LOCUS       sequence-420463-       14021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            mTagBFP2 fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14021)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 14021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pJRA109
FEATURES             Location/Qualifiers
     source          1..14021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    3691..3815
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3840..3845,3846..3863,3864..3870)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3924..4583
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3990..4009)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4925..5230
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5109..5128
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5271..5395)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5454..6161
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6190..6207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6190..6206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6199..6221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6409..6433
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7734..8363
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8792..9865
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9931..10125
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10361..10383)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10469..10609
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10524..10543
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10624..10641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10795..11383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10875..10894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12862..13653
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gaagcaccca ccctaagcca cattttaatc cttctgttgg ccatgcccca
     1381 tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg tctgtcccga
     1441 cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc
     1501 tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac
     1561 cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt
     1621 cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga
     1681 tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg
     1741 tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg
     1801 agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag
     1861 aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata
     1921 caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa
     1981 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     2041 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     2101 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     2161 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     2221 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     2281 gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg
     2341 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     2401 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     2461 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     2521 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     2581 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     2641 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     2701 tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag
     2761 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     2821 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     2881 ctagtttgtg cgatcgaatt tgtcctcgag atggtttcta agggagaaga acttattaaa
     2941 gaaaacatgc atatgaaact gtacatggag ggaactgttg ataaccatca tttcaagtgt
     3001 acttcagagg gtgagggtaa gccttacgag ggtactcaaa ccatgagaat taaggtggtt
     3061 gaaggaggac ctcttccttt tgcttttgat attcttgcta cctctttctt gtacggttca
     3121 aagacattta ttaaccatac gcagggaatc cctgacttct ttaaacagtc tttccctgaa
     3181 gggttcacct gggaaagggt tactacatat gaagatggtg gagtgttgac cgcaacccag
     3241 gatacctctc ttcaagatgg atgtcttatc tacaatgtta agatcagagg agttaacttt
     3301 actagtaacg gaccagttat gcagaagaag acacttggat gggaggcttt tacagagaca
     3361 ttgtatcctg ctgatggtgg attggaaggt agaaatgata tggctttgaa actcgttggt
     3421 ggttcacatt tgattgctaa tgctaagaca acttacagat ctaaaaaacc agctaagaac
     3481 ttgaagatgc ctggagtgta ctacgttgat tatagattag aaagaattaa ggaggctaat
     3541 aatgagacat acgttgaaca gcatgaggtg gctgttgcta gatattgtga tcttcctagc
     3601 aagttgggac ataagttaaa ctccggactc agatcacgag ctcaagcttc aaattctgca
     3661 gtcgacggta ccgcgggccc gggatcatca acaagtttgt acaaaaaagc tgaacgagaa
     3721 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     3781 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     3841 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     3901 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     3961 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     4021 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     4081 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggagt
     4141 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     4201 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     4261 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     4321 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     4381 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     4441 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     4501 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     4561 atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt
     4621 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     4681 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     4741 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     4801 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     4861 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     4921 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     4981 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     5041 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     5101 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     5161 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     5221 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     5281 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     5341 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgatg
     5401 atcctaggtg agtctagaga gttaattaag acccgggact agtccctaga gtcctgcttt
     5461 aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg
     5521 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa
     5581 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg
     5641 attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata
     5701 ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta
     5761 caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa
     5821 tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa
     5881 ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct
     5941 tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc
     6001 agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt
     6061 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
     6121 gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcat gcaggcatgc
     6181 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6241 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6301 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     6361 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     6421 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     6481 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     6541 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     6601 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     6661 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     6721 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     6781 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     6841 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     6901 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     6961 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     7021 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     7081 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     7141 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     7201 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     7261 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     7321 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     7381 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     7441 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     7501 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     7561 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     7621 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     7681 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     7741 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     7801 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     7861 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     7921 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     7981 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     8041 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     8101 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     8161 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     8221 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     8281 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     8341 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     8401 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     8461 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     8521 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     8581 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     8641 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     8701 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
     8761 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     8821 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     8881 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     8941 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     9001 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     9061 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     9121 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     9181 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     9241 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     9301 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     9361 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     9421 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     9481 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     9541 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     9601 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     9661 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     9721 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     9781 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     9841 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     9901 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     9961 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
    10021 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
    10081 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
    10141 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
    10201 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
    10261 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
    10321 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    10381 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    10441 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
    10501 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10561 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
    10621 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10681 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10741 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10801 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10861 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10921 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10981 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11041 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11101 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11161 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11221 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11281 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11341 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11401 ttctacgggg tcctgatgaa tcccctaatg attttggtaa aaatcattaa gttaaggtgg
    11461 atacacatct tgtcatatga tcaaatggtt tcgcgaaaaa tcaataatca gacaacaaga
    11521 tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa
    11581 aacgactcaa cagcttaacg ttggcttgcc acgcattact tgactgtaaa actctcactc
    11641 ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa
    11701 tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc
    11761 atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata
    11821 ttcgtgagca aaaacgactt atggtattgc gagcttcagt cgcactacac ggtcgttctg
    11881 ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc
    11941 aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg
    12001 atgctggctt taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa
    12061 gtcgagtaag aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca
    12121 gcaacttaca tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta
    12181 aaagcaatcc aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa
    12241 atcagcgctc gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg
    12301 caaaggagcc atgggttcta gcaactaact tacctgttga aattcgaaca cccaaacaac
    12361 ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc
    12421 ctgcctacgg actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca
    12481 tgctgctaat cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga
    12541 aacaaggttg ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa
    12601 cagttcgctt aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact
    12661 tactcgtggc tgcaacccta ctagctcaaa atttattcac acatggttac gctttgggga
    12721 aattatgagg ggatctctca gtacggggtc tgacgctcag tggaacgaaa actcacgtta
    12781 agggattttg gtcatgcaat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
    12841 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
    12901 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
    12961 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
    13021 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
    13081 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
    13141 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
    13201 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
    13261 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
    13321 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
    13381 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
    13441 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
    13501 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
    13561 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
    13621 aaaggcgaga tcaccaaggt agtcggcaaa taataacaag acgaactcca attcactgtt
    13681 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    13741 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
    13801 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
    13861 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
    13921 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
    13981 tttgtgccga gctgccggtc ggggagctgt tggctggctg g
//
