LOCUS       sequence-420477-       10697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human TopBP1 C-terminally fused to 
            mCerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10697)
  TITLE     Pospiech Lab Plasmids 2023
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-TopBP1-mCer
FEATURES             Location/Qualifiers
     source          1..10697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        231..610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     765..785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     859..878
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        859..877
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(4531..4542)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      5511..5520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5517..5519,5520..5522,5523..6236)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5562..5583)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5823..5842)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6170..6191
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(6246..6264)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6247..6264)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6284..6301)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6290..6514
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6560..6988
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6647..6666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6857..6878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6997..7017)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7002..7331
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7182..7317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7244..7263
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7398..8192
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7452..7471)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8062..8081
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8366..8487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8403..8422)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8457..8476
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8536..8552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8536..8552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8549..8571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8560..8576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8584..8590,8591..8608,8609..8614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8629..8650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8767..8784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8938..9526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9018..9037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9697..10488,10489..10557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10320..10339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10558..10662)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
       61 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
      121 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      181 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      241 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      301 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      361 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      421 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      481 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      541 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      601 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      661 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      721 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      781 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      841 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt ggtaccgagc
      901 tcggatccat gtccagaaat gacaaagaac cgttttttgt gaagttttta aagtcttcag
      961 acaattccaa atgttttttt aaagctctcg agtccataaa agaattccaa tcagaagaat
     1021 atcttcagat tattacagaa gaagaggcat tgaagataaa ggagaatgat agatcacttt
     1081 atatctgtga cccttttagt ggcgttgtct ttgatcacct caaaaagctt ggctgcagaa
     1141 ttgttggtcc tcaagtagtc atattttgta tgcaccacca gcgatgtgtc ccaagagccg
     1201 aacatccagt ttataatatg gttatgtctg atgtaaccat atcttgtaca agtctggaaa
     1261 aagaaaaaag ggaagaagtt cataaatatg tacaaatgat gggcggacga gtatacagag
     1321 accttaatgt atcagtaact caccttattg caggagaagt tggtagcaaa aaatatttag
     1381 ttgctgcaaa cctgaagaaa cctattttgc ttccctcttg gataaaaaca ctttgggaga
     1441 agtcacaaga gaaaaaaata actagatata ctgatataaa catggaagat ttcaagtgtc
     1501 ctatttttct tggttgcata atctgtgtga ctggcttatg tggcttagac aggaaagaag
     1561 ttcagcaact cacagttaag catggaggtc aatacatggg acaattgaaa atgaatgaat
     1621 gtacacacct cattgtgcaa gaaccaaaag gtcagaagta tgagtgtgcc aagagatgga
     1681 atgtacactg tgtgaccaca cagtggtttt ttgacagtat tgagaaaggt ttttgtcagg
     1741 atgaatccat atacaagaca gaacctagac cagaagcaaa gactatgccc aattcttcaa
     1801 ctcctaccag ccagatcaac acaattgata gtcgtactct ttcagatgtc agcaatattt
     1861 ccaacataaa tgcaagttgc gtaagtgaat caatatgtaa ttcacttaac agcaaactgg
     1921 agcctacact tgaaaatcta gaaaatctgg atgtcagtgc atttcaagca cctgaagatt
     1981 tattagatgg ttgtcggata tatctttgcg gttttagtgg cagaaagcta gataaactga
     2041 gaagacttat taacagtgga ggtggagttc gttttaacca gctaaatgaa gatgtaactc
     2101 atgttattgt gggagattat gatgatgaat tgaagcagtt ttggaataaa tcagcccaca
     2161 ggcctcatgt agtgggagca aagtggttgc tagagtgttt cagtaaaggt tatatgcttt
     2221 ctgaagaacc atatatccat gctaattacc agccagtgga aattccagtt tcacatcagc
     2281 ctgaaagtaa agcagctctt ttaaaaaaga agaacagcag cttctctaag aaagactttg
     2341 ctcctagtga aaagcatgag caagctgatg aagatctgct ctctcaatat gaaaatggta
     2401 gctccacagt agttgaggct aagacgtctg aagccaggcc ctttaatgat tctactcatg
     2461 ctgagccctt gaatgattct actcacattt ctttgcaaga agaaaaccag tcttctgtca
     2521 gtcattgtgt ccctgatgtt tctacaatta ctgaagaagg cttatttagc caaaagagtt
     2581 tccttgtttt gggttttagt aatgaaaatg aatctaacat cgcaaacatc ataaaagaaa
     2641 atgctgggaa aatcatgtcc cttctgagca gaactgttgc ggattatgct gtggttcctc
     2701 tgctggggtg tgaagtggaa gccactgtgg gagaagttgt tacaaataca tggctggtta
     2761 cttgcataga ctatcagact ttgtttgatc caaagtcgaa tcctctcttc acaccagttc
     2821 cagtaatgac aggaatgact cctttagagg attgtgttat ttcatttagc cagtgtgctg
     2881 gagcagaaaa agagtcttta actttcctag caaacctcct tggagcaagt gttcaagaat
     2941 actttgttcg caaatccaat gcaaagaaag gcatgtttgc cagtactcat cttatactga
     3001 aagaacgtgg tggctctaaa tatgaagctg caaagaagtg gaatttacct gccgttacta
     3061 tagcttggct gttggagact gctagaacgg gaaagagagc agacgaaagc cattttctga
     3121 ttgaaaattc aactaaagaa gaacgaagtt tggaaacaga aataacaaat ggaatcaatc
     3181 taaattcaga tactgcagag catcctggca cacgcctgca aactcacaga aaaaccgtcg
     3241 ttacaccttt agatatgaac cgctttcaga gtaaagcttt ccgtgctgtg gtctcacaac
     3301 atgccagaca ggtcgcagcc tccccagcag taggacaacc acttcagaag gagccctcgt
     3361 tacacctgga tacaccatca aaattcctgt ccaaggacaa actcttcaag ccttcctttg
