa atcgaaacgt actctcaaca
    12601 gttcgcttag gcatggaagt tttgcggcat tctggctaca caataacaag ggaagactta
    12661 ctcgtggctg caaccctact agctcaaaat ttattcacac atggttacgc tttggggaaa
    12721 ttatgagggg atctctcagt acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    12781 ggattttggt catgcaatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
    12841 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
    12901 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
    12961 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
    13021 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
    13081 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
    13141 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
    13201 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
    13261 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
    13321 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
    13381 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
    13441 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
    13501 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
    13561 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
    13621 aggcgagatc accaaggtag tcggcaaata ataacaagac gaactccaat tcactgttcc
    13681 ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg
    13741 cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc
    13801 tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg
    13861 cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac
    13921 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
    13981 tgtgccgagc tgccggtcgg ggagctgttg gctggctgg
//
