LOCUS       sequence-420465-       13868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            SNAP-tag fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 13868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pUBQ10-SNAP-GW
FEATURES             Location/Qualifiers
     source          1..13868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2922..3467
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     protein_bind    3537..3661
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3686..3691,3692..3709,3710..3716)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3770..4429
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3836..3855)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4771..5076
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4955..4974
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5117..5241)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5300..6007
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6032..6049)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6032..6048)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6041..6063)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6251..6275
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7576..8205
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8634..9707
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9773..9967
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10203..10225)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10311..10451
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10366..10385
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10466..10483)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10637..11225)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10717..10736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12650..13444)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gtatgatcgc gaagcaccca ccctaagcca cattttaatc cttctgttgg
     1381 ccatgcccca tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg
     1441 tctgtcccga cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa
     1501 tccactcatc tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc
     1561 ctttaagcac cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag
     1621 cctccacatt cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg
     1681 tatgaactga tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga
     1741 atgttttgtg tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg
     1801 actgaacacg agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt
     1861 aaataacaag aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac
     1921 caacttgata caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata
     1981 acactaaaaa attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac
     2041 ttttccaaga aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa
     2101 acaaaaagga aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg
     2161 cagtgggacc cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata
     2221 aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct
     2281 tatgacgacc gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg
     2341 ttgcagccgg cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac
     2401 ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg
     2461 tcattttatt attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg
     2521 tcttttcttc ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca
     2581 gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt
     2641 aaatttctgt gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt
     2701 tcgtatatgt tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt
     2761 tgatcgttag atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt
     2821 caaataattt gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg
     2881 gtgttagttt ctagtttgtg cgatcgaatt tgtcctcgag aatggacaaa gactgcgaaa
     2941 tgaagcgcac caccctggat agccctctgg gcaagctgga actgtctggg tgcgaacagg
     3001 gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc gtggaagtgc
     3061 ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc gcctggctca
     3121 acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc ctgcaccacc
     3181 cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg aaagtggtga
     3241 agttcggaga ggtcatcagc tacagccacc tggccgccct ggccggcaat cccgccgcca
     3301 ccgccgccgt gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc ccctgccacc
     3361 gggtggtgca gggcgacctg gacgtggggg gctacgaggg cgggctcgcc gtgaaagagt
     3421 ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggttcc ggactcagat
     3481 cacgagctca agcttcaaat tctgcagtcg acggtaccgc gggcccggga tcatcaacaa
     3541 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     3601 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     3661 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     3721 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     3781 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     3841 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     3901 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     3961 ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg agctggtgat
     4021 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     4081 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     4141 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     4201 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     4261 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     4321 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     4381 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc
     4441 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
     4501 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt
     4561 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
     4621 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
     4681 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
     4741 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga
     4801 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
     4861 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     4921 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     4981 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     5041 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
     5101 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     5161 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     5221 agctttcttg tacaaagtgg ttgatgatcc taggtgagtc tagagagtta attaagaccc
     5281 gggactagtc cctagagtcc tgctttaatg agatatgcga gacgcctatg atcgcatgat
     5341 atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc
     5401 ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg tatttttatg
     5461 aataatattc tccgttcaat ttactgattg taccctacta cttatatgta caatattaaa
     5521 atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag agcgccacaa
     5581 taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg cagaaccggt
     5641 caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc ccaggggcta
     5701 gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac tccggttccc
     5761 cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc tctaccgaaa
     5821 gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact tgctgccccg
     5881 agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt
     5941 gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc
     6001 tcagcaggac ctgcaggcat gcacgcttgg cactggccgt cgttttacaa cgtcgtgact
     6061 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6121 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6181 gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
     6241 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     6301 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     6361 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
     6421 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
     6481 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
     6541 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
     6601 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
     6661 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     6721 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     6781 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     6841 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     6901 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     6961 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     7021 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     7081 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     7141 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     7201 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     7261 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     7321 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
     7381 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     7441 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     7501 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     7561 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     7621 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     7681 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     7741 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     7801 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     7861 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     7921 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     7981 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     8041 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     8101 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     8161 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     8221 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     8281 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     8341 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     8401 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     8461 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     8521 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     8581 gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     8641 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     8701 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     8761 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     8821 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     8881 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     8941 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     9001 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     9061 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     9121 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     9181 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     9241 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     9301 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     9361 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     9421 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     9481 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     9541 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     9601 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     9661 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     9721 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     9781 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     9841 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     9901 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     9961 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
    10021 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
    10081 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
    10141 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
    10201 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    10261 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
    10321 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
    10381 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    10441 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10501 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10561 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10621 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10681 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10741 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10801 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10861 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10921 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10981 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11041 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    11101 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11161 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11221 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11281 gttaagggct gagagatccc ctcataattt ccccaaagcg taaccatgtg tgaataaatt
    11341 ttgagctagt agggttgcag ccacgagtaa gtcttccctt gttattgtgt agccagaatg
    11401 ccgcaaaact tccatgccta agcgaactgt tgagagtacg tttcgatttc tgactgtgtt
    11461 agcctggaag tgcttgtccc aaccttgttt ctgagcatga acgcccgcaa gccaacatgt
    11521 tagttgaagc atcagggcga ttagcagcat gatatcaaaa cgctctgagc tgctcgttcg
    11581 gctatggcgt aggcctagtc cgtaggcagg acttttcaag tctcggaagg tttcttcaat
    11641 ctgcattcgc ttcgaataga tattaacaag ttgtttgggt gttcgaattt caacaggtaa
    11701 gttagttgct agaatccatg gctcctttgc cgacgctgag tagattttag gtgacgggtg
    11761 gtgacaatga gtccgtgtcg agcgctgatt ttttcggcct ttagagcgag atttatacaa
    11821 tagaatttgg catgagattg gattgctttt agtcagcctc ttatagccta aagtctttga
    11881 gtgactagat gacatatcat gtaagttgct gataggtttc cagttttccg ctcctaggtc
    11941 tgcatattgt acttttcctc ttactcgact taaccagtac caacccagct tctcaacgga
    12001 tttataccat ggcactttaa agccagcatc actgacaatg agcggtgtgg tgttactcgg
    12061 tagaatgctc gcaaggtcgg ctagaaattg gtcatgagct ttctttgaac attgctctga
    12121 aagcgggaac gctttctcat aaagagtaac agaacgaccg tgtagtgcga ctgaagctcg
    12181 caataccata agccgttttt gctcacggat atcagaccag tcaacaagta caatgggcat
    12241 cgtattgccc gaacagataa agctagcatg ccaacggtat acagcgagtc gctctttgtg
    12301 gaggtgacga ttacctaaca atcggtcgat tcgtttgatg ttatgttttg ttctcgcttt
    12361 ggttggcagg ttacggccaa gttcggtaag agtgagagtt ttacagtcaa gtaaggcgtg
    12421 gcaagccaac gttaagctgt tgagtcgttt taagtgtaat tcggggcaga attggtaaag
    12481 agagtcgtgt aaaatatcga gttcgcacat tttgttgtct gattattgat ttttggcgaa
    12541 accatttgat catatgacaa gatgtgtatc taccttaact taatgatttt gataaaaatc
    12601 attaggggat tcatcagcgt taagggattt tggtcatgca ttctaggtac taaaacaatt
    12661 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
    12721 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
    12781 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    12841 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    12901 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    12961 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    13021 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    13081 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    13141 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    13201 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    13261 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    13321 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    13381 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    13441 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    13501 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    13561 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    13621 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    13681 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    13741 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
    13801 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
    13861 ctggctgg
//