     3421 atgtgaagga tgcacttgca gccttggaaa ctccaggacg tcccagccaa cagaaaagga
     3481 aaccgagtac gccactctca gaagttattg tcaaaaactt gcaacttgct ttggcaaata
     3541 gctctcgaaa tgctgtcgct ctttctgcca gccctcaact gaaagaggcc cagtcagaga
     3601 aggaagaagc cccaaagcca cttcacaaag tagtggtatg tgttagtaaa aaactcagta
     3661 agaagcagag tgaactaaat gggatcgcag cctctctagg agcagattac aggtggagtt
     3721 ttgatgaaac agtgactcat ttcatctatc aagggcggcc aaatgacact aatcgggagt
     3781 ataaatctgt aaaagaaaga ggagtacaca ttgtttccga gcactggctt ttagattgtg
     3841 cccaagagtg taaacatctt cctgaatctc tttatccaca tacttataat cccaaaatga
     3901 gcttggatat cagcgcagtg caagatggcc ggctctgtaa tagtcgacta ctctcagctg
     3961 tgtcttcaac aaaggatgat gagccagatc ctttgatttt agaagaaaat gatgtagaca
     4021 atatggccac caataataaa gagtcagcac catcaaatgg aagtggaaag aatgactcta
     4081 aaggagttct gacacagacc ttagagatga gagagaactt tcagaagcag ttacaggaga
     4141 taatgtctgc aacatcaata gtgaaacccc aagggcagag gacttccctt tcaagaagtg
     4201 gttgtaacag cgcatcttca acccctgaca gcactcgctc tgctcgcagt ggacgaagta
     4261 gagtcctaga ggcactgagg cagtctcgtc agacagtacc tgatgtcaac acagagcctt
     4321 cccaaaatga acagatcatt tgggatgacc ctacagcaag ggaggagaga gcaaggcttg
     4381 ccagcaattt gcagtggcct agttgtccca cacaatactc tgagcttcag gttgacattc
     4441 aaaacttgga ggattctcct tttcaaaagc ctttacatga ttcagaaatt gctaaacagg
     4501 ctgtctgtga tcctggaaac atacgtgtga ctgaagctcc caaacaccca atctctgaag
     4561 aactggaaac tcccataaaa gacagccacc tgatccctac gcctcaagcc cccagtattg
     4621 cctttccact cgccaacccc cctgtggctc cgcaccctag agaaaagatt ataacgatag
     4681 aggagactca tgaagaatta aaaaaacagt acatatttca gttatcatct ctgaatcctc
     4741 aagaacgtat tgactattgt catctgattg agaaactagg tggattggtg atagaaaagc
     4801 agtgctttga tcccacctgt acacacattg ttgtgggaca tccacttcga aacgagaagt
     4861 atttagcctc agtggcagct gggaagtggg tgcttcatcg ctcctacctt gaagcctgca
     4921 ggactgctgg acacttcgtg caggaagaag actatgaatg gggaagtagt tccatacttg
     4981 atgttctgac tggaatcaat gtacagcaac gaagactagc acttgcagca atgagatgga
     5041 gaaaaaaaat ccagcaaaga caagaatctg gcattgttga gggagcattt agtgggtgga
     5101 aggttatttt acatgtggat cagtctcgag aagcaggctt caaacgcctt cttcagtcag
     5161 gaggagcaaa ggtgctacct ggtcattctg tacctttatt taaagaggcc acacatcttt
     5221 tttctgactt gaataaactg aaaccagatg actcaggagt taatatagca gaagctgctg
     5281 cccagaacgt gtactgcttg agaacagaat acattgctga ttatctcatg caggaatcac
     5341 ctcctcatgt agaaaattac tgtctaccag aagctatttc atttattcag aataataagg
     5401 aacttgggac tggattatca caaaagagga aagctcctac agaaaaaaat aaaatcaaac
     5461 gacctagagt acactcgcgg ccgctcgagc atgcatctag aagatccatc gccaccatgg
     5521 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     5581 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     5641 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5701 tgaccaccct gacctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     5761 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5821 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5881 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5941 tggagtacaa cgccatcagc gacaacgtct atatcaccgc cgacaagcag aagaacggca
     6001 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     6061 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6121 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     6181 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagggc
     6241 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
     6301 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6361 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6421 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6481 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6541 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6601 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6661 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6721 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6781 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6841 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6901 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6961 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     7021 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7081 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7141 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7201 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7261 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7321 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     7381 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     7441 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7501 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7561 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7621 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7681 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     7741 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7801 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7861 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7921 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7981 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     8041 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     8101 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     8161 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     8221 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     8281 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8341 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8401 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8461 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8521 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8581 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8641 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8701 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8761 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9361 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9661 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9721 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9781 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9841 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9901 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9961 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10021 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10081 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10141 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10201 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10261 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10321 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10381 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10441 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10501 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10561 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10621 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10681 gccacctgac gtcgacg
//
