LOCUS       sequence-42049-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EPB41
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccctctgaat attttgttta actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggccactg caaggtttct ttgttggatg
      121 acacagttta tgaatgtgtt gtggagaaac atgctaaggg acaagatttg cttaaacgag
      181 tatgtgagca tctcaatctt ttggaagaag actattttgg tctagccatt tgggataacg
      241 caacctctaa gacatggctg gattccgcca aagaaataaa aaagcaggtt cgtggtgtcc
      301 cttggaattt tacatttaat gtaaagtttt atccacctga cccagcacag ttaacagaag
      361 acataacaag atattattta tgtcttcagc ttcggcagga catagttgca ggacgtctgc
      421 cctgttcctt tgcaacctta gcattattag gttcttacac catccagtct gaactgggag
      481 actacgaccc agaactccat ggcgtggatt atgttagtga ttttaaactg gccccgaatc
      541 agaccaagga acttgaagag aaggtcatgg aactgcataa gtcatacagg tccatgactc
      601 cagctcaggc tgacttggag tttcttgaga atgccaaaaa gttgtctatg tatggagttg
      661 atcttcataa agcaaaggac ttggaaggag tagatatcat cctaggtgtc tgctctagtg
      721 gccttctggt ttacaaagat aagctgagaa ttaaccgctt cccttggccc aaagtgctg
//
