LOCUS       sequence-42050-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UHRF1
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             495..512
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(565..583)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      579..626
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttccctctga atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggttccg cgtggtagtg agccctacag tctcacggcc
      121 cagcagagca gcctcatcag agaggacaag agcaacgcca agctgtggaa tgaggtcctg
      181 gcgtcactca aggaccggcc ggcgagcggc agcccgttcc agttgttcct gagtaaagtg
      241 gaggagacgt tccagtgtat ctgctgtcag gagctggtgt tccggcccat cacgaccgtg
      301 tgccagcaca acgtgtgcaa ggactgcctg gacagatcct ttcgggcaca ggtgttcagc
      361 tgccctgcct gccgctacga cctgggccgc agctatgcca tgcaggtgaa ccagcctctg
      421 cagaccgtcc tcaaccagct cttccccggc tacggcaatg gccggtgatg acgaagcttg
      481 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      541 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      601 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      661 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttac
//
