LOCUS       sequence-420491-       14029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter expression of N-terminal 
            mNeonGreen fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14029)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 14029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pUBQ10-mNeonGreen-GW
FEATURES             Location/Qualifiers
     source          1..14029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    109..133
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(219..471)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(481..1032)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1038..1418)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    3806..3930
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3955..3960,3961..3978,3979..3985)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4039..4698
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4105..4124)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5040..5345
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5224..5243
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5386..5510)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5569..6276
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6301..6318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6301..6317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6310..6332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6520..6544
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7845..8474
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8903..9976
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10042..10236
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10472..10494)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10580..10720
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10635..10654
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10735..10752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10906..11494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10986..11005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12919..13713)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
       61 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
      121 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
      181 gtactgaatt aacgccgaat taattcgagc tcggatctga taatttattt gaaaattcat
      241 aagaaaagca aacgttacat gaattgatga aacaatacaa agacagataa agccacgcac
      301 atttaggata ttggccgaga ttactgaata ttgagtaaga tcacggaatt tctgacagga
      361 gcatgtcttc aattcagccc aaatggcagt tgaaatactc aaaccgcccc atatgcagga
      421 gcggatcatt cattgtttgt ttggttgcct ttgccaacat gggagtccaa gattctgcag
      481 tcaaatctcg gtgacgggca ggaccggacg gggcggtacc ggcaggctga agtccagctg
      541 ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg gccgcccgca gcatgccgcg
      601 gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac
      661 agcgaccacg ctcttgaagc cctgtgcctc cagggacttc agcaggtggg tgtagagcgt
      721 ggagcccagt cccgtccgct ggtggcgggg ggagacgtac acggtcgact cggccgtcca
      781 gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg atgccggcga cctcgccgtc
      841 cacctcggcg acgagccagg gatagcgctc ccgcagacgg acgaggtcgt ccgtccactc
      901 ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt agtggttgac
      961 gatggtgcag accgccggca tgtccgcctc ggtggcacgg cggatgtcgg ccgggcgtcg
     1021 ttctgggctc atcgattcga tttggtgtat cgagattggt tatgaaattc agatgctagt
     1081 gtaatgtatt ggtaatttgg gaagatataa taggaagcaa ggctatttat ccatttctga
     1141 aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc gttcttgctg taaagcgttg
     1201 tttggtacac ttttgactag cgaggcttgg cgtgtcagcg tatctattca aaagtcgtta
     1261 atggctgcgg atcaagaaaa agttggaata gaaacagaat acccgcgaaa ttcaggcccg
     1321 gttgccatgt cctacacgcc gaaataaacg accaaattag tagaaaaata aaaactgact
     1381 cggatactta cgtcacgtct tgcgcactga tttgaaaaat ctcagaattc gcgacgtcgt
     1441 atgatcgcga agcacccacc ctaagccaca ttttaatcct tctgttggcc atgccccatc
     1501 aaagttgcac ttaacccaag attgtggtgg agcttcccat gtttctcgtc tgtcccgacg
     1561 gtgttgtggt tggtgctttc cttacattct gagcctcttt ccttctaatc cactcatctg
     1621 catcttcttg tgtccttact aatacctcat tggttccaaa ttccctccct ttaagcacca
     1681 gctcgtttct gttcttccac agcctcccaa gtatccaagg gactaaagcc tccacattct
     1741 tcagatcagg atattcttgt ttaagatgtt gaactctatg gaggtttgta tgaactgatg
     1801 atctaggacc ggataagttc ccttcttcat agcgaactta ttcaaagaat gttttgtgta
     1861 tcattcttgt tacattgtta ttaatgaaaa aatattattg gtcattggac tgaacacgag
     1921 tgttaaatat ggaccaggcc ccaaataaga tccattgata tatgaattaa ataacaagaa
     1981 taaatcgagt caccaaacca cttgcctttt ttaacgagac ttgttcacca acttgataca
     2041 aaagtcatta tcctatgcaa atcaataatc atacaaaaat atccaataac actaaaaaat
     2101 taaaagaaat ggataatttc acaatatgtt atacgataaa gaagttactt ttccaagaaa
     2161 ttcactgatt ttataagccc acttgcatta gataaatggc aaaaaaaaac aaaaaggaaa
     2221 agaaataaag cacgaagaat tctagaaaat acgaaatacg cttcaatgca gtgggaccca
     2281 cggttcaatt attgccaatt ttcagctcca ccgtatattt aaaaaataaa acgataatgc
     2341 taaaaaaata taaatcgtaa cgatcgttaa atctcaacgg ctggatctta tgacgaccgt
     2401 tagaaattgt ggttgtcgac gagtcagtaa taaacggcgt caaagtggtt gcagccggca
     2461 cacacgagtc gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg
     2521 caattagcca aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat
     2581 tagctattgc ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt
     2641 cttcttctat aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc
     2701 tcaaaatctt aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt
     2761 tccttattct ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc
     2821 tttggtttag attctgttaa tcttagatcg aagacgattt tctgggtttg atcgttagat
     2881 atcatcttaa ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga
     2941 gttttgtcga ataattactc ttcgatttgt gatttctatc tagatctggt gttagtttct
