LOCUS       sequence-420488-       12875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            HaloTag fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12875)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 12875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pUBQ10-HALO-GW
FEATURES             Location/Qualifiers
     source          1..12875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2922..3812
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     protein_bind    3882..4006
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4031..4036,4037..4054,4055..4061)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4115..4774
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4181..4200)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5116..5421
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5300..5319
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5462..5586)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5645..6352
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6377..6394)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6377..6393)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6386..6408)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6596..6620
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7921..8550
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8979..10052
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10118..10312
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10548..10570)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10656..10796
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10711..10730
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10811..10828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10982..11570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11062..11081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11657..12451)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gtatgatcgc gaagcaccca ccctaagcca cattttaatc cttctgttgg
     1381 ccatgcccca tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg
     1441 tctgtcccga cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa
     1501 tccactcatc tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc
     1561 ctttaagcac cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag
     1621 cctccacatt cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg
     1681 tatgaactga tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga
     1741 atgttttgtg tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg
     1801 actgaacacg agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt
     1861 aaataacaag aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac
     1921 caacttgata caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata
     1981 acactaaaaa attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac
     2041 ttttccaaga aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa
     2101 acaaaaagga aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg
     2161 cagtgggacc cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata
     2221 aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct
     2281 tatgacgacc gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg
     2341 ttgcagccgg cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac
     2401 ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg
     2461 tcattttatt attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg
     2521 tcttttcttc ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca
     2581 gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt
     2641 aaatttctgt gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt
     2701 tcgtatatgt tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt
     2761 tgatcgttag atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt
     2821 caaataattt gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg
     2881 gtgttagttt ctagtttgtg cgatcgaatt tgtcctcgag aatggcagaa atcggtactg
     2941 gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg
     3001 ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc tcctcctacg
     3061 tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct ccagacctga
     3121 tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac cacgtccgct
     3181 tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc attcacgact
     3241 ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc aaaggtattg
     3301 catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa tttgcccgcg
     3361 agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc gatcagaacg
     3421 tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg
     3481 accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa
     3541 acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa gaatacatgg
     3601 actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca ggcgttctga
     3661 tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag gctgtggaca
     3721 tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc agcgagatcg
     3781 cgcgctggct gtcgacgctt gagatttccg gctccggact cagatcacga gctcaagctt
     3841 caaattctgc agtcgacggt accgcgggcc cgggatcatc aacaagtttg tacaaaaaag
     3901 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     3961 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
     4021 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     4081 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     4141 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     4201 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     4261 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     4321 tcatccggag ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     4381 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     4441 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     4501 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     4561 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     4621 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     4681 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     4741 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     4801 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     4861 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     4921 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     4981 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     5041 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     5101 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     5161 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     5221 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     5281 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     5341 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     5401 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     5461 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     5521 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     5581 agtggttgat gatcctaggt gagtctagag agttaattaa gacccgggac tagtccctag
     5641 agtcctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc
     5701 tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg
     5761 gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt
     5821 tcaatttact gattgtaccc tactacttat atgtacaata ttaaaatgaa aacaatatat
     5881 tgtgctgaat aggtttatag cgacatctat gatagagcgc cacaataaca aacaattgcg
     5941 ttttattatt acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact
     6001 gattacataa atcttattca aatttcaaaa gtgccccagg ggctagtatc tacgacacac
     6061 cgagcggcga actaataacg ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg
     6121 gtgagattcc ttgaagttga gtattggccg tccgctctac cgaaagttac gggcaccatt
     6181 caacccggtc cagcacggcg gccgggtaac cgacttgctg ccccgagaat tatgcagcat
     6241 ttttttggtg tatgtgggcc ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa
     6301 tcgttgggcg ggtccagggc gaattttgcg acaacatgtc gaggctcagc aggacctgca
     6361 ggcatgcacg cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6421 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6481 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
     6541 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     6601 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     6661 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     6721 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     6781 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     6841 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     6901 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     6961 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     7021 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     7081 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     7141 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     7201 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     7261 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     7321 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     7381 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     7441 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     7501 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     7561 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     7621 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     7681 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
     7741 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     7801 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     7861 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     7921 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     7981 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     8041 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     8101 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     8161 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     8221 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     8281 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     8341 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     8401 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     8461 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     8521 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     8581 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     8641 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     8701 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     8761 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     8821 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     8881 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
     8941 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     9001 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     9061 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     9121 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     9181 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     9241 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     9301 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     9361 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     9421 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     9481 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     9541 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     9601 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     9661 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     9721 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     9781 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     9841 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     9901 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     9961 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
    10021 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
    10081 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
    10141 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
    10201 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
    10261 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
    10321 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
    10381 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
    10441 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
    10501 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    10561 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    10621 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
    10681 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10741 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
    10801 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    10861 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    10921 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    10981 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    11041 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    11101 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    11161 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    11221 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    11281 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    11341 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    11401 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    11461 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    11521 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    11581 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    11641 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
    11701 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
    11761 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
    11821 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    11881 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    11941 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    12001 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    12061 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    12121 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    12181 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    12241 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    12301 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    12361 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    12421 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    12481 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    12541 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    12601 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    12661 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    12721 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    12781 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    12841 ccgagctgcc ggtcggggag ctgttggctg gctgg
//