     3001 agtttgtgcg atcgaatttg tcctcgagat ggttagtaag ggggaagagg ataacatggc
     3061 atcactcccg gctacccatg aactgcacat atttggaagt attaacggcg ttgattttga
     3121 catggtcggc cagggcacgg gaaaccccaa cgacggatat gaggagctta atcttaaaag
     3181 tacgaaggga gatttgcagt tctctccctg gatactggtc ccgcatatcg ggtacggttt
     3241 ccatcaatat ctgccctacc ccgacggtat gagtcctttc caggctgcaa tggtggacgg
     3301 ctctggctac caagttcata ggacgatgca attcgaggac ggtgcaagtc ttacagtcaa
     3361 ttatagatac acatatgagg gttctcacat aaagggagag gcccaggtga aagggacggg
     3421 cttccccgct gatggccccg tgatgacaaa ctcactcacg gcagccgact ggtgtaggtc
     3481 taaaaagact taccctaacg acaagacgat tatatcaaca ttcaaatgga gctacaccac
     3541 tgggaatggc aagcgttaca gaagtactgc caggacaaca tacacttttg cgaagcccat
     3601 ggctgctaac tatctaaaaa accaaccaat gtatgttttc agaaaaaccg agcttaagca
     3661 cagtaagact gaactaaact ttaaagagtg gcaaaaggct tttacggatg tcatgggtat
     3721 ggacgagctt tacaagtccg gactcagatc acgagctcaa gcttcaaatt ctgcagtcga
     3781 cggtaccgcg ggcccgggat catcaacaag tttgtacaaa aaagctgaac gagaaacgta
     3841 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     3901 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     3961 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     4021 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     4081 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     4141 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     4201 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggagttccgt
     4261 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     4321 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     4381 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     4441 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     4501 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     4561 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     4621 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     4681 tggcagggcg gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg
     4741 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     4801 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     4861 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     4921 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     4981 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     5041 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     5101 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     5161 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     5221 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     5281 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     5341 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     5401 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     5461 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatgatcct
     5521 aggtgagtct agagagttaa ttaagacccg ggactagtcc ctagagtcct gctttaatga
     5581 gatatgcgag acgcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta
     5641 aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat
     5701 atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt
     5761 accctactac ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt
     5821 atagcgacat ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat
     5881 ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta
     5941 ttcaaatttc aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat
     6001 aacgctcact gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag
     6061 ttgagtattg gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac
     6121 ggcggccggg taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg
     6181 ggccccaaat gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca
     6241 gggcgaattt tgcgacaaca tgtcgaggct cagcaggacc tgcaggcatg cacgcttggc
     6301 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     6361 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     6421 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
     6481 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
     6541 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     6601 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     6661 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     6721 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     6781 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     6841 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     6901 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     6961 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     7021 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     7081 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     7141 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     7201 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     7261 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     7321 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     7381 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     7441 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     7501 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     7561 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     7621 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     7681 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     7741 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     7801 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     7861 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     7921 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     7981 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     8041 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     8101 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     8161 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     8221 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     8281 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     8341 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     8401 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     8461 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     8521 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     8581 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     8641 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     8701 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     8761 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     8821 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     8881 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     8941 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     9001 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     9061 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     9121 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     9181 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     9241 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     9301 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     9361 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     9421 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     9481 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     9541 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     9601 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     9661 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     9721 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     9781 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     9841 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     9901 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     9961 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
    10021 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
    10081 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
    10141 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
    10201 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
    10261 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
    10321 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
    10381 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
    10441 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10501 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10561 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
    10621 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10681 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
    10741 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10801 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    11041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11101 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11341 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11521 gtctgacgct cagtgctgag agatcccctc ataatttccc caaagcgtaa ccatgtgtga
    11581 ataaattttg agctagtagg gttgcagcca cgagtaagtc ttcccttgtt attgtgtagc
    11641 cagaatgccg caaaacttcc atgcctaagc gaactgttga gagtacgttt cgatttctga
    11701 ctgtgttagc ctggaagtgc ttgtcccaac cttgtttctg agcatgaacg cccgcaagcc
    11761 aacatgttag ttgaagcatc agggcgatta gcagcatgat atcaaaacgc tctgagctgc
    11821 tcgttcggct atggcgtagg cctagtccgt aggcaggact tttcaagtct cggaaggttt
    11881 cttcaatctg cattcgcttc gaatagatat taacaagttg tttgggtgtt cgaatttcaa
    11941 caggtaagtt agttgctaga atccatggct cctttgccga cgctgagtag attttaggtg
    12001 acgggtggtg acaatgagtc cgtgtcgagc gctgattttt tcggccttta gagcgagatt
    12061 tatacaatag aatttggcat gagattggat tgcttttagt cagcctctta tagcctaaag
    12121 tctttgagtg actagatgac atatcatgta agttgctgat aggtttccag ttttccgctc
    12181 ctaggtctgc atattgtact tttcctctta ctcgacttaa ccagtaccaa cccagcttct
    12241 caacggattt ataccatggc actttaaagc cagcatcact gacaatgagc ggtgtggtgt
    12301 tactcggtag aatgctcgca aggtcggcta gaaattggtc atgagctttc tttgaacatt
    12361 gctctgaaag cgggaacgct ttctcataaa gagtaacaga acgaccgtgt agtgcgactg
    12421 aagctcgcaa taccataagc cgtttttgct cacggatatc agaccagtca acaagtacaa
    12481 tgggcatcgt attgcccgaa cagataaagc tagcatgcca acggtataca gcgagtcgct
    12541 ctttgtggag gtgacgatta cctaacaatc ggtcgattcg tttgatgtta tgttttgttc
    12601 tcgctttggt tggcaggtta cggccaagtt cggtaagagt gagagtttta cagtcaagta
    12661 aggcgtggca agccaacgtt aagctgttga gtcgttttaa gtgtaattcg gggcagaatt
    12721 ggtaaagaga gtcgtgtaaa atatcgagtt cgcacatttt gttgtctgat tattgatttt
    12781 tggcgaaacc atttgatcat atgacaagat gtgtatctac cttaacttaa tgattttgat
    12841 aaaaatcatt aggggattca tcagcgctca gtggaacgaa aactcacgtt aagggatttt
    12901 ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    12961 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    13021 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    13081 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    13141 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    13201 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    13261 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    13321 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    13381 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    13441 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    13501 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    13561 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    13621 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    13681 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    13741 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    13801 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    13861 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
    13921 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
    13981 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctg
//
